2024年4月2日发(作者：达乐圣)
美国药典（USP）中规定的色谱柱类型
Chromatographic Reagents
The following list of packings (L), phases (G), and supports (S) is intended to be a convenient
reference for the chromatographer. [note—Particle sizes given in this listing are those
generally provided. Where other, usually finer, sizes are required, the individual monograph
specifies the desired particle size. Within any category of packings or phases listed below,
there may be a wide range of columns available. Where it is necessary to define more
specifically the chromatographic conditions, the individual monograph so indicates.]
Change to read:
Packings
L1—Octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 1.5 to 10
µm in diameter, or a monolithic silica rod.
L2—Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has
been bonded to a solid spherical core, 30 to 50 µm in diameter.
L3—Porous silica particles, 3 USP31 to 10 µm in diameter, or a monolithic silica rod.
USP31
L4—Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50
µm in diameter.
L5—Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50 µm
in diameter.
L6—Strong cation-exchange packing–sulfonated fluorocarbon polymer coated on a solid
spherical core, 30 to 50 µm in diameter.
L7—Octylsilane chemically bonded to totally porous silica particles, 1.5 to 10 µm in
diameter, or a monolithic silica rod. USP31
L8—An essentially monomolecular layer of aminopropylsilane chemically bonded to totally
porous silica gel support, 3 to 10 µm in diameter.
L9—Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic
cation-exchange coating, 3 to 10 µm in diameter.
L10—Nitrile groups chemically bonded to porous silica particles, 3 to 10 µm in diameter.
L11—Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 µm in
diameter.
L12—A strong anion-exchange packing made by chemically bonding a quaternary amine to a
solid silica spherical core, 30 to 50 µm in diameter.
L13—Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in
diameter.
L14—Silica gel having a chemically bonded, strongly basic quaternary ammonium
anion-exchange coating, 5 to 10 µm in diameter.
L15—Hexylsilane chemically bonded to totally porous silica particles, 3 to 10 µm in
diameter.
L16—Dimethylsilane chemically bonded to porous silica particles, 5 to 10 µm in
diameter.
L17—Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 µm in diameter.
L18—Amino and cyano groups chemically bonded to porous silica particles, 3 to 10 µm
in diameter.
L19—Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the calcium form, about 9 µm in diameter.
L20—Dihydroxypropane groups chemically bonded to porous silica particles, 5 to 10 µm
in diameter.
L21—A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10 µm in diameter.
L22—A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, about
10 µm in size.
L23—An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with
quaternary ammonium groups, about 10 µm in size.
L24—A semi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups
on the matrix surface, 32 to 63 µm in diameter.
[note—Available as YMC-Pack PVA-SIL manufactured by YMC Co., Ltd. and distributed by
Waters Corp. ().]
L25—Packing having the capacity to separate compounds with a molecular weight range from
100–5000 (as determined by polyethylene oxide), applied to neutral, anionic, and cationic
water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylated
ether (surface contained some residual carboxyl functional groups) was found suitable.
L26—Butyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in
diameter.
L27—Porous silica particles, 30 to 50 µm in diameter.
L28—A multifunctional support, which consists of a high purity, 100 , spherical silica substrate
that has been bonded with anionic exchanger, amine functionality in addition to a conventional
reversed phase C8 functionality.
L29—Gamma alumina, reverse-phase, low carbon percentage by weight, alumina-based
polybutadiene spherical particles, 5 µm in diameter with a pore volume of 80 .
L30—Ethyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in
diameter.
L31—A hydroxide-selective, strong anion-exchange resin-quaternary amine bonded on latex
particles attached to a core of 8.5-µm macroporous particles having a pore size of 2000
and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene.
L32—A chiral ligand-exchange packing–l-proline copper complex covalently bonded to
irregularly shaped silica particles, 5 to 10 µm in diameter.
L33—Packing having the capacity to separate dextrans by molecular size over a range of
4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability.
[note—Available as TSKgel G4000 SWXL from Tosoh Biosep ().]
L34—Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the lead form, about 9 µm in diameter.
L35—A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular
monolayer bonded phase having a pore size of 150 .
L36—A 3,5-dinitrobenzoyl derivative of l-phenylglycine covalently bonded to 5-µm
aminopropyl silica.
L37—Packing having the capacity to separate proteins by molecular size over a range of
2,000 to 40,000 Da. It is a polymethacrylate gel.
L38—A methacrylate-based size-exclusion packing for water-soluble samples.
L39—A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin.
L40—Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 5 to 20
µm in diameter.
L41—Immobilized 1-acid glycoprotein on spherical silica particles, 5 µm in diameter.
L42—Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5
µm in diameter.
L43—Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 5 to
10 µm in diameter.
L44—A multifunctional support, which consists of a high purity, 60 , spherical silica substrate
that has been bonded with a cationic exchanger, sulfonic acid functionality in addition to a
conventional reversed phase C8 functionality.
L45—Beta cyclodextrin bonded to porous silica particles, 5 to 10 µm in diameter.
L46—Polystyrene/divinylbenzene substrate agglomerated with quaternary amine
functionalized latex beads, about 10 µm in diameter.
L47—High-capacity anion-exchange microporous substrate, fully functionalized with
trimethlyamine groups, 8 µm in diameter.
[note—Available as CarboPac MA1 and distributed by Dionex Corp. ().]
L48—Sulfonated, cross-linked polystyrene with an outer layer of submicron, porous,
anion-exchange microbeads, 15 µm in diameter.
L49—A reversed-phase packing made by coating a thin layer of polybutadiene onto spherical
porous zirconia particles, 3 to 10 µm in diameter.
[note—Available as Zirchrom PBD, manufactured by ZirChrom Separations, Inc., distributed
by Alltech, .]
L50—Multifunction resin with reversed-phase retention and strong anion-exchange
functionalities. The resin consists of ethylvinylbenzene, 55% cross-linked with divinylbenzene
copolymer, 3 to 15 µm in diameter, and a surface area not less than 350 m2 per g.
Substrate is coated with quaternary ammonium functionalized latex particles consisting of
styrene cross-linked with divinylbenzene.
[note—Available as OmniPac PAX-500 and distributed by Dionex Corp. ().]
L51—Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 5
to 10 µm in diameter.
[note—Available as Chiralpak AD from Chiral Technologies, Inc., ().]
L52—A strong cation-exchange resin made of porous silica with sulfopropyl groups, 5 to 10
µm in diameter.
[note—Available as TSK IC SW Cation from Tosoh Biosep ().]
L53—Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with
divinylbenzene copolymer, 3 to 15 µm diameter. Substrate is surface grafted with
carboxylic acid and/or phosphoric acid functionalized monomers. Capacity not less than 500
µEq/column.
[note—Available as IonPac CS14 distributed by Dionex Corp. ().]
L54—A size exclusion medium made of covalent bonding of dextran to highly cross-linked
porous agarose beads, about 13 µm in diameter.
[note—Available as Superdex Peptide HR 10/30 from Amersham Pharmacia Biotech
().]
L55—A strong cation-exchange resin made of porous silica coated with polybutadiene–maleic
acid copolymer, about 5 µm in diameter.
[note—Available as IC-Pak C M/D from Waters Corp. ().]
L56—Propyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in
diameter.
[note—Available as Zorbax SB-C3 from Agilent Technologies (/chem).]
L57—A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5
µm in diameter, with a pore size of 120 .
[note—Available as Ultron ES-OVM from Agilent Technologies (/chem).]
L58—Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the sodium form, about 6 to 30 µm 1S (USP31) in
diameter.
[note—Available as Aminex HPX-87N from Bio-Rad Laboratories, (2000/01 catalog,
#125-0143) .]
L59—Packing having the capacity to separate proteins by molecular weight over the range of
10 to 500 kDa. It is spherical (10 µm), silica-based, and processed to provide hydrophilic
characteristics and pH stability.
[note—Available as TSKgel G3000SW Column (analytical column) and TSKgel Guard (guard
column) from Tosoh Biosep (part numbers 05789 and 05371, respectively)
().]
L60—Spherical, porous silica gel, 10 µm or less in diameter, the surface of which has
been covalently modified with alkyl amide groups and endcapped.
[note—Available as Supelcosil ABZ from Supelco (/supelco).]
L61—A hydroxide selective strong anion-exchange resin consisting of a highly cross-linked
core of 13 µm microporous particles having a pore size less than 10 units and consisting
of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of
85 nm diameter microbeads bonded with alkanol quaternary ammonium ions (6%).
[note—Available as Ion Pac AS-11 and AG-11 from Dionex ().]
L62—C30 silane bonded phase on a fully porous spherical silica, 3 to 15 µm in
diameter.
L63— Glycopeptide teicoplanin linked through multiple covalent bonds to a 100- units
spherical silica.
[Note—Available as Chirobiotic T from Astec ().] 1S (USP31)
美国药典(USP)规定的色谱柱编号见下面，是对应的色谱柱类型。
L1：十八烷基键合多孔硅胶或无机氧化物微粒固定相，
简称C18或ODS
L2：30～50um表面多孔薄壳型键合C18(ODS)固定相
L3：多孔硅胶微粒
即一般的硅胶柱
L4：30～50um表面多孔薄壳型硅胶
L5：30～50um表面多孔薄壳型氧化铝
L6：30～50um实心微球表面包覆磺化碳氟聚合物－强阳离子交换固定相
L7：全多孔硅胶微粒键合C8官能团固定相
简称C8柱
L8：全多孔硅胶微粒键合非交联NH2固定相
简称NH2柱
L9：强酸性阳离子交换基团键合全多孔不规则形硅胶固定相
L10：多孔硅胶微球键合氰基固定相（CN）
简称CN柱
L11：键合苯基多孔硅胶微球固定相
简称苯基柱
L12：无孔微球键合季胺功能团的强阴离子填料
L13：三乙基硅烷化学键合全多孔硅胶微球固定相（C1）
简称C1柱
L14:10um硅胶化学键合强碱性季铵盐阴离子交换固定相
简称SAX柱
L15：已基硅烷化学键合全多孔硅胶微球固定相
简称C6柱
L16：二甲基硅烷化学键合全多孔硅胶微粒固定相
L17：氢型磺化交联苯乙烯-二乙烯基苯共聚物,强阳离子交换树脂
L18: 3～10um全多孔硅胶化学键合胺基（NH2）和氰基（CN）
L19：钙型磺化交联苯乙烯－二乙烯基苯共聚物，强阳离子交换树脂
L20：二羟基丙烷基化学键合多孔硅胶微球固定相（Diol）
简称二醇基柱
L21：刚性苯乙烯－二乙烯基苯共聚物微球
L22：带有磺酸基团的多孔苯乙烯阳离子交换树脂
L23：带有季胺基团的聚甲基丙烯酸甲酯或聚丙烯酸酯多孔离子交换树脂
L24：表面含有大量羟基的半刚性聚乙烯醇亲水凝胶
L25：聚甲基丙烯酸酯树脂交联羟基醚（表面含有残余羧基功能团）树脂。能分离分子量100～
5000MW范围的水溶性中性、阳离子型及阴离子型聚合物（用聚氧乙烯测定）的固定相
L26：丁基硅烷化学键合全多孔硅胶微球固定相
L27：30～50um的全多孔硅胶微粒
L28：多功能载体，100Å的高纯硅胶加以氨基键合以及C8反相键合的官能团
L29: 氧化铝,反相键合,含碳量低,氧化铝基聚丁二稀小球,5um，孔径80Å
L30: 全多孔硅胶键合乙基硅烷固定相
L31: 季胺基改性孔径2000Å的交联苯乙烯和二乙烯基苯(55%)强阴离子交换树脂
L32: L-脯氨酸铜配合物共价键合于不规则形硅胶微粒的配位体的交换手性色谱填料
L33: 能够分离分子量4000～40000MW范围蛋白质分子的球形硅胶固定相,pH稳定性好
L34：铅型磺化交联苯乙烯－二乙烯基苯共聚物强阳离子交换树脂，9um球形
L35：锆稳定的硅胶微球键合二醇基亲水分子单层固定相，孔径150Å
L36: 5um胺丙基硅胶键合L-苯基氨基乙酸－3,5二硝基苯甲酰
L37：适合分离分子量2000～40,000Mw的聚甲基丙烯酸酯凝胶
L38：水溶性甲基丙烯酸酯基质SEC色谱柱
L39：亲水全多孔聚羟基甲基丙烯酸酯色谱柱
L40：Tris 3,5－二甲基苯基氨基甲酸酯纤维素涂覆多孔硅胶微球
L41：球形硅胶表面固定α1酸糖蛋白固定相
L42: C8和C18硅烷化学键合多孔硅胶固定相
L43: 硅胶微球键合五氟代苯基固定相
L44: 多功能固定相,60 Å高纯硅胶基质键合磺酸阳离子交换功能团和C8反相功能团
L45: β-环糊精键合多孔硅胶微球
L46: 季胺基改性苯乙烯-二乙烯基苯聚合物微球
L1 Octadecyl silane chemically bonded to porous silica or ceramic microparticles,3 to
10µ in diameter.
例如： Luna 5µ C18(2) Synergi Hydro-RP Spherical
L2 Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has
been bonded to a solid spherical core, 30 to 50µ in diameter.
L3 Porous silica microparticles, 5 to 10µ in diameter.
例如： Luna 5µ Silica(2) Spherical
L4 Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50µ in
diameter.
L5 Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50µ in
diameter.
L6 Strong cation-exchange packing: sulfonated fluorocarbon polymer coated on a solid
spherical core, 30 to 50µ in diameter.
L7 Octyl silane chemically bonded to totally porous microsilica particles, 3 to 10µ in
diameter.
例如：Luna 5µ C8(2) Spherical
L8 An essentially monomolecular layer of aminopropyl-silane chemically bonded to totally
porous silica gel support, 10µ in diameter.
例如：Luna 10µ NH2 Spherical
L9 10µ irregular totally porous silica gel having a chemically bonded, strongly acidic
cation-exchange coating.
例如：Partisil 10µ SCX Irregular
L10 Nitrile groups chemically bonded to porous silica microparticles, 3 to 10µ in
diameter.
例如：Luna 5µ CN 100Å；Capcell 5µ CN UG Spherical
L11 Phenyl groups chemically bonded to porous silica microparticles, 3 to 10µ in
diameter.
例如：Luna 5µ Phenyl-Hexyl；Synergi Polar-RP；Prodigy 5µ PH-3 Spherical
L12 Strong anion-exchange packing made by chemically bonding a quaternary amine to a
solid silica spherical core, 30 to 50µm in diameter.
L13 Trimethylsilane chemically bonded to porous silica microparticles, 3 to 10µ in
diameter.
例如：Develosil TMS-UG (C1) 130Å Spherical
L14 Silica gel, 10µ in diameter, having a chemically bonded, strongly basic quaternary
ammonium anion-exchange coating. 例如：Spherex 10µ SAX 100Å Spherical
L15 Hexyl silane chemically bonded to totally porous silica particles, 3 to 10µ in
diameter.
例如：PhenoSphere C6 Spherical
L16 Dimethyl silane chemically bonded to totally porous silica particles, 3 to 10µ in
diameter.
例如：Maxsil 5µ RP2 60Å Irregular
L17 Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11µ in diameter.
例如：Rezex RHM Monosaccharide Spherical
L18 Amino and cyano groups chemically bonded to porous silica particles, 5 to 10µ in
diameter.
例如：Partisil 5µ, 10µ PAC Irregular
L19 Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the calcium form, 9µ in diameter.
例如：Rezex RCURezex RCM Spherical
L20 Dihydroxypropane groups chemically bonded to porous silica particles, 3 to 10µ in
diameter.
例如：Spherex 5µ Diol Spherical
L21 A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10µ in diameter.
例如：PolymerX 100Å 5µ, 10µ RP-1 Spherical
L22 A cation exchange resin made of porous polystyrene gel with sulfonic acid groups, about
10µ in size.
例如：Rezex ROA Spherical
L23 An ion exchange resin made of porous polymethacrylate or polyacrylate gel with
quaternary ammonium groups, about 10µ in size. 例如： Shodex IEC QA-825 Spherical
L24 A semi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups on
the matrix surface, 32 to 63µ in diameter.
L25 Packing having the capacity to separate compounds with a MW range from 100 to 5000
daltons (as determined by polyethylene oxide), applied to neutral, anionic, and cationic
water-soluble polymers. A polymethacrylate resin base, crosslinked with poly-hydroxylated
ether (surface contained some residual carboxyl functional groups) was found suitable.
例如：Shodex OHpak SB-802.5 Spherical
L26 Butyl silane chemically bonded to totally porous silica particles, 5 to 10µ in diameter.
例如：PrimeSphere 5µ C4；Jupiter 300Å C4 Spherical
L27 Porous silica particles, 30 to 50µ in diameter.
L28 A multifunctional support, which consists of a high purity, 100Å, spherical silica
substrate that has been bonded with anionic (amine) functionality in addition to a conventional
reversed phase C8 functionality.
L29 Gamma alumina, reversed phase, low carbon percentage by weight, alumina-based
polybutadiene spherical particles, 5µ diameter w/ a pore diameter of 80Å.
L30 Ethyl silane chemically bonded to a totally porous silica particle, 3 to 10µ in
diameter.
例如：Maxsil 5µ RP2 60Å Irregular
L31 A strong anion-exchange resin-quaternary amine bonded on latex particles attached to a
core of 8.5µ macroporous particles having a pore size of 2000Å and consisting of
ethylvinylbenzene cross-linked with 55% divinyl benzene.
L32 A chiral ligand-exchange packing- L-proline copper complex covalently bonded to
irregularly shaped silica particles, 5 to 10µ in diameter.
例如：Nucleosil Chiral-1 Spherical
L33 Packing having the capacity to separate proteins of 4,000 to 400,000 daltons. It is
spherical, silica-based and processed to provide pH stability.
例如：BioSep-SEC-S2000BioSep-SEC-S3000 Spherical
L34 Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the lead form, about 9µ in diameter.
例如：Rezex RPM Monosaccharide Spherical
L35 A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular
monolayer bonded phase having a pore size of 150Å.
例如：(BioSep-SEC-S2000 may be used) Spherical
L36 3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5µ
aminopropyl silica.
例如：Nucleosil Chiral-3 Spherical
L37 Polymethacrylate gel packing having the capacity to separate proteins by molecular size
over a range of 2,000 to 40,000D. 例如：PolySep-GFC-P 3000；Shodex OHpak SB-803HQ
Spherical
L38 Methacrylate-based size-exclusion packing for water-soluble samples.
例如：PolySep-GFC-P 1000；Shodex OHpak SB-802HQ Spherical
L39 Hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin.
例如：PolySep-GFC-P；Shodex OHpak SB-800HQ series；Shodex RSpak DM-614 Spherical
L40 Cellulose tri-3,5-dimethylphenylcarbamate coated porous silica particles, 5µ to
20µ in diameter.
L41 Immobilized α-acid glycoprotein on spherical silica particles,5µ in diameter.
例如：Chiral-AGP Spherical
L42 Octylsilane and octadecylsilane groups chemically bonded to porous silica particles,
5µ in diameter.
L43 Pentafluorophenyl groups chemically bonded to silica particles, 5 to 10µ in diameter.
例如：Curosil-PFP Spherical
L44 A multifunctional support, which consists of a high purity, 60Å, spherical silica
substrate that has been bonded with cationic exchanger,sulfonic acid functionality in addition
to a conventional reversed phase C8 functionality.
L45 Beta c
2024年4月2日发(作者：达乐圣)
美国药典（USP）中规定的色谱柱类型
Chromatographic Reagents
The following list of packings (L), phases (G), and supports (S) is intended to be a convenient
reference for the chromatographer. [note—Particle sizes given in this listing are those
generally provided. Where other, usually finer, sizes are required, the individual monograph
specifies the desired particle size. Within any category of packings or phases listed below,
there may be a wide range of columns available. Where it is necessary to define more
specifically the chromatographic conditions, the individual monograph so indicates.]
Change to read:
Packings
L1—Octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 1.5 to 10
µm in diameter, or a monolithic silica rod.
L2—Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has
been bonded to a solid spherical core, 30 to 50 µm in diameter.
L3—Porous silica particles, 3 USP31 to 10 µm in diameter, or a monolithic silica rod.
USP31
L4—Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50
µm in diameter.
L5—Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50 µm
in diameter.
L6—Strong cation-exchange packing–sulfonated fluorocarbon polymer coated on a solid
spherical core, 30 to 50 µm in diameter.
L7—Octylsilane chemically bonded to totally porous silica particles, 1.5 to 10 µm in
diameter, or a monolithic silica rod. USP31
L8—An essentially monomolecular layer of aminopropylsilane chemically bonded to totally
porous silica gel support, 3 to 10 µm in diameter.
L9—Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic
cation-exchange coating, 3 to 10 µm in diameter.
L10—Nitrile groups chemically bonded to porous silica particles, 3 to 10 µm in diameter.
L11—Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 µm in
diameter.
L12—A strong anion-exchange packing made by chemically bonding a quaternary amine to a
solid silica spherical core, 30 to 50 µm in diameter.
L13—Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in
diameter.
L14—Silica gel having a chemically bonded, strongly basic quaternary ammonium
anion-exchange coating, 5 to 10 µm in diameter.
L15—Hexylsilane chemically bonded to totally porous silica particles, 3 to 10 µm in
diameter.
L16—Dimethylsilane chemically bonded to porous silica particles, 5 to 10 µm in
diameter.
L17—Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 µm in diameter.
L18—Amino and cyano groups chemically bonded to porous silica particles, 3 to 10 µm
in diameter.
L19—Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the calcium form, about 9 µm in diameter.
L20—Dihydroxypropane groups chemically bonded to porous silica particles, 5 to 10 µm
in diameter.
L21—A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10 µm in diameter.
L22—A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, about
10 µm in size.
L23—An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with
quaternary ammonium groups, about 10 µm in size.
L24—A semi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups
on the matrix surface, 32 to 63 µm in diameter.
[note—Available as YMC-Pack PVA-SIL manufactured by YMC Co., Ltd. and distributed by
Waters Corp. ().]
L25—Packing having the capacity to separate compounds with a molecular weight range from
100–5000 (as determined by polyethylene oxide), applied to neutral, anionic, and cationic
water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylated
ether (surface contained some residual carboxyl functional groups) was found suitable.
L26—Butyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in
diameter.
L27—Porous silica particles, 30 to 50 µm in diameter.
L28—A multifunctional support, which consists of a high purity, 100 , spherical silica substrate
that has been bonded with anionic exchanger, amine functionality in addition to a conventional
reversed phase C8 functionality.
L29—Gamma alumina, reverse-phase, low carbon percentage by weight, alumina-based
polybutadiene spherical particles, 5 µm in diameter with a pore volume of 80 .
L30—Ethyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in
diameter.
L31—A hydroxide-selective, strong anion-exchange resin-quaternary amine bonded on latex
particles attached to a core of 8.5-µm macroporous particles having a pore size of 2000
and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene.
L32—A chiral ligand-exchange packing–l-proline copper complex covalently bonded to
irregularly shaped silica particles, 5 to 10 µm in diameter.
L33—Packing having the capacity to separate dextrans by molecular size over a range of
4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability.
[note—Available as TSKgel G4000 SWXL from Tosoh Biosep ().]
L34—Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the lead form, about 9 µm in diameter.
L35—A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular
monolayer bonded phase having a pore size of 150 .
L36—A 3,5-dinitrobenzoyl derivative of l-phenylglycine covalently bonded to 5-µm
aminopropyl silica.
L37—Packing having the capacity to separate proteins by molecular size over a range of
2,000 to 40,000 Da. It is a polymethacrylate gel.
L38—A methacrylate-based size-exclusion packing for water-soluble samples.
L39—A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin.
L40—Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 5 to 20
µm in diameter.
L41—Immobilized 1-acid glycoprotein on spherical silica particles, 5 µm in diameter.
L42—Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5
µm in diameter.
L43—Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 5 to
10 µm in diameter.
L44—A multifunctional support, which consists of a high purity, 60 , spherical silica substrate
that has been bonded with a cationic exchanger, sulfonic acid functionality in addition to a
conventional reversed phase C8 functionality.
L45—Beta cyclodextrin bonded to porous silica particles, 5 to 10 µm in diameter.
L46—Polystyrene/divinylbenzene substrate agglomerated with quaternary amine
functionalized latex beads, about 10 µm in diameter.
L47—High-capacity anion-exchange microporous substrate, fully functionalized with
trimethlyamine groups, 8 µm in diameter.
[note—Available as CarboPac MA1 and distributed by Dionex Corp. ().]
L48—Sulfonated, cross-linked polystyrene with an outer layer of submicron, porous,
anion-exchange microbeads, 15 µm in diameter.
L49—A reversed-phase packing made by coating a thin layer of polybutadiene onto spherical
porous zirconia particles, 3 to 10 µm in diameter.
[note—Available as Zirchrom PBD, manufactured by ZirChrom Separations, Inc., distributed
by Alltech, .]
L50—Multifunction resin with reversed-phase retention and strong anion-exchange
functionalities. The resin consists of ethylvinylbenzene, 55% cross-linked with divinylbenzene
copolymer, 3 to 15 µm in diameter, and a surface area not less than 350 m2 per g.
Substrate is coated with quaternary ammonium functionalized latex particles consisting of
styrene cross-linked with divinylbenzene.
[note—Available as OmniPac PAX-500 and distributed by Dionex Corp. ().]
L51—Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 5
to 10 µm in diameter.
[note—Available as Chiralpak AD from Chiral Technologies, Inc., ().]
L52—A strong cation-exchange resin made of porous silica with sulfopropyl groups, 5 to 10
µm in diameter.
[note—Available as TSK IC SW Cation from Tosoh Biosep ().]
L53—Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with
divinylbenzene copolymer, 3 to 15 µm diameter. Substrate is surface grafted with
carboxylic acid and/or phosphoric acid functionalized monomers. Capacity not less than 500
µEq/column.
[note—Available as IonPac CS14 distributed by Dionex Corp. ().]
L54—A size exclusion medium made of covalent bonding of dextran to highly cross-linked
porous agarose beads, about 13 µm in diameter.
[note—Available as Superdex Peptide HR 10/30 from Amersham Pharmacia Biotech
().]
L55—A strong cation-exchange resin made of porous silica coated with polybutadiene–maleic
acid copolymer, about 5 µm in diameter.
[note—Available as IC-Pak C M/D from Waters Corp. ().]
L56—Propyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in
diameter.
[note—Available as Zorbax SB-C3 from Agilent Technologies (/chem).]
L57—A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5
µm in diameter, with a pore size of 120 .
[note—Available as Ultron ES-OVM from Agilent Technologies (/chem).]
L58—Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the sodium form, about 6 to 30 µm 1S (USP31) in
diameter.
[note—Available as Aminex HPX-87N from Bio-Rad Laboratories, (2000/01 catalog,
#125-0143) .]
L59—Packing having the capacity to separate proteins by molecular weight over the range of
10 to 500 kDa. It is spherical (10 µm), silica-based, and processed to provide hydrophilic
characteristics and pH stability.
[note—Available as TSKgel G3000SW Column (analytical column) and TSKgel Guard (guard
column) from Tosoh Biosep (part numbers 05789 and 05371, respectively)
().]
L60—Spherical, porous silica gel, 10 µm or less in diameter, the surface of which has
been covalently modified with alkyl amide groups and endcapped.
[note—Available as Supelcosil ABZ from Supelco (/supelco).]
L61—A hydroxide selective strong anion-exchange resin consisting of a highly cross-linked
core of 13 µm microporous particles having a pore size less than 10 units and consisting
of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of
85 nm diameter microbeads bonded with alkanol quaternary ammonium ions (6%).
[note—Available as Ion Pac AS-11 and AG-11 from Dionex ().]
L62—C30 silane bonded phase on a fully porous spherical silica, 3 to 15 µm in
diameter.
L63— Glycopeptide teicoplanin linked through multiple covalent bonds to a 100- units
spherical silica.
[Note—Available as Chirobiotic T from Astec ().] 1S (USP31)
美国药典(USP)规定的色谱柱编号见下面，是对应的色谱柱类型。
L1：十八烷基键合多孔硅胶或无机氧化物微粒固定相，
简称C18或ODS
L2：30～50um表面多孔薄壳型键合C18(ODS)固定相
L3：多孔硅胶微粒
即一般的硅胶柱
L4：30～50um表面多孔薄壳型硅胶
L5：30～50um表面多孔薄壳型氧化铝
L6：30～50um实心微球表面包覆磺化碳氟聚合物－强阳离子交换固定相
L7：全多孔硅胶微粒键合C8官能团固定相
简称C8柱
L8：全多孔硅胶微粒键合非交联NH2固定相
简称NH2柱
L9：强酸性阳离子交换基团键合全多孔不规则形硅胶固定相
L10：多孔硅胶微球键合氰基固定相（CN）
简称CN柱
L11：键合苯基多孔硅胶微球固定相
简称苯基柱
L12：无孔微球键合季胺功能团的强阴离子填料
L13：三乙基硅烷化学键合全多孔硅胶微球固定相（C1）
简称C1柱
L14:10um硅胶化学键合强碱性季铵盐阴离子交换固定相
简称SAX柱
L15：已基硅烷化学键合全多孔硅胶微球固定相
简称C6柱
L16：二甲基硅烷化学键合全多孔硅胶微粒固定相
L17：氢型磺化交联苯乙烯-二乙烯基苯共聚物,强阳离子交换树脂
L18: 3～10um全多孔硅胶化学键合胺基（NH2）和氰基（CN）
L19：钙型磺化交联苯乙烯－二乙烯基苯共聚物，强阳离子交换树脂
L20：二羟基丙烷基化学键合多孔硅胶微球固定相（Diol）
简称二醇基柱
L21：刚性苯乙烯－二乙烯基苯共聚物微球
L22：带有磺酸基团的多孔苯乙烯阳离子交换树脂
L23：带有季胺基团的聚甲基丙烯酸甲酯或聚丙烯酸酯多孔离子交换树脂
L24：表面含有大量羟基的半刚性聚乙烯醇亲水凝胶
L25：聚甲基丙烯酸酯树脂交联羟基醚（表面含有残余羧基功能团）树脂。能分离分子量100～
5000MW范围的水溶性中性、阳离子型及阴离子型聚合物（用聚氧乙烯测定）的固定相
L26：丁基硅烷化学键合全多孔硅胶微球固定相
L27：30～50um的全多孔硅胶微粒
L28：多功能载体，100Å的高纯硅胶加以氨基键合以及C8反相键合的官能团
L29: 氧化铝,反相键合,含碳量低,氧化铝基聚丁二稀小球,5um，孔径80Å
L30: 全多孔硅胶键合乙基硅烷固定相
L31: 季胺基改性孔径2000Å的交联苯乙烯和二乙烯基苯(55%)强阴离子交换树脂
L32: L-脯氨酸铜配合物共价键合于不规则形硅胶微粒的配位体的交换手性色谱填料
L33: 能够分离分子量4000～40000MW范围蛋白质分子的球形硅胶固定相,pH稳定性好
L34：铅型磺化交联苯乙烯－二乙烯基苯共聚物强阳离子交换树脂，9um球形
L35：锆稳定的硅胶微球键合二醇基亲水分子单层固定相，孔径150Å
L36: 5um胺丙基硅胶键合L-苯基氨基乙酸－3,5二硝基苯甲酰
L37：适合分离分子量2000～40,000Mw的聚甲基丙烯酸酯凝胶
L38：水溶性甲基丙烯酸酯基质SEC色谱柱
L39：亲水全多孔聚羟基甲基丙烯酸酯色谱柱
L40：Tris 3,5－二甲基苯基氨基甲酸酯纤维素涂覆多孔硅胶微球
L41：球形硅胶表面固定α1酸糖蛋白固定相
L42: C8和C18硅烷化学键合多孔硅胶固定相
L43: 硅胶微球键合五氟代苯基固定相
L44: 多功能固定相,60 Å高纯硅胶基质键合磺酸阳离子交换功能团和C8反相功能团
L45: β-环糊精键合多孔硅胶微球
L46: 季胺基改性苯乙烯-二乙烯基苯聚合物微球
L1 Octadecyl silane chemically bonded to porous silica or ceramic microparticles,3 to
10µ in diameter.
例如： Luna 5µ C18(2) Synergi Hydro-RP Spherical
L2 Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has
been bonded to a solid spherical core, 30 to 50µ in diameter.
L3 Porous silica microparticles, 5 to 10µ in diameter.
例如： Luna 5µ Silica(2) Spherical
L4 Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50µ in
diameter.
L5 Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50µ in
diameter.
L6 Strong cation-exchange packing: sulfonated fluorocarbon polymer coated on a solid
spherical core, 30 to 50µ in diameter.
L7 Octyl silane chemically bonded to totally porous microsilica particles, 3 to 10µ in
diameter.
例如：Luna 5µ C8(2) Spherical
L8 An essentially monomolecular layer of aminopropyl-silane chemically bonded to totally
porous silica gel support, 10µ in diameter.
例如：Luna 10µ NH2 Spherical
L9 10µ irregular totally porous silica gel having a chemically bonded, strongly acidic
cation-exchange coating.
例如：Partisil 10µ SCX Irregular
L10 Nitrile groups chemically bonded to porous silica microparticles, 3 to 10µ in
diameter.
例如：Luna 5µ CN 100Å；Capcell 5µ CN UG Spherical
L11 Phenyl groups chemically bonded to porous silica microparticles, 3 to 10µ in
diameter.
例如：Luna 5µ Phenyl-Hexyl；Synergi Polar-RP；Prodigy 5µ PH-3 Spherical
L12 Strong anion-exchange packing made by chemically bonding a quaternary amine to a
solid silica spherical core, 30 to 50µm in diameter.
L13 Trimethylsilane chemically bonded to porous silica microparticles, 3 to 10µ in
diameter.
例如：Develosil TMS-UG (C1) 130Å Spherical
L14 Silica gel, 10µ in diameter, having a chemically bonded, strongly basic quaternary
ammonium anion-exchange coating. 例如：Spherex 10µ SAX 100Å Spherical
L15 Hexyl silane chemically bonded to totally porous silica particles, 3 to 10µ in
diameter.
例如：PhenoSphere C6 Spherical
L16 Dimethyl silane chemically bonded to totally porous silica particles, 3 to 10µ in
diameter.
例如：Maxsil 5µ RP2 60Å Irregular
L17 Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11µ in diameter.
例如：Rezex RHM Monosaccharide Spherical
L18 Amino and cyano groups chemically bonded to porous silica particles, 5 to 10µ in
diameter.
例如：Partisil 5µ, 10µ PAC Irregular
L19 Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the calcium form, 9µ in diameter.
例如：Rezex RCURezex RCM Spherical
L20 Dihydroxypropane groups chemically bonded to porous silica particles, 3 to 10µ in
diameter.
例如：Spherex 5µ Diol Spherical
L21 A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10µ in diameter.
例如：PolymerX 100Å 5µ, 10µ RP-1 Spherical
L22 A cation exchange resin made of porous polystyrene gel with sulfonic acid groups, about
10µ in size.
例如：Rezex ROA Spherical
L23 An ion exchange resin made of porous polymethacrylate or polyacrylate gel with
quaternary ammonium groups, about 10µ in size. 例如： Shodex IEC QA-825 Spherical
L24 A semi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups on
the matrix surface, 32 to 63µ in diameter.
L25 Packing having the capacity to separate compounds with a MW range from 100 to 5000
daltons (as determined by polyethylene oxide), applied to neutral, anionic, and cationic
water-soluble polymers. A polymethacrylate resin base, crosslinked with poly-hydroxylated
ether (surface contained some residual carboxyl functional groups) was found suitable.
例如：Shodex OHpak SB-802.5 Spherical
L26 Butyl silane chemically bonded to totally porous silica particles, 5 to 10µ in diameter.
例如：PrimeSphere 5µ C4；Jupiter 300Å C4 Spherical
L27 Porous silica particles, 30 to 50µ in diameter.
L28 A multifunctional support, which consists of a high purity, 100Å, spherical silica
substrate that has been bonded with anionic (amine) functionality in addition to a conventional
reversed phase C8 functionality.
L29 Gamma alumina, reversed phase, low carbon percentage by weight, alumina-based
polybutadiene spherical particles, 5µ diameter w/ a pore diameter of 80Å.
L30 Ethyl silane chemically bonded to a totally porous silica particle, 3 to 10µ in
diameter.
例如：Maxsil 5µ RP2 60Å Irregular
L31 A strong anion-exchange resin-quaternary amine bonded on latex particles attached to a
core of 8.5µ macroporous particles having a pore size of 2000Å and consisting of
ethylvinylbenzene cross-linked with 55% divinyl benzene.
L32 A chiral ligand-exchange packing- L-proline copper complex covalently bonded to
irregularly shaped silica particles, 5 to 10µ in diameter.
例如：Nucleosil Chiral-1 Spherical
L33 Packing having the capacity to separate proteins of 4,000 to 400,000 daltons. It is
spherical, silica-based and processed to provide pH stability.
例如：BioSep-SEC-S2000BioSep-SEC-S3000 Spherical
L34 Strong cation-exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the lead form, about 9µ in diameter.
例如：Rezex RPM Monosaccharide Spherical
L35 A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular
monolayer bonded phase having a pore size of 150Å.
例如：(BioSep-SEC-S2000 may be used) Spherical
L36 3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5µ
aminopropyl silica.
例如：Nucleosil Chiral-3 Spherical
L37 Polymethacrylate gel packing having the capacity to separate proteins by molecular size
over a range of 2,000 to 40,000D. 例如：PolySep-GFC-P 3000；Shodex OHpak SB-803HQ
Spherical
L38 Methacrylate-based size-exclusion packing for water-soluble samples.
例如：PolySep-GFC-P 1000；Shodex OHpak SB-802HQ Spherical
L39 Hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin.
例如：PolySep-GFC-P；Shodex OHpak SB-800HQ series；Shodex RSpak DM-614 Spherical
L40 Cellulose tri-3,5-dimethylphenylcarbamate coated porous silica particles, 5µ to
20µ in diameter.
L41 Immobilized α-acid glycoprotein on spherical silica particles,5µ in diameter.
例如：Chiral-AGP Spherical
L42 Octylsilane and octadecylsilane groups chemically bonded to porous silica particles,
5µ in diameter.
L43 Pentafluorophenyl groups chemically bonded to silica particles, 5 to 10µ in diameter.
例如：Curosil-PFP Spherical
L44 A multifunctional support, which consists of a high purity, 60Å, spherical silica
substrate that has been bonded with cationic exchanger,sulfonic acid functionality in addition
to a conventional reversed phase C8 functionality.
L45 Beta c