2024年3月19日发(作者：员乐松)
专利内容由知识产权出版社提供
专利名称：Production of alpha-ABeta
发明人：Denis Drapeau,Yen-Tung Luan,James R.
Mercer,Wenge Wang,Daniel R. Lasko
申请号：US11213317
申请日：20050825
公开号：US2A1
公开日：20060720
摘要：An improved system for large scale production of proteins and/or polypeptides
in cell culture, particularly in media characterized by one or more of: i) a cumulative amino
acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to
cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to
cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic
ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined
cumulative glutamine and cumulative asparagine concentration between about 16 and 36
mM, is provided. The use of such a system allows high levels of protein production and
lessens accumulation of certain undesirable factors such as ammonium and/or lactate.
Additionally, culture methods including a temperature shift, typically including a decrease
in temperature when the culture has reached about 20-80% of it maximal cell density, are
provided. Alternatively or additionally, the present invention provides methods such that,
after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.
申请人：Denis Drapeau,Yen-Tung Luan,James R. Mercer,Wenge Wang,Daniel R. Lasko
地址：Salem NH US,Chelmsford MA US,Derry NH US,North Chelmsford MA
US,Medford MA US
国籍：US,US,US,US,US
更多信息请下载全文后查看
2024年3月19日发(作者：员乐松)
专利内容由知识产权出版社提供
专利名称：Production of alpha-ABeta
发明人：Denis Drapeau,Yen-Tung Luan,James R.
Mercer,Wenge Wang,Daniel R. Lasko
申请号：US11213317
申请日：20050825
公开号：US2A1
公开日：20060720
摘要：An improved system for large scale production of proteins and/or polypeptides
in cell culture, particularly in media characterized by one or more of: i) a cumulative amino
acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to
cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to
cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic
ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined
cumulative glutamine and cumulative asparagine concentration between about 16 and 36
mM, is provided. The use of such a system allows high levels of protein production and
lessens accumulation of certain undesirable factors such as ammonium and/or lactate.
Additionally, culture methods including a temperature shift, typically including a decrease
in temperature when the culture has reached about 20-80% of it maximal cell density, are
provided. Alternatively or additionally, the present invention provides methods such that,
after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.
申请人：Denis Drapeau,Yen-Tung Luan,James R. Mercer,Wenge Wang,Daniel R. Lasko
地址：Salem NH US,Chelmsford MA US,Derry NH US,North Chelmsford MA
US,Medford MA US
国籍：US,US,US,US,US
更多信息请下载全文后查看