2024年3月13日发(作者：苏英喆)
Vector
PMD18-T
PMD19-T
PMD20-T
pEASY-T
pUcm-T
pCR2.1-TOPO
pGEM-T(-Easy)
pBS-T
pUC18(19)/118(119)/57
Primer(F)
M13F(-47)
M13F(-47)
M13F(-47)
M13F/T7
M13F/T7
M13F/T7
M13F/T7
M13F/T7
M13F(-47)
T7
T7
T7
Primer(R)
M13R(-48)
M13R(-48)
M13R(-48)
M13R
M13R
M13R
SP6/M13R
T3/M13R
M13R(-48)
T7 Ter
T7 Ter
T7 Ter
T7 Ter
T7ter
T7ter
T7ter
T7ter
T7ter
T7ter
T7ter
T7ter
pGEX-3'
pGEX-3'
pGEX-3'
pBV220R
SP6/BGH
pET-*(His)(KAN+)
pET22(a,b,c)(AMP+)
pET28,30,24,29(KAN+)
pET32(a,b,c) (AMP+)
pET39(kan+)
pET40(kan+)
pET41(kan+)
pET42(kan+)
pET48(kan+)
pET49(kan+)
pET50(kan+)
pEASY-E1(AMP+)
pGEX-*TK
pGEX-*X
pGEX-*P
pTrcHis
pcDNA3.0
/
/
/
T7
pGEX-5'
pGEX-5'
pGEX-5'
M13R(-48)
CMV-F/pEGFP-N5'/T7
Vector
pcDNA3.1
pcDNA4
pcDNA6
pcDNAII
pBluescript SK/KS（+/-）
pGCSilencer-U6/Neo/DsRed
Primer(F)
CMV-F/pEGFP-N5'/T7
CMV-F/pEGFP-N5'/T7
CMV-F/pEGFP-N5'/T7
T7
M13F/T7
U6-5'
S1
M13F/T7
M13F
T7
M13F
T7
Pinpoint Primer
RVP3
M13R(-48)
M13F(-47
T7
M13F/T7
T3
pEGFP-N-5'/CMV-30
pEGFP-N-5
’
pEGFP-C-5
’
pEGFP-N-5'
/
M13R/M13R(-48)
pEGFP-C-5’
pEGFP-N-5
’
Primer(R)
BGH
BGH
BGH
SP6
T3/M13R
PCANTB5E
PCR2.1-TOPO
pDonar
pDrive
pENTR/D-TOPO(KAN+)
pGBKT7(Kana+)
PinpointTM
pGL3
pPROEXHTA
pSilencer3.1-H1
pSUPER
pCMV-Sport
pCMV-Tag(KAN+)
pFlag-cmv
pEG/Y/CFP-N(KAN+)
pEG/Y/CFP-C(KAN+)
pIRES2-EGFP(KAN+)
pEG/Y/CFP-1(KAN+)
pEG/Y/CFP(5'MCS)
pEG/Y/CFP(3'MCS)
pAcGFP1-N(KAN+)
S6
M13R
M13R
SP6
M13R
3'BD
SP6
GLP2
/
3.0REV
T3
SP6/M13R
T7
CMV-24
pEGFP-N-3
’
pEGFP-C-3
’
pIRES2-EGFP-R
pEGFP-N-3
’
pEGFP-N-3’
pGEX-3'
/
Vector
pAcGFP1-C(KAN+)
pPIC9K
pPIC3.5K
pPICZ A/B/C
pPICZaA/B/C
pGADT7-Rec
pGADT7 AD
pGAPZa-A/B/C
pGBKT7(KAN+)
pACT2 AD
pACT
Primer(F)
/
Primer(R)
pEGFP-C-3
’
3’AOX
3'AOX
3'AOX
3'AOX
3'AD
3'AD
3'AOX
3'BD/M13R
pACT2-F
T3
/
M13F/M13F(-47)
/
无反向引物,自己设计引物为
5’AOX/a-factor
5'AOX
5'AOX
5’AOX/a-factor
pACT2-F/T7
T7
a-factor
T7
3'AD(不推荐）
T7
pMSCV-neopLXSN-5'
pCAMBIA1301/1302/1303/1
M13R(-48)/M13R
390/1391(KAN+)
pBI101/121(KAN+)M13R(-48)/M13R
pDNR-LIB(CM+)
psilencer4.1-cmv neo
pGL3-Basic
pVAX1
pTA2
pGL3-Enchancer
pDSRED-N1(KAN+)
pDsRED1-C1
pDsRED2-C1(KAN+)
PCR3.1
pDonar
PEGFP-N1
PEGFP-C1
M13F/T7
M13F(-47)/M13F
RVP3
T7
M13F/T7
RVP3
pEGFP-N-5'
pDsRED-ex-C1-F
pDsRED-ex-C1-F
T7
M13F
PEGFP-N5
PEGFP-C1-5
pDNR-LIB-R
pEGFP-N-5'
GLP2
BGH
T3/M13R
GLP2
pDSRED-N1-R
pEGFP-c-3’(距离
很近,一般不用)
pEGFP-c-3’
BGH
M13R
PEGFP-N-3
PEGFP-C1-3
Vector
pMAL-c2E
pGL2
pQE30or40
pRSET
pSILENCEV2.0-U6
Primer(F)
MalE primer
GLP1
PQE30F
T7
T7
Primer(R)
M13F(-47)
GLP2
PQE30R
T7ter
2.0REV
2024年3月13日发(作者：苏英喆)
Vector
PMD18-T
PMD19-T
PMD20-T
pEASY-T
pUcm-T
pCR2.1-TOPO
pGEM-T(-Easy)
pBS-T
pUC18(19)/118(119)/57
Primer(F)
M13F(-47)
M13F(-47)
M13F(-47)
M13F/T7
M13F/T7
M13F/T7
M13F/T7
M13F/T7
M13F(-47)
T7
T7
T7
Primer(R)
M13R(-48)
M13R(-48)
M13R(-48)
M13R
M13R
M13R
SP6/M13R
T3/M13R
M13R(-48)
T7 Ter
T7 Ter
T7 Ter
T7 Ter
T7ter
T7ter
T7ter
T7ter
T7ter
T7ter
T7ter
T7ter
pGEX-3'
pGEX-3'
pGEX-3'
pBV220R
SP6/BGH
pET-*(His)(KAN+)
pET22(a,b,c)(AMP+)
pET28,30,24,29(KAN+)
pET32(a,b,c) (AMP+)
pET39(kan+)
pET40(kan+)
pET41(kan+)
pET42(kan+)
pET48(kan+)
pET49(kan+)
pET50(kan+)
pEASY-E1(AMP+)
pGEX-*TK
pGEX-*X
pGEX-*P
pTrcHis
pcDNA3.0
/
/
/
T7
pGEX-5'
pGEX-5'
pGEX-5'
M13R(-48)
CMV-F/pEGFP-N5'/T7
Vector
pcDNA3.1
pcDNA4
pcDNA6
pcDNAII
pBluescript SK/KS（+/-）
pGCSilencer-U6/Neo/DsRed
Primer(F)
CMV-F/pEGFP-N5'/T7
CMV-F/pEGFP-N5'/T7
CMV-F/pEGFP-N5'/T7
T7
M13F/T7
U6-5'
S1
M13F/T7
M13F
T7
M13F
T7
Pinpoint Primer
RVP3
M13R(-48)
M13F(-47
T7
M13F/T7
T3
pEGFP-N-5'/CMV-30
pEGFP-N-5
’
pEGFP-C-5
’
pEGFP-N-5'
/
M13R/M13R(-48)
pEGFP-C-5’
pEGFP-N-5
’
Primer(R)
BGH
BGH
BGH
SP6
T3/M13R
PCANTB5E
PCR2.1-TOPO
pDonar
pDrive
pENTR/D-TOPO(KAN+)
pGBKT7(Kana+)
PinpointTM
pGL3
pPROEXHTA
pSilencer3.1-H1
pSUPER
pCMV-Sport
pCMV-Tag(KAN+)
pFlag-cmv
pEG/Y/CFP-N(KAN+)
pEG/Y/CFP-C(KAN+)
pIRES2-EGFP(KAN+)
pEG/Y/CFP-1(KAN+)
pEG/Y/CFP(5'MCS)
pEG/Y/CFP(3'MCS)
pAcGFP1-N(KAN+)
S6
M13R
M13R
SP6
M13R
3'BD
SP6
GLP2
/
3.0REV
T3
SP6/M13R
T7
CMV-24
pEGFP-N-3
’
pEGFP-C-3
’
pIRES2-EGFP-R
pEGFP-N-3
’
pEGFP-N-3’
pGEX-3'
/
Vector
pAcGFP1-C(KAN+)
pPIC9K
pPIC3.5K
pPICZ A/B/C
pPICZaA/B/C
pGADT7-Rec
pGADT7 AD
pGAPZa-A/B/C
pGBKT7(KAN+)
pACT2 AD
pACT
Primer(F)
/
Primer(R)
pEGFP-C-3
’
3’AOX
3'AOX
3'AOX
3'AOX
3'AD
3'AD
3'AOX
3'BD/M13R
pACT2-F
T3
/
M13F/M13F(-47)
/
无反向引物,自己设计引物为
5’AOX/a-factor
5'AOX
5'AOX
5’AOX/a-factor
pACT2-F/T7
T7
a-factor
T7
3'AD(不推荐）
T7
pMSCV-neopLXSN-5'
pCAMBIA1301/1302/1303/1
M13R(-48)/M13R
390/1391(KAN+)
pBI101/121(KAN+)M13R(-48)/M13R
pDNR-LIB(CM+)
psilencer4.1-cmv neo
pGL3-Basic
pVAX1
pTA2
pGL3-Enchancer
pDSRED-N1(KAN+)
pDsRED1-C1
pDsRED2-C1(KAN+)
PCR3.1
pDonar
PEGFP-N1
PEGFP-C1
M13F/T7
M13F(-47)/M13F
RVP3
T7
M13F/T7
RVP3
pEGFP-N-5'
pDsRED-ex-C1-F
pDsRED-ex-C1-F
T7
M13F
PEGFP-N5
PEGFP-C1-5
pDNR-LIB-R
pEGFP-N-5'
GLP2
BGH
T3/M13R
GLP2
pDSRED-N1-R
pEGFP-c-3’(距离
很近,一般不用)
pEGFP-c-3’
BGH
M13R
PEGFP-N-3
PEGFP-C1-3
Vector
pMAL-c2E
pGL2
pQE30or40
pRSET
pSILENCEV2.0-U6
Primer(F)
MalE primer
GLP1
PQE30F
T7
T7
Primer(R)
M13F(-47)
GLP2
PQE30R
T7ter
2.0REV