2024年3月22日发(作者：计翠巧)
各种激发光和吸收光波长
各样激发光和汲取光波长
此刻发现一个更全的相关激发光和发射光的波长图谱。 这对 CONFOCAL去做多重染色时是必不
行少的参照资料。
所有文件
Excitation and Emission Wavelengths of Fluorophores
Tavi's "FluoroTable": Common fluorophores
(from )
Fluorophore
1,5 IAEDANS
1,8-ANS
4-Methylumbelliferone
5-carboxy-2,7-dichlorofluorescein
5-Carboxyfluorescein (5-FAM)
5-Carboxynapthofluorescein (pH 10)
5-Carboxytetramethylrhodamine (5-TAMRA)
5-FAM (5-Carboxyfluorescein)
5-HAT (Hydroxy Tryptamine)
5-Hydroxy Tryptamine (HAT)
5-ROX (carboxy-X-rhodamine)
Absorption
Emission
Other info
336
372
385
504
492
512/598
542
492
370-415
370-415
578
567
548
542
518
518
520
351
546
360
412, 430
344
540
412, 430
502
460
440-480
455-600
470
436
355-425
490
480
502
529
518
563/668
568
518
520-540
520-540
604
591
552
568
543
543
548
430
647
449, 455
471, 474
445
630
471, 474
526
650
520-650
560-680
550
520
460
466
Ratio Dye, pH
5-TAMRA (5-Carboxytetramethylrhodamine)
6-Carboxyrhodamine 6G
6-CR 6G
6-JOE
7-Amino-4-methylcoumarin
7-Aminoactinomycin D (7-AAD)
7-Hydroxy-4-methylcoumarin
9-Amino-6-chloro-2-methoxyacridine
ABQ
Acid Fuchsin
ACMA (9-Amino-6-chloro-2-methoxyacridine)
Acridine Orange + DNA
Acridine Orange + RNA
Acridine Orange, both DNA & RNA
Acridine Red
Acridine Yellow
Acriflavin
Acriflavin Feulgen SITSA
Aequorin (Photoprotein)
AFPs - AutoFluorescent Protein - (Quantum
Biotechnologies) see sgGFP, sgBFP
Alexa Fluor 350
Photoprotein
346
342
442
441
各种激发光和吸收光波长
Alexa Fluor 430
Alexa Fluor 488
Alexa Fluor 532
Alexa Fluor 546
Alexa Fluor 568
Alexa Fluor 594
Alexa Fluor 633
Alexa Fluor 647
Alexa Fluor 660
Alexa Fluor 680
Alizarin Complexon
431
495, 492
531, 532
556, 557
577, 578
590, 594
632
647
668
679
530-560,
580
530-560
630, 645
345
345
347
353
555
346
350
345
347
540
519, 520
553, 554
572, 573
603
617, 618
650
666
698
702
580,
624-645
580
655, 660
445
425
444
442
655
442
445
425
444
600
446
655, 660
755
520/530 Ratio Dye, Zn2+
505
585
540
595
480
490
560
591
550
580
515
395
520,528
520
550
447, 520
Alizarin Red
Allophycocyanin (APC)
AMC, AMCA-S
AMCA (Aminomethylcoumarin)
AMCA-X
Aminoactinomycin D
Aminocoumarin
Aminomethylcoumarin (AMCA)
Anilin Blue
Anthrocyl stearate
APC (Allophycocyanin)
APC-Cy7
APTRA-BTC = Ratio Dye, Zn2+
APTS
Astrazon Brilliant Red 4G
Astrazon Orange R
Astrazon Red 6B
Astrazon Yellow 7 GLL
Atabrine
ATTO-TAG CBQCA
ATTO-TAG FQ
Auramine
Aurophosphine G
Aurophosphine
BAO 9(Bisaminophenyloxadiazole)
BCECF (high pH)
BCECF (low pH)
Berberine Sulphate
Beta Lactamase
360-381
630, 645
625-650
466/380
424
500
470
520
450
436
465
486
460
450
450-490
365
492, 503
482
430
409
2024年3月22日发(作者：计翠巧)
各种激发光和吸收光波长
各样激发光和汲取光波长
此刻发现一个更全的相关激发光和发射光的波长图谱。 这对 CONFOCAL去做多重染色时是必不
行少的参照资料。
所有文件
Excitation and Emission Wavelengths of Fluorophores
Tavi's "FluoroTable": Common fluorophores
(from )
Fluorophore
1,5 IAEDANS
1,8-ANS
4-Methylumbelliferone
5-carboxy-2,7-dichlorofluorescein
5-Carboxyfluorescein (5-FAM)
5-Carboxynapthofluorescein (pH 10)
5-Carboxytetramethylrhodamine (5-TAMRA)
5-FAM (5-Carboxyfluorescein)
5-HAT (Hydroxy Tryptamine)
5-Hydroxy Tryptamine (HAT)
5-ROX (carboxy-X-rhodamine)
Absorption
Emission
Other info
336
372
385
504
492
512/598
542
492
370-415
370-415
578
567
548
542
518
518
520
351
546
360
412, 430
344
540
412, 430
502
460
440-480
455-600
470
436
355-425
490
480
502
529
518
563/668
568
518
520-540
520-540
604
591
552
568
543
543
548
430
647
449, 455
471, 474
445
630
471, 474
526
650
520-650
560-680
550
520
460
466
Ratio Dye, pH
5-TAMRA (5-Carboxytetramethylrhodamine)
6-Carboxyrhodamine 6G
6-CR 6G
6-JOE
7-Amino-4-methylcoumarin
7-Aminoactinomycin D (7-AAD)
7-Hydroxy-4-methylcoumarin
9-Amino-6-chloro-2-methoxyacridine
ABQ
Acid Fuchsin
ACMA (9-Amino-6-chloro-2-methoxyacridine)
Acridine Orange + DNA
Acridine Orange + RNA
Acridine Orange, both DNA & RNA
Acridine Red
Acridine Yellow
Acriflavin
Acriflavin Feulgen SITSA
Aequorin (Photoprotein)
AFPs - AutoFluorescent Protein - (Quantum
Biotechnologies) see sgGFP, sgBFP
Alexa Fluor 350
Photoprotein
346
342
442
441
各种激发光和吸收光波长
Alexa Fluor 430
Alexa Fluor 488
Alexa Fluor 532
Alexa Fluor 546
Alexa Fluor 568
Alexa Fluor 594
Alexa Fluor 633
Alexa Fluor 647
Alexa Fluor 660
Alexa Fluor 680
Alizarin Complexon
431
495, 492
531, 532
556, 557
577, 578
590, 594
632
647
668
679
530-560,
580
530-560
630, 645
345
345
347
353
555
346
350
345
347
540
519, 520
553, 554
572, 573
603
617, 618
650
666
698
702
580,
624-645
580
655, 660
445
425
444
442
655
442
445
425
444
600
446
655, 660
755
520/530 Ratio Dye, Zn2+
505
585
540
595
480
490
560
591
550
580
515
395
520,528
520
550
447, 520
Alizarin Red
Allophycocyanin (APC)
AMC, AMCA-S
AMCA (Aminomethylcoumarin)
AMCA-X
Aminoactinomycin D
Aminocoumarin
Aminomethylcoumarin (AMCA)
Anilin Blue
Anthrocyl stearate
APC (Allophycocyanin)
APC-Cy7
APTRA-BTC = Ratio Dye, Zn2+
APTS
Astrazon Brilliant Red 4G
Astrazon Orange R
Astrazon Red 6B
Astrazon Yellow 7 GLL
Atabrine
ATTO-TAG CBQCA
ATTO-TAG FQ
Auramine
Aurophosphine G
Aurophosphine
BAO 9(Bisaminophenyloxadiazole)
BCECF (high pH)
BCECF (low pH)
Berberine Sulphate
Beta Lactamase
360-381
630, 645
625-650
466/380
424
500
470
520
450
436
465
486
460
450
450-490
365
492, 503
482
430
409