2024年3月19日发(作者：礼琼)

专利内容由知识产权出版社提供

专利名称：Method for purification of alpha-1-

antitrypsin

发明人：Kee, Scott M.,Cook, Paul I.,Smith, James

R.,Kling, Robert,Fowler, Scott A.,Weber,

David

申请号：AU2015200572

申请日：20150206

公开号：AU2015200572A1

公开日：20150226

摘要：#$%^&*##### ABSTRACT A streamlined method

for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as a

Cohn fraction IV, precipitate, is provided. In the method of the invention, contaminating

proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, 5 such

as a dithiol, which does not affect AAT. The destabilized proteins are then preferentially

adsorbed on a solid protein-adsorbing material, without the addition of a salt as a

precipitant. Separation of the solid adsorbent from the solution leaves a purified AAT

solution that is directly suitable for chromatographic purification, without the need for

extensive desalting as in prior art processes. A process incorporating this method, which

provides pharmaceutical grade AAT in 10 high yield on a commercial scale, is also

described.

申请人：CSL Behring L.L.C.

代理人：Davies Collison Cave

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2024年3月19日发(作者：礼琼)

专利内容由知识产权出版社提供

专利名称：Method for purification of alpha-1-

antitrypsin

发明人：Kee, Scott M.,Cook, Paul I.,Smith, James

R.,Kling, Robert,Fowler, Scott A.,Weber,

David

申请号：AU2015200572

申请日：20150206

公开号：AU2015200572A1

公开日：20150226

摘要：#$%^&*##### ABSTRACT A streamlined method

for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as a

Cohn fraction IV, precipitate, is provided. In the method of the invention, contaminating

proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, 5 such

as a dithiol, which does not affect AAT. The destabilized proteins are then preferentially

adsorbed on a solid protein-adsorbing material, without the addition of a salt as a

precipitant. Separation of the solid adsorbent from the solution leaves a purified AAT

solution that is directly suitable for chromatographic purification, without the need for

extensive desalting as in prior art processes. A process incorporating this method, which

provides pharmaceutical grade AAT in 10 high yield on a commercial scale, is also

described.

申请人：CSL Behring L.L.C.

代理人：Davies Collison Cave

更多信息请下载全文后查看