2024年5月26日发(作者：奈映秋)
CellTrace
™
Cell Proliferation Kits
Catalog nos. C34554, C34557, C34564
Table 1 Contents and storage
Material
CellTrace
™
CSFE (Cat. no. C34554),
CellTrace
Violet (Cat. no. C34557), or
CellTrace
Far Red (Cat. no. C34564) (Component A)
DMSO (Component B)
™
™
Amount
10 vials
9 vials
500 μL
Storage
• ≤–20°C
• Desiccate
• Protect from light
≤–20°C
Stability
When stored as directed, the
product is stable for at least
1 year.
Number of reactions: Sufficient material is supplied for 180 reactions, based on the protocol below.
Approximate fluorescence excitation/emission maxima: CellTrace
™
CSFE: 492/517 in nm; CellTrace
™
Violet: 405/450 in nm;
CellTrace
™
Far Red: 630/661 in nm.
Introduction
The CellTrace
™
Cell Proliferation Kits provide versatile and well-retained cell tracing
reagents in a convenient and easy-to-use form. Each kit contains a CellTrace
™
reagent
(CellTrace
™
CSFE, CellTrace
™
Violet, or CellTrace
™
Far Red) in single-use vials to permit
small scale experiments without preparing excess quantities of stock solution. The
CellTrace
™
reagents easily diffuse into cells and bind covalently to intracellular amines,
resulting in stable, well-retained fluorescent staining that can be fixed with aldehyde
fixatives. Excess unconjugated reagent passively diffuses to the extracellular medium,
where it can be quenched with complete media and washed away.
Spectral CharacteristicsCellTrace
™
CSFE (Cat. no. C34554)
The approximate excitation and emission peaks of this product after hydrolysis are
492 nm and 517 nm, respectively. Cells labeled with CellTrace
™
CSFE reagent can be
visualized by fluorescence microscopy using standard fluorescein filter sets or analyzed
by flow cytometry in an instrument equipped with a 488-nm excitation source.
CellTrace
™
Violet (Cat. no. C34557)
The approximate excitation and emission peaks of this product after hydrolysis are
405 nm and 450 nm, respectively. Cells labeled with CellTrace
™
Violet reagent can be
visualized by fluorescence microscopy using standard DAPI filter sets or analyzed by
flow cytometry in an instrument equipped with a 405-nm excitation source.
CellTrace
™
Far Red (Cat. no. C34564)
The approximate excitation and emission peaks of this product after hydrolysis are
630 nm and 661 nm, respectively. Cells labeled with CellTrace
™
Far Red reagent can be
visualized by fluorescence microscopy using standard Cy
®
5 filter sets or analyzed by
flow cytometry in an instrument equipped with a 633/635-nm excitation source.
For Research Use Only. Not for use in diagnostic procedures.
MAN0002595 | MP34557
Revision: A.0
Figure 1 Generational tracing using CellTrace
™
Reagents. (A) Cell proliferation was followed for 8 generations using the CellTrace
™
Violet reagent. Human
peripheral blood mononuclear cells were harvested and stained with CellTrace
™
Violet reagent prior to stimulation with mouse anti-human CD3 (Cat. no.
MHCD0300) and Interleukin-2 (Cat. no. PHC0027) for 7 days. The discrete peaks in this histogram represent successive generations of live, CD4 positive cells.
Analysis was completed using an Attune
®
Acoustic Focusing Cytometer with 405-nm excitation and a 450/40 bandpass emission filter. The unstimulated
parent generation is indicated in red. (B) and (C) Cell Proliferation was followed for 7 generations using the CellTrace
™
CFSE and CellTrace
™
Far Red reagents,
respectively. Human T lymphocytes were harvested and stained with CellTrace
™
CFSE or CellTrace
™
Far Red reagent prior to stimulation with anti-human CD3
(Cat. no. MHCD0300) for 5 days. The discrete peaks in these histograms represent successive generations of live, SYTOX
®
Green (Cat. no. S34860) negative
cells. The unstimulated parent generation is indicated in blue (CellTrace
™
CFSE) or purple (CellTrace
™
Far Red). Analysis was completed using an Attune
®
Acoustic Focusing Cytometer with 488 nm excitation and a 530/30 nm bandpass emission filter for CellTrace
™
CFSE. Analysis was completed for CellTrace
™
Far Red using an Attune
®
Acoustic Focusing Cytometer with 638 nm excitation and a 660/20 nm bandpass emission filter.
A
4000
100
80
60
40
20
B
100
80
60
40
20
C
C
e
l
l
C
o
u
n
t
3000
2000
1000
P
e
r
c
e
n
t
o
f
M
a
x
P
e
r
c
e
n
t
o
f
M
a
x
0
10
2
10
3
10
4
10
5
10
6
0
10
3
10
4
10
5
10
6
0
10
3
10
4
10
5
10
6
CellTrace
™
Violet Fluorescence
CellTrace
™
CFSE Fluorescence
CellTrace
™
Far Red Fluorescence
Before Starting
Materials Required but Not
Provided
•
•
•
•
Cells of interest as a single-cell suspension
Phosphate-buffered saline (PBS) or similar protein-free buffer
Culture media containing protein such as FBS or BSA
Flow cytometer
Caution
No data are available addressing the mutagenicity or toxicity of CellTrace
™
reagents
(Component A). Handle the DMSO dye solution with caution because DMSO is known
to facilitate the entry of organic molecules into tissues. Always wear suitable protective
clothing, gloves, and eye/face protection when handling this reagent. Dispose of the
reagents in compliance with all pertaining local regulations.
Storage and Handling
Upon receipt, store the kit components desiccated at ≤–20°C until required for use.
When stored properly DMSO and dry CellTrace
™
reagents are stable for at least one
year. Allow the product to warm to room temperature before opening vials. Stock
solutions of CellTrace
™
reagents should be used only on the day of preparation. Avoid
repeated freezing and thawing of kit contents.
CellTrace
™
Cell Proliferation Kits
|
2
2024年5月26日发(作者：奈映秋)
CellTrace
™
Cell Proliferation Kits
Catalog nos. C34554, C34557, C34564
Table 1 Contents and storage
Material
CellTrace
™
CSFE (Cat. no. C34554),
CellTrace
Violet (Cat. no. C34557), or
CellTrace
Far Red (Cat. no. C34564) (Component A)
DMSO (Component B)
™
™
Amount
10 vials
9 vials
500 μL
Storage
• ≤–20°C
• Desiccate
• Protect from light
≤–20°C
Stability
When stored as directed, the
product is stable for at least
1 year.
Number of reactions: Sufficient material is supplied for 180 reactions, based on the protocol below.
Approximate fluorescence excitation/emission maxima: CellTrace
™
CSFE: 492/517 in nm; CellTrace
™
Violet: 405/450 in nm;
CellTrace
™
Far Red: 630/661 in nm.
Introduction
The CellTrace
™
Cell Proliferation Kits provide versatile and well-retained cell tracing
reagents in a convenient and easy-to-use form. Each kit contains a CellTrace
™
reagent
(CellTrace
™
CSFE, CellTrace
™
Violet, or CellTrace
™
Far Red) in single-use vials to permit
small scale experiments without preparing excess quantities of stock solution. The
CellTrace
™
reagents easily diffuse into cells and bind covalently to intracellular amines,
resulting in stable, well-retained fluorescent staining that can be fixed with aldehyde
fixatives. Excess unconjugated reagent passively diffuses to the extracellular medium,
where it can be quenched with complete media and washed away.
Spectral CharacteristicsCellTrace
™
CSFE (Cat. no. C34554)
The approximate excitation and emission peaks of this product after hydrolysis are
492 nm and 517 nm, respectively. Cells labeled with CellTrace
™
CSFE reagent can be
visualized by fluorescence microscopy using standard fluorescein filter sets or analyzed
by flow cytometry in an instrument equipped with a 488-nm excitation source.
CellTrace
™
Violet (Cat. no. C34557)
The approximate excitation and emission peaks of this product after hydrolysis are
405 nm and 450 nm, respectively. Cells labeled with CellTrace
™
Violet reagent can be
visualized by fluorescence microscopy using standard DAPI filter sets or analyzed by
flow cytometry in an instrument equipped with a 405-nm excitation source.
CellTrace
™
Far Red (Cat. no. C34564)
The approximate excitation and emission peaks of this product after hydrolysis are
630 nm and 661 nm, respectively. Cells labeled with CellTrace
™
Far Red reagent can be
visualized by fluorescence microscopy using standard Cy
®
5 filter sets or analyzed by
flow cytometry in an instrument equipped with a 633/635-nm excitation source.
For Research Use Only. Not for use in diagnostic procedures.
MAN0002595 | MP34557
Revision: A.0
Figure 1 Generational tracing using CellTrace
™
Reagents. (A) Cell proliferation was followed for 8 generations using the CellTrace
™
Violet reagent. Human
peripheral blood mononuclear cells were harvested and stained with CellTrace
™
Violet reagent prior to stimulation with mouse anti-human CD3 (Cat. no.
MHCD0300) and Interleukin-2 (Cat. no. PHC0027) for 7 days. The discrete peaks in this histogram represent successive generations of live, CD4 positive cells.
Analysis was completed using an Attune
®
Acoustic Focusing Cytometer with 405-nm excitation and a 450/40 bandpass emission filter. The unstimulated
parent generation is indicated in red. (B) and (C) Cell Proliferation was followed for 7 generations using the CellTrace
™
CFSE and CellTrace
™
Far Red reagents,
respectively. Human T lymphocytes were harvested and stained with CellTrace
™
CFSE or CellTrace
™
Far Red reagent prior to stimulation with anti-human CD3
(Cat. no. MHCD0300) for 5 days. The discrete peaks in these histograms represent successive generations of live, SYTOX
®
Green (Cat. no. S34860) negative
cells. The unstimulated parent generation is indicated in blue (CellTrace
™
CFSE) or purple (CellTrace
™
Far Red). Analysis was completed using an Attune
®
Acoustic Focusing Cytometer with 488 nm excitation and a 530/30 nm bandpass emission filter for CellTrace
™
CFSE. Analysis was completed for CellTrace
™
Far Red using an Attune
®
Acoustic Focusing Cytometer with 638 nm excitation and a 660/20 nm bandpass emission filter.
A
4000
100
80
60
40
20
B
100
80
60
40
20
C
C
e
l
l
C
o
u
n
t
3000
2000
1000
P
e
r
c
e
n
t
o
f
M
a
x
P
e
r
c
e
n
t
o
f
M
a
x
0
10
2
10
3
10
4
10
5
10
6
0
10
3
10
4
10
5
10
6
0
10
3
10
4
10
5
10
6
CellTrace
™
Violet Fluorescence
CellTrace
™
CFSE Fluorescence
CellTrace
™
Far Red Fluorescence
Before Starting
Materials Required but Not
Provided
•
•
•
•
Cells of interest as a single-cell suspension
Phosphate-buffered saline (PBS) or similar protein-free buffer
Culture media containing protein such as FBS or BSA
Flow cytometer
Caution
No data are available addressing the mutagenicity or toxicity of CellTrace
™
reagents
(Component A). Handle the DMSO dye solution with caution because DMSO is known
to facilitate the entry of organic molecules into tissues. Always wear suitable protective
clothing, gloves, and eye/face protection when handling this reagent. Dispose of the
reagents in compliance with all pertaining local regulations.
Storage and Handling
Upon receipt, store the kit components desiccated at ≤–20°C until required for use.
When stored properly DMSO and dry CellTrace
™
reagents are stable for at least one
year. Allow the product to warm to room temperature before opening vials. Stock
solutions of CellTrace
™
reagents should be used only on the day of preparation. Avoid
repeated freezing and thawing of kit contents.
CellTrace
™
Cell Proliferation Kits
|
2