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ZEISS ZEN core v2.6 用户指南说明书

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2024年10月13日发(作者:邬白玉)

Release Notes

ZEISS ZEN core v2.6

ZEISS ZEN core v2.6

Original Manual

Carl Zeiss Microscopy GmbH

Carl-Zeiss-Promenade 10

07745 Jena

Germany

********************

/microscopy

Carl Zeiss Microscopy GmbH

ZEISS Group

Kistlerhofstr. 75

81379 München

Document Name: ZEISS ZEN core v2.6 Release Notes

Revision: 1

Language: en-US

Effective from: 01/2019

© 2019 This document or any part of it must not be translated, reproduced, or transmitted in any form or by any means, elec-

tronic or mechanical, including photocopying, recording, or by any information or retrieval system. Violations will be prose-

cuted. The use of general descriptive names, registered names, trademarks, etc. in this document does not imply, even in the

absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore

free for general use. Software programs will fully remain the property of ZEISS. No program, documentation, or subsequent up-

grade thereof may be disclosed to any third party, unless prior written consent of ZEISS has been procured to do so, nor may be

copied or otherwise duplicated, even for the customer's internal needs, apart from a single back-up copy for safety purposes.

ZEISS reserves the right to make modifications to this document without notice.

1 Release Notes​ZEISS

1Release Notes

New Modules

The following new software modules are available:

Module

Non-metallic

Inclusion Analysis

Description

The new module Non-metallic Inclusion Analysis provides evaluation and

rating of non metallic inclusions in steel (Oxides, Sulfides and Nitrides).

Supported standards:

§ASTM E45

§ISO 4967

§JIS G0555

§GB/T 10561

§EN 10247

§SEP 1571

§DIN 50602

General features:

§Adjustable image acquisition profiles with microscope and camera

settings for the supervisor

§Selectable image acquisition profiles for the operator

§Standard measuring field based definition of the tiles acquisition area

§Automated parallel analysis for all selected standards

§Customer database in Excel table format for input forms

§Efficient auto segmentation for oxide, sulfide and nitride detection

§Artifact filter for automated filtering of scratches

§Automated detection of the main deformation axis

(horizontal, vertical)

§Standard specific characteristic value filter: automated exclusion of

inclusions per defined size class

§Global result view with:

–inclusion gallery for inspection and revision of objects

(oxides, sulfides, nitrides and artifacts)

–several filter/sorting options and overarching result statistics

–standard results selectable by one mouse click

§Field based inspection view with:

–acquired specimen image

–a grid of standard measuring fields as overlay

–specimen and inclusion live navigation for inspection and

revision of objects (oxides, sulfides, nitrides and artifacts)

–several filter- and sorting options per standard

specific inclusion types

–true worst field detection

–result statistics with field rating

–Standard results selectable by one mouse click

§Reports in Microsoft Word (from Microsoft Word 2013) and

export as PDF

§Automated generation of reports/summary report for all standards

§Excel export of result tables

Release Notes ZEISS ZEN core v2.6 | en-US | Rev.13

1 Release Notes​ZEISS

Module

Connect Entry

Description

The new module Connect Entry (module for ZEN starter, integrated in

ZEN core base package) is used for acquisition and correlation of light

and electron microscope images. It offers interactive acquisition and

contextual display of images in single- and multi-instrument workflows.

General features:

§Multi-resolution and multi-modal display of images

§Project management for acquired images

§Positioning of images according to stage coordinates

§Manual alignment of images to allow correction of shift, rotation

and skew

Connect AdvancedThe new module Connect Advanced (module for ZEN starter and ZEN

core) allows to extend the functionality of the integrated module

Connect Entry. The functionality of the module Third-party Import

is included in Connect Advanced.

General features:

§Interactive control of stage movement from

the correlative workspace

§Import of images into projects

§Export of merged project view as image

§Automated alignment of images using algorithms for

key point detection

§Import of 3rd-party microscopy images (powered by Bioformats)

Third-party ImportThe new module Third-party Import (module for ZEN starter and ZEN

core) allows the import of 3rd-party microscope images to ZEN.

General features:

§Import of 3rd-party images in native format including extraction of

relevant metadata

§Support of metadata extraction depends on original format

(powered by Bioformats)

IntellesisThe new module Intellesis provides a trainable segmentation based on

machine learning algorithms using pixel classification.

General features:

§Generation of training models by image labeling

§Image segmentation based on training models

§Generation of image analysis settings based on training models

Intellesis &

Image Analysis

The new module bundle Intellesis & Image Analysis consists of the two

individual modules Intellesis and Image Analysis.

The assistent for creation of an automatic mesurement program is en-

hanced with the machine learning segmentation functionality of the ZEN

module Intellesis.

Data Storage ClientThe new module Data Storage Client (module for ZEN starter and ZEN

core) provides seamless connection as client to ZEN Data Storage Server

for storage of documents and templates.

General features:

§Connection to ZEN Data Storage Server

§Storage and handling of documents and templates

by ZEN Data Storage Server

Release Notes ZEISS ZEN core v2.6 | en-US | Rev.14

1 Release Notes​ZEISS

ModuleDescription

§Centralized user management via ZEN Data Storage Server

IMS ClientThe new module IMS Client (module for ZEN starter and ZEN core;

substitutes the module Data Mangement in ZEN starter and ZEN core)

provides seamless connection to Imageic IMS Database server.

General features:

§Storage and handling of documents and templates

by the IMS Databaser

§Storage of archived data on IMS server system

§Hierarchical structure

§Search / Keyword search

§Client-Server-System

§User management via IMS Server

New Features & Improvements

Area / Application

General

Features / Improvements

Image context menu:

§New short cuts are available to adjust display settings

(Min/Max/Best fit) directly.

Exported images:

§*.jpeg, *.bmp, *.tif, *.png, *.jpegXR have now 300 dpi tag as

standard (previously 96 dpi).

Acquisition:

New task SEM 2D Acquisition available in the group Acquisition for

ZEN core SEM.

Input documents:

New tool Import Bioformats Image available in the Load File task.

APEER ConnectorIncluded built-in connector to APEER - the ZEISS cloud based digital

microscopy platform. Allows easy upload and retrieval of ZEN to the

APEER workflow. The selected APEER workflow will use the selected

ZEN image data as Input.

Microscopes: Micro Toolbox MTB2011 version 2.13.0.3.

WordAddIn is now provided for each user after installation by the ad-

ministrator. It is no longer needed to install it for every single user sepa-

rately. In addition it is now available for non-administrators too.

New features:

§Extended customer logo adaptation (report template)

§More flexibility of column numbers for images (report template)

§Improved form designer: automated customer data import

Devices

Forms and Reports

Browse Job Results

Image Processing

Direkt export of data tables of the selected job to Excel

New processing functions for the Module Intellesis:

§Segmentation

–Intellesis: TrainableSegmentation

(with Minimum Confidence)

§Utilities

Release Notes ZEISS ZEN core v2.6 | en-US | Rev.15

1 Release Notes​ZEISS

Area / ApplicationFeatures / Improvements

–Intellesis: Channels to Labels

–Intellesis: Labels to channels

–Convert Pixel Format, e.g. if a 12-bit image is converted to

8-bit the range 0 to 4095 is mapped to range 0 to 256 (as

opposed to ‘Change Pixel Type’, here the range 4095 to

65535 is filled with zeros and then the range 0 to 65535 is

mapped to 0 to 256)

OAD (Open Appliccation

Development)

OAD examples (Job templates with OAD macros) and an installation in-

struction are available on the DVD 77 in the following folder: Other/

OAD examples The OAD examples can be run without the license for

the module Macro Environment.

New features:

§‘Tiles (measurement area)’ workbench available in the category

‘Acquisition’. Creates tile images on a motorized stage by adding

multiple tile regions with a certain area measurement, e.g. multi sam-

ple acquisition.

§‘Tiles (measurement area)’ and ‘Position list’ workbenches offer

import of position list and tile regions as .CSV file

§Improved focus strategy:

Combination of focus surface with Autofocus.

Module Tiles & Positions

Module Image AnalysisNew features in Image Analysis Wizard:

§Image Segmentation Setup

–Suppress invalid allows to suppress invalid pixels at the

image border

–Min. Hole Area can be defined to fill holes according to the

minimum size

§Features Setup

–Copy To: allows to add/copy the features of one class to

another one (minimum 2 classes) (especially suited for more

complex class structures)

Module Macro Environ-

ment

New functions:

§Segmentation

–Intellesis: TrainableSegmentation

–Intellesis: TrainableSegmentationWithProbabilityMap

–Intellesis: MinimumConfidence

§Utilities

–CreateSubsetAndSplit

–CreateSubsetAndSplitTo Array

§Application

–GetOmeTiffFile: Input data from many life sciences

file formats (Bio-Formats)

Module Shuttle & Find

Module Linkam

The EM acquisition workbenches (tasks) have been moved from the

category ‘Shuttle & Find’ to the category ‘Acquisition’.

The Linkam controller firmware has been updated to

the following versions:

Release Notes ZEISS ZEN core v2.6 | en-US | Rev.16

1 Release Notes​ZEISS

Area / ApplicationFeatures / Improvements

§T95: firmware version 2.91

§T96: firmware version 1.20

Module Data

Management

The module Data Management has been renamed to

ZEN Module IMS Client.

ZEN Data Storage Server

The Data Storage Server provides a database for storage and multi-client access of documents on a

centralized server. ZEN applications can connect to the server as clients to store and retrieve data.

General features:

§

§

§

§

§

§

§

§

Server database solution based on MS-SQL server

Scalable database architecture

Possible integration into existing server landscape

Multi-client concurrent access to data

Server based user and access management

Full archive integration for ZEN core including storage

of documents and templates

Support of the documents formats: Job results, images, reports, data tables, Job templates,

Report templates, Form templates, Analysis templates, Intellesis models, OAD macros

Optional: Compatible with ZEN Module GxP

Release Notes ZEISS ZEN core v2.6 | en-US | Rev.17

Carl Zeiss Microscopy GmbH

Carl-Zeiss-Promenade 10

07745 Jena

Germany

phone: +49 3641 64 3161

fax: +49 3641 64 3439

*************************

/microscopy

.

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2024年10月13日发(作者:邬白玉)

Release Notes

ZEISS ZEN core v2.6

ZEISS ZEN core v2.6

Original Manual

Carl Zeiss Microscopy GmbH

Carl-Zeiss-Promenade 10

07745 Jena

Germany

********************

/microscopy

Carl Zeiss Microscopy GmbH

ZEISS Group

Kistlerhofstr. 75

81379 München

Document Name: ZEISS ZEN core v2.6 Release Notes

Revision: 1

Language: en-US

Effective from: 01/2019

© 2019 This document or any part of it must not be translated, reproduced, or transmitted in any form or by any means, elec-

tronic or mechanical, including photocopying, recording, or by any information or retrieval system. Violations will be prose-

cuted. The use of general descriptive names, registered names, trademarks, etc. in this document does not imply, even in the

absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore

free for general use. Software programs will fully remain the property of ZEISS. No program, documentation, or subsequent up-

grade thereof may be disclosed to any third party, unless prior written consent of ZEISS has been procured to do so, nor may be

copied or otherwise duplicated, even for the customer's internal needs, apart from a single back-up copy for safety purposes.

ZEISS reserves the right to make modifications to this document without notice.

1 Release Notes​ZEISS

1Release Notes

New Modules

The following new software modules are available:

Module

Non-metallic

Inclusion Analysis

Description

The new module Non-metallic Inclusion Analysis provides evaluation and

rating of non metallic inclusions in steel (Oxides, Sulfides and Nitrides).

Supported standards:

§ASTM E45

§ISO 4967

§JIS G0555

§GB/T 10561

§EN 10247

§SEP 1571

§DIN 50602

General features:

§Adjustable image acquisition profiles with microscope and camera

settings for the supervisor

§Selectable image acquisition profiles for the operator

§Standard measuring field based definition of the tiles acquisition area

§Automated parallel analysis for all selected standards

§Customer database in Excel table format for input forms

§Efficient auto segmentation for oxide, sulfide and nitride detection

§Artifact filter for automated filtering of scratches

§Automated detection of the main deformation axis

(horizontal, vertical)

§Standard specific characteristic value filter: automated exclusion of

inclusions per defined size class

§Global result view with:

–inclusion gallery for inspection and revision of objects

(oxides, sulfides, nitrides and artifacts)

–several filter/sorting options and overarching result statistics

–standard results selectable by one mouse click

§Field based inspection view with:

–acquired specimen image

–a grid of standard measuring fields as overlay

–specimen and inclusion live navigation for inspection and

revision of objects (oxides, sulfides, nitrides and artifacts)

–several filter- and sorting options per standard

specific inclusion types

–true worst field detection

–result statistics with field rating

–Standard results selectable by one mouse click

§Reports in Microsoft Word (from Microsoft Word 2013) and

export as PDF

§Automated generation of reports/summary report for all standards

§Excel export of result tables

Release Notes ZEISS ZEN core v2.6 | en-US | Rev.13

1 Release Notes​ZEISS

Module

Connect Entry

Description

The new module Connect Entry (module for ZEN starter, integrated in

ZEN core base package) is used for acquisition and correlation of light

and electron microscope images. It offers interactive acquisition and

contextual display of images in single- and multi-instrument workflows.

General features:

§Multi-resolution and multi-modal display of images

§Project management for acquired images

§Positioning of images according to stage coordinates

§Manual alignment of images to allow correction of shift, rotation

and skew

Connect AdvancedThe new module Connect Advanced (module for ZEN starter and ZEN

core) allows to extend the functionality of the integrated module

Connect Entry. The functionality of the module Third-party Import

is included in Connect Advanced.

General features:

§Interactive control of stage movement from

the correlative workspace

§Import of images into projects

§Export of merged project view as image

§Automated alignment of images using algorithms for

key point detection

§Import of 3rd-party microscopy images (powered by Bioformats)

Third-party ImportThe new module Third-party Import (module for ZEN starter and ZEN

core) allows the import of 3rd-party microscope images to ZEN.

General features:

§Import of 3rd-party images in native format including extraction of

relevant metadata

§Support of metadata extraction depends on original format

(powered by Bioformats)

IntellesisThe new module Intellesis provides a trainable segmentation based on

machine learning algorithms using pixel classification.

General features:

§Generation of training models by image labeling

§Image segmentation based on training models

§Generation of image analysis settings based on training models

Intellesis &

Image Analysis

The new module bundle Intellesis & Image Analysis consists of the two

individual modules Intellesis and Image Analysis.

The assistent for creation of an automatic mesurement program is en-

hanced with the machine learning segmentation functionality of the ZEN

module Intellesis.

Data Storage ClientThe new module Data Storage Client (module for ZEN starter and ZEN

core) provides seamless connection as client to ZEN Data Storage Server

for storage of documents and templates.

General features:

§Connection to ZEN Data Storage Server

§Storage and handling of documents and templates

by ZEN Data Storage Server

Release Notes ZEISS ZEN core v2.6 | en-US | Rev.14

1 Release Notes​ZEISS

ModuleDescription

§Centralized user management via ZEN Data Storage Server

IMS ClientThe new module IMS Client (module for ZEN starter and ZEN core;

substitutes the module Data Mangement in ZEN starter and ZEN core)

provides seamless connection to Imageic IMS Database server.

General features:

§Storage and handling of documents and templates

by the IMS Databaser

§Storage of archived data on IMS server system

§Hierarchical structure

§Search / Keyword search

§Client-Server-System

§User management via IMS Server

New Features & Improvements

Area / Application

General

Features / Improvements

Image context menu:

§New short cuts are available to adjust display settings

(Min/Max/Best fit) directly.

Exported images:

§*.jpeg, *.bmp, *.tif, *.png, *.jpegXR have now 300 dpi tag as

standard (previously 96 dpi).

Acquisition:

New task SEM 2D Acquisition available in the group Acquisition for

ZEN core SEM.

Input documents:

New tool Import Bioformats Image available in the Load File task.

APEER ConnectorIncluded built-in connector to APEER - the ZEISS cloud based digital

microscopy platform. Allows easy upload and retrieval of ZEN to the

APEER workflow. The selected APEER workflow will use the selected

ZEN image data as Input.

Microscopes: Micro Toolbox MTB2011 version 2.13.0.3.

WordAddIn is now provided for each user after installation by the ad-

ministrator. It is no longer needed to install it for every single user sepa-

rately. In addition it is now available for non-administrators too.

New features:

§Extended customer logo adaptation (report template)

§More flexibility of column numbers for images (report template)

§Improved form designer: automated customer data import

Devices

Forms and Reports

Browse Job Results

Image Processing

Direkt export of data tables of the selected job to Excel

New processing functions for the Module Intellesis:

§Segmentation

–Intellesis: TrainableSegmentation

(with Minimum Confidence)

§Utilities

Release Notes ZEISS ZEN core v2.6 | en-US | Rev.15

1 Release Notes​ZEISS

Area / ApplicationFeatures / Improvements

–Intellesis: Channels to Labels

–Intellesis: Labels to channels

–Convert Pixel Format, e.g. if a 12-bit image is converted to

8-bit the range 0 to 4095 is mapped to range 0 to 256 (as

opposed to ‘Change Pixel Type’, here the range 4095 to

65535 is filled with zeros and then the range 0 to 65535 is

mapped to 0 to 256)

OAD (Open Appliccation

Development)

OAD examples (Job templates with OAD macros) and an installation in-

struction are available on the DVD 77 in the following folder: Other/

OAD examples The OAD examples can be run without the license for

the module Macro Environment.

New features:

§‘Tiles (measurement area)’ workbench available in the category

‘Acquisition’. Creates tile images on a motorized stage by adding

multiple tile regions with a certain area measurement, e.g. multi sam-

ple acquisition.

§‘Tiles (measurement area)’ and ‘Position list’ workbenches offer

import of position list and tile regions as .CSV file

§Improved focus strategy:

Combination of focus surface with Autofocus.

Module Tiles & Positions

Module Image AnalysisNew features in Image Analysis Wizard:

§Image Segmentation Setup

–Suppress invalid allows to suppress invalid pixels at the

image border

–Min. Hole Area can be defined to fill holes according to the

minimum size

§Features Setup

–Copy To: allows to add/copy the features of one class to

another one (minimum 2 classes) (especially suited for more

complex class structures)

Module Macro Environ-

ment

New functions:

§Segmentation

–Intellesis: TrainableSegmentation

–Intellesis: TrainableSegmentationWithProbabilityMap

–Intellesis: MinimumConfidence

§Utilities

–CreateSubsetAndSplit

–CreateSubsetAndSplitTo Array

§Application

–GetOmeTiffFile: Input data from many life sciences

file formats (Bio-Formats)

Module Shuttle & Find

Module Linkam

The EM acquisition workbenches (tasks) have been moved from the

category ‘Shuttle & Find’ to the category ‘Acquisition’.

The Linkam controller firmware has been updated to

the following versions:

Release Notes ZEISS ZEN core v2.6 | en-US | Rev.16

1 Release Notes​ZEISS

Area / ApplicationFeatures / Improvements

§T95: firmware version 2.91

§T96: firmware version 1.20

Module Data

Management

The module Data Management has been renamed to

ZEN Module IMS Client.

ZEN Data Storage Server

The Data Storage Server provides a database for storage and multi-client access of documents on a

centralized server. ZEN applications can connect to the server as clients to store and retrieve data.

General features:

§

§

§

§

§

§

§

§

Server database solution based on MS-SQL server

Scalable database architecture

Possible integration into existing server landscape

Multi-client concurrent access to data

Server based user and access management

Full archive integration for ZEN core including storage

of documents and templates

Support of the documents formats: Job results, images, reports, data tables, Job templates,

Report templates, Form templates, Analysis templates, Intellesis models, OAD macros

Optional: Compatible with ZEN Module GxP

Release Notes ZEISS ZEN core v2.6 | en-US | Rev.17

Carl Zeiss Microscopy GmbH

Carl-Zeiss-Promenade 10

07745 Jena

Germany

phone: +49 3641 64 3161

fax: +49 3641 64 3439

*************************

/microscopy

.

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