2024年3月21日发(作者：宣书南)
专利内容由知识产权出版社提供
专利名称：METHOD AND APPARATUS FOR BULK
ENRICHMENT OF A POPULATION OR
SUBPOPULATION OF CELLS
发明人：KENYON, Norma, S.,RUSSELL, Thomas,
R.,RICORDI, Camillo,ZWERNER, Robert, K.
申请号：EP96943478.0
申请日：19961105
公开号：EP0877941A1
公开日：19981118
摘要：A cell population or subpopulation enrichment procedure (10) for separating
undesired populations or subpopulations from a biological sample (12) utilizing relatively
heavy, dense particles (14) and gravity sedimentation. The particles have one or more
reactants bound thereto which are specific to and will bind with the selected population
or subpopulation to be eliminated from the sample. The particles preferably are mixed
with the sample by repeatedly causing the particles to settle through a substantial
portion of the sample to bind to the selected population. The particles with the bound
selected population to be eliminated then are allowed to preferentially settle in the
sample and the supernatant including the enriched population or subpopulation is
separated from the particles with the population to be eliminated bound thereto. The
enriched population supernatant can then be analyzed, utilized as is, have other
populations or subpopulations removed or have additional amounts of remaining cells of
the previously eliminated populations removed in additional steps.
申请人：COULTER CORPORATION,THE UNIVERSITY OF MIAMI
地址：11800 S.W. 147 Avenue Miami, FL 33196 US,P.O. Box 016960 (M811) Miami,
Florida 33101 US
国籍：US,US
代理机构：Kremer, Simon Mark, et al
更多信息请下载全文后查看
2024年3月21日发(作者：宣书南)
专利内容由知识产权出版社提供
专利名称：METHOD AND APPARATUS FOR BULK
ENRICHMENT OF A POPULATION OR
SUBPOPULATION OF CELLS
发明人：KENYON, Norma, S.,RUSSELL, Thomas,
R.,RICORDI, Camillo,ZWERNER, Robert, K.
申请号：EP96943478.0
申请日：19961105
公开号：EP0877941A1
公开日：19981118
摘要：A cell population or subpopulation enrichment procedure (10) for separating
undesired populations or subpopulations from a biological sample (12) utilizing relatively
heavy, dense particles (14) and gravity sedimentation. The particles have one or more
reactants bound thereto which are specific to and will bind with the selected population
or subpopulation to be eliminated from the sample. The particles preferably are mixed
with the sample by repeatedly causing the particles to settle through a substantial
portion of the sample to bind to the selected population. The particles with the bound
selected population to be eliminated then are allowed to preferentially settle in the
sample and the supernatant including the enriched population or subpopulation is
separated from the particles with the population to be eliminated bound thereto. The
enriched population supernatant can then be analyzed, utilized as is, have other
populations or subpopulations removed or have additional amounts of remaining cells of
the previously eliminated populations removed in additional steps.
申请人：COULTER CORPORATION,THE UNIVERSITY OF MIAMI
地址：11800 S.W. 147 Avenue Miami, FL 33196 US,P.O. Box 016960 (M811) Miami,
Florida 33101 US
国籍：US,US
代理机构：Kremer, Simon Mark, et al
更多信息请下载全文后查看