2024年3月27日发(作者：颛孙访梦)
EnzAc
yme
c I
Ba
m
H I
BgCl
l II
a I
Ec
o
R I
Ec
o
R
V
HiHi
nc nd
II
III
KpNc
n I
o I
N
de
I
NoPsPvSaSa
t I
t I
u I
c I
l I
S
m
a I
SpSpXbXh
e I
h I
a I
o I
10
Sup
plie10
d
er
×
BuffM
10
×K
10 10 10 10 10 10
××××××
H
M
H
H
M
M
10
×L
10 ×H 10
10
×L
10
10 ×T 10 10
×+B×
H
S
A
×
M
H
×K 10 +B10 ×K
+B×SA ×+B
S
A
H
+TrH
S
ito
n
1
0.
Acc
I
-
5
K
1
× ×T
1
×
1
×
0.
5
×
K
1
×
1
×
1
×
×0.5
M 1 × K
+B×T
+B
S
A
SA
1
×
M
0.
5
×
K
1
×
M
1.
5
×
T
1
×T 1
+B×
S
A
0.
0.
Bam5
H I
×
K
-
1 1 1 1
0.
5
K
1
0.
5
K
1
×K
+B
S
A
0.5
1 × K 1
SA
1
0.
5
1.
5
×
5
× 1 1
× T ×
S
A
1
Bgl
II
1
×
1
× -
1
×
1
×
1
×
2
×
1
×
1
×
×
K
+B
S
A
1
1
Cla I
×
1
×
1
× -
1
×
1
×
1
×
1
×
1
×
×
K
+B
S
A
1
×
1
×
1
×
1
× -
1
×
1
×
1
×
1
×
1
×
K
+B
1
×
H
1 ×
H
+B
SA
1
×
1
×
1
×
1
×
1
×
H
1 ×
H
+B
SA
1
×
1
×
1
×
1
×
1
×
H
1 ×
H
+B
SA
1
×
1
×
0.
5
×
K
1
×
H
1
×
T
+B
S
A
1
×
T
+B
S
A
1
×
T
+B
1
×
1
×
1
×
1
×
1
×
1
×
M
A
10
×
M
+B
S
A
10
×
H
0.
5
×
K
1
×
1
×
M
M
M
M
M
M
M
0.
5
×
K
1
×
K
×K
×K
×K
×K
× ×K
× ×K
+B×K
×K
×
K
T
+BK
K
2
×
1
×
T
K
H
H
H
K
K
T
H
K
H
H
T
H
1
×
1
×
M
K
H
H
H
M
M
M
H
K
M
H
M
H
M
H
Eco
R I
1
×
1
×
M
K
H
H
H
M
M
M
H
K
M
H
H
H
M
H
S
A
1
0.
Eco
R V
5
×
K
1
×
1
×
1
×
1
× -
2
×
1
×
0.×
5 K
× +B
K
S
A
1
Hinc
II
1
×
M
0.
5
×
K
2
×
1
×
1
×
2
× -
1
×
1
×
×
M
+B
S
A
1
Hind
III
1
×
1
×
1
×
1
×
1
×
1
×
1
× -
1
×
M
×
K
+B
S
A
0.
1
×
M
0.
5
×
K
1
×
1
×
1
×
0.
5
×
K
5
1
×
1
× -
× 1
K ×
+BT
S
A
1
×
Nco M
I
+B
S
A
1
×
1
×
1
×
1
×
1
×
1
×
0.
5
×
K
+B
S
A
1
Nde
I
1
×
1
×
1
×
1
×
1
×
1
×
1
×
1
×
1
×
×
K
+B
S
A
-
1 ×
H
+B
SA
1
×
1
×
1
×
1
×
-
1 1 1
×
0.
5
×
0.5
× K
+B
SA
1
×
M
0.
5
×
K
1
1.
5
×
× T
L
+B
S
A
1.
5
×
1
×
K
0.5
× K
+B
SA
1
×
1
×
1
×
1.
5
1
×
T
0.5
× K
+B
SA
1
×
M
0.
5
×
K
1
×
M
1.
5
1
×
H
1 ×
H
+B
SA
1
×
1
×
0.
5
×
K
1
S
A
0.
5
× 1 1
×
2
×
1
× × K ×
S
A
1
×
T
1
×
2
×
1
×
1
×
K
H
H
H
T
K
H
K
H
+BH
H
T
H
K
M
M
T
M
M
× +B
K
S
A
1
×
T
M
T
M
M
1
×
1
×
1
×
1
×
M
K
K
M
M
K
M
M
K
M
× +B
K
S
A
1
×
T
+B
S
A
M
K
M
M
Kpn
I
1
×
M
0.
5
×
K
1
×
1
×
T
M
M
M
M
M
M
1
×
K
+B
SA
1
×
1
×
1
×
1
×
1
× × 0.5 ×
K K K K M K
+B+B+B+B+B+B
SSSSSS
A
A
A
A
A
A
K × K K K
+B+B+B+B
S
A
SA
SS
A
A
K T
+B+B
SS
A
A
T K K M K
+B+B+B+B+B
SSSSS
A
A
A
A
A
1
×
T
+B
S
A
1
×
1
×
1
×
1
×
T
K
H
H
H
H
T
K
T
H
K
T
H
H
H
T
H
2024年3月27日发(作者：颛孙访梦)
EnzAc
yme
c I
Ba
m
H I
BgCl
l II
a I
Ec
o
R I
Ec
o
R
V
HiHi
nc nd
II
III
KpNc
n I
o I
N
de
I
NoPsPvSaSa
t I
t I
u I
c I
l I
S
m
a I
SpSpXbXh
e I
h I
a I
o I
10
Sup
plie10
d
er
×
BuffM
10
×K
10 10 10 10 10 10
××××××
H
M
H
H
M
M
10
×L
10 ×H 10
10
×L
10
10 ×T 10 10
×+B×
H
S
A
×
M
H
×K 10 +B10 ×K
+B×SA ×+B
S
A
H
+TrH
S
ito
n
1
0.
Acc
I
-
5
K
1
× ×T
1
×
1
×
0.
5
×
K
1
×
1
×
1
×
×0.5
M 1 × K
+B×T
+B
S
A
SA
1
×
M
0.
5
×
K
1
×
M
1.
5
×
T
1
×T 1
+B×
S
A
0.
0.
Bam5
H I
×
K
-
1 1 1 1
0.
5
K
1
0.
5
K
1
×K
+B
S
A
0.5
1 × K 1
SA
1
0.
5
1.
5
×
5
× 1 1
× T ×
S
A
1
Bgl
II
1
×
1
× -
1
×
1
×
1
×
2
×
1
×
1
×
×
K
+B
S
A
1
1
Cla I
×
1
×
1
× -
1
×
1
×
1
×
1
×
1
×
×
K
+B
S
A
1
×
1
×
1
×
1
× -
1
×
1
×
1
×
1
×
1
×
K
+B
1
×
H
1 ×
H
+B
SA
1
×
1
×
1
×
1
×
1
×
H
1 ×
H
+B
SA
1
×
1
×
1
×
1
×
1
×
H
1 ×
H
+B
SA
1
×
1
×
0.
5
×
K
1
×
H
1
×
T
+B
S
A
1
×
T
+B
S
A
1
×
T
+B
1
×
1
×
1
×
1
×
1
×
1
×
M
A
10
×
M
+B
S
A
10
×
H
0.
5
×
K
1
×
1
×
M
M
M
M
M
M
M
0.
5
×
K
1
×
K
×K
×K
×K
×K
× ×K
× ×K
+B×K
×K
×
K
T
+BK
K
2
×
1
×
T
K
H
H
H
K
K
T
H
K
H
H
T
H
1
×
1
×
M
K
H
H
H
M
M
M
H
K
M
H
M
H
M
H
Eco
R I
1
×
1
×
M
K
H
H
H
M
M
M
H
K
M
H
H
H
M
H
S
A
1
0.
Eco
R V
5
×
K
1
×
1
×
1
×
1
× -
2
×
1
×
0.×
5 K
× +B
K
S
A
1
Hinc
II
1
×
M
0.
5
×
K
2
×
1
×
1
×
2
× -
1
×
1
×
×
M
+B
S
A
1
Hind
III
1
×
1
×
1
×
1
×
1
×
1
×
1
× -
1
×
M
×
K
+B
S
A
0.
1
×
M
0.
5
×
K
1
×
1
×
1
×
0.
5
×
K
5
1
×
1
× -
× 1
K ×
+BT
S
A
1
×
Nco M
I
+B
S
A
1
×
1
×
1
×
1
×
1
×
1
×
0.
5
×
K
+B
S
A
1
Nde
I
1
×
1
×
1
×
1
×
1
×
1
×
1
×
1
×
1
×
×
K
+B
S
A
-
1 ×
H
+B
SA
1
×
1
×
1
×
1
×
-
1 1 1
×
0.
5
×
0.5
× K
+B
SA
1
×
M
0.
5
×
K
1
1.
5
×
× T
L
+B
S
A
1.
5
×
1
×
K
0.5
× K
+B
SA
1
×
1
×
1
×
1.
5
1
×
T
0.5
× K
+B
SA
1
×
M
0.
5
×
K
1
×
M
1.
5
1
×
H
1 ×
H
+B
SA
1
×
1
×
0.
5
×
K
1
S
A
0.
5
× 1 1
×
2
×
1
× × K ×
S
A
1
×
T
1
×
2
×
1
×
1
×
K
H
H
H
T
K
H
K
H
+BH
H
T
H
K
M
M
T
M
M
× +B
K
S
A
1
×
T
M
T
M
M
1
×
1
×
1
×
1
×
M
K
K
M
M
K
M
M
K
M
× +B
K
S
A
1
×
T
+B
S
A
M
K
M
M
Kpn
I
1
×
M
0.
5
×
K
1
×
1
×
T
M
M
M
M
M
M
1
×
K
+B
SA
1
×
1
×
1
×
1
×
1
× × 0.5 ×
K K K K M K
+B+B+B+B+B+B
SSSSSS
A
A
A
A
A
A
K × K K K
+B+B+B+B
S
A
SA
SS
A
A
K T
+B+B
SS
A
A
T K K M K
+B+B+B+B+B
SSSSS
A
A
A
A
A
1
×
T
+B
S
A
1
×
1
×
1
×
1
×
T
K
H
H
H
H
T
K
T
H
K
T
H
H
H
T
H