2024年3月10日发(作者：赖欣)
NEBuffer Activity Chart for Restriction Enzymes
New England Biolabs provides a color-coded 10X NEBuffer with every restriction endonuclease to ensure 100%
activity. To help select the best conditions for double digests, this chart shows the optimal (supplied) NEBuffer
and approximate activity in the four standard NEBuffers for each enzyme. If BSA is supplied with an enzyme, it
is included in all NEBuffer activity reactions. In addition, the table also shows recommended reaction
temperature, heat inactivation temperature and recommended diluent buffer.
Please click here for additional information about:
Unique NEBuffer Activity | Double Digestion | Heat Inactivation | Activity at 37°C | Diluent Buffers | Time-Saver
Enzymes | High Fidelity (HF) Restriction Enzymes
This chart has been updated to reflect the change of 54 restriction enzymes to NEBuffer 4 on Nov.1, 2008.
Click for the Chart Legend, Icon Description
A | B | C | D | E | F | H | I | K | M | N | P | R | S | T | X | Z |
% Activity in NEBuffers
1
0
10
50
25
10
50
25
50
25
100
25
10
100
50
50
25
100
25
2
50
75
50
50
10
100
50
100
75
50
75
25
100
100
100
50
100
75
3
50
100
10
100
0
50
25
25
100
10
0
10
75
10
50
0
10
100
4
100
25
100
50
100
100
100
100
50
75
100
100
100
100
100
100
100
50
Heat Incuba.
Inactiva- Temp. Diluent
Enzyme
AatII
Acc65I
AccI
AciI
AclI
AcuI
AfeI
AflII
AflIII
AgeI
AhdI
AleI
AluI
AlwI
AlwNI
ApaI
ApaLI
ApeKI
Supplied NEBuffer
NEBuffer 4
NEBuffer 3 + BSA
NEBuffer 4
NEBuffer 3
NEBuffer 4 + BSA
NEBuffer 4 + SAM
NEBuffer 4
NEBuffer 4 + BSA
NEBuffer 3 + BSA
NEBuffer 1
NEBuffer 4 + BSA
NEBuffer 4
NEBuffer 4
NEBuffer 4
NEBuffer 4
NEBuffer 4 + BSA
NEBuffer 4 + BSA
NEBuffer 3
tion °C
65
65
80
65
No
65
65
65
80
65
65
65
65
65
65
65
No
No
°C
37
37
37
37
37
37
37
37
37
37
37
37
37
37
37
25
37
75
A
A
A
A
B
B
A
A
B
C
A
A
B
A
A
A
A
B
ApoI
AscI
AseI
AsiSI
AvaI
AvaII
AvrII
NEBuffer 3 + BSA
NEBuffer 4
NEBuffer 3
NEBuffer 4 + BSA
NEBuffer 4
NEBuffer 4
NEBuffer 4
10
0
NR
50
10
50
100
75
10
75
100
75
75
100
100
10
100
dd
100
10
10
50
75
100
NR
100
100
100
100
80
65
65
80
80
65
80
Heat
50
37
37
37
37
37
37
Incuba.
A
A
B
B
A
A
B
Diluent
A
A
A
A
A
A
B
A
B
A
A
A
C
A
B
B
B
B
A
A
C
B
B
B
B
B
% in % in % in % in
Enzyme top
BaeGI
BaeI
BamHI
BamHI-HF
™
BanI
BanII
BbsI
BbvCI
BbvI
BccI
BceAI
BcgI
BciVI
BclI
BfaI
BfuAI
BfuCI
BglI
BglII
BlpI
BmgBI
BmrI
BmtI
BpmI
Bpu10I
BpuEI
Supplied NEBuffer
NEBuffer 1
NEBuffer 4 + BSA + SAM
NEBuffer 3 + BSA
NEBuffer 4
NEBuffer 4 + BSA
NEBuffer 4
NEBuffer 2
NEBuffer 4
NEBuffer 2
NEBuffer 1 + BSA
NEBuffer 3 + BSA
NEBuffer 3 + SAM
NEBuffer 4
NEBuffer 3
NEBuffer 4
NEBuffer 3
NEBuffer 4 + BSA
NEBuffer 3
NEBuffer 3
NEBuffer 4
NEBuffer 3 + BSA
NEBuffer 2
NEBuffer 2
NEBuffer 3 + BSA
NEBuffer 3
NEBuffer 4 + SAM
B1
100
50
75
100
50
100
100
50
100
100
100
NR
100
50
75
0
100
50
10
50
0
75
25
75
10
50
B2
75
100
B3
10
50
B4
50
100
Inactiva. Temp.
65
65
No
No
65
80
65
80
65
65
65
65
65
No
80
65
80
65
No
No
65
65
65
65
80
65
37
25
37
37
37
37
37
37
37
37
37
37
37
50
37
50
37
37
37
37
37
37
37
37
37
37
100 100
dd
100
50
100
10
100
100
100
50
100
NR
50
10
50
50
25
10
25
10
100
100
dd
0
100
100
100
75
100
75
50
100
NR
100
75
100
10
100
50
10
100
10
100
50
100
25
100
100 100
dd
50
75
50
75
75
100
25
100
100
100
25
100
10
100
25
100
100
10
100
75
25
100
100
10
2024年3月10日发(作者：赖欣)
NEBuffer Activity Chart for Restriction Enzymes
New England Biolabs provides a color-coded 10X NEBuffer with every restriction endonuclease to ensure 100%
activity. To help select the best conditions for double digests, this chart shows the optimal (supplied) NEBuffer
and approximate activity in the four standard NEBuffers for each enzyme. If BSA is supplied with an enzyme, it
is included in all NEBuffer activity reactions. In addition, the table also shows recommended reaction
temperature, heat inactivation temperature and recommended diluent buffer.
Please click here for additional information about:
Unique NEBuffer Activity | Double Digestion | Heat Inactivation | Activity at 37°C | Diluent Buffers | Time-Saver
Enzymes | High Fidelity (HF) Restriction Enzymes
This chart has been updated to reflect the change of 54 restriction enzymes to NEBuffer 4 on Nov.1, 2008.
Click for the Chart Legend, Icon Description
A | B | C | D | E | F | H | I | K | M | N | P | R | S | T | X | Z |
% Activity in NEBuffers
1
0
10
50
25
10
50
25
50
25
100
25
10
100
50
50
25
100
25
2
50
75
50
50
10
100
50
100
75
50
75
25
100
100
100
50
100
75
3
50
100
10
100
0
50
25
25
100
10
0
10
75
10
50
0
10
100
4
100
25
100
50
100
100
100
100
50
75
100
100
100
100
100
100
100
50
Heat Incuba.
Inactiva- Temp. Diluent
Enzyme
AatII
Acc65I
AccI
AciI
AclI
AcuI
AfeI
AflII
AflIII
AgeI
AhdI
AleI
AluI
AlwI
AlwNI
ApaI
ApaLI
ApeKI
Supplied NEBuffer
NEBuffer 4
NEBuffer 3 + BSA
NEBuffer 4
NEBuffer 3
NEBuffer 4 + BSA
NEBuffer 4 + SAM
NEBuffer 4
NEBuffer 4 + BSA
NEBuffer 3 + BSA
NEBuffer 1
NEBuffer 4 + BSA
NEBuffer 4
NEBuffer 4
NEBuffer 4
NEBuffer 4
NEBuffer 4 + BSA
NEBuffer 4 + BSA
NEBuffer 3
tion °C
65
65
80
65
No
65
65
65
80
65
65
65
65
65
65
65
No
No
°C
37
37
37
37
37
37
37
37
37
37
37
37
37
37
37
25
37
75
A
A
A
A
B
B
A
A
B
C
A
A
B
A
A
A
A
B
ApoI
AscI
AseI
AsiSI
AvaI
AvaII
AvrII
NEBuffer 3 + BSA
NEBuffer 4
NEBuffer 3
NEBuffer 4 + BSA
NEBuffer 4
NEBuffer 4
NEBuffer 4
10
0
NR
50
10
50
100
75
10
75
100
75
75
100
100
10
100
dd
100
10
10
50
75
100
NR
100
100
100
100
80
65
65
80
80
65
80
Heat
50
37
37
37
37
37
37
Incuba.
A
A
B
B
A
A
B
Diluent
A
A
A
A
A
A
B
A
B
A
A
A
C
A
B
B
B
B
A
A
C
B
B
B
B
B
% in % in % in % in
Enzyme top
BaeGI
BaeI
BamHI
BamHI-HF
™
BanI
BanII
BbsI
BbvCI
BbvI
BccI
BceAI
BcgI
BciVI
BclI
BfaI
BfuAI
BfuCI
BglI
BglII
BlpI
BmgBI
BmrI
BmtI
BpmI
Bpu10I
BpuEI
Supplied NEBuffer
NEBuffer 1
NEBuffer 4 + BSA + SAM
NEBuffer 3 + BSA
NEBuffer 4
NEBuffer 4 + BSA
NEBuffer 4
NEBuffer 2
NEBuffer 4
NEBuffer 2
NEBuffer 1 + BSA
NEBuffer 3 + BSA
NEBuffer 3 + SAM
NEBuffer 4
NEBuffer 3
NEBuffer 4
NEBuffer 3
NEBuffer 4 + BSA
NEBuffer 3
NEBuffer 3
NEBuffer 4
NEBuffer 3 + BSA
NEBuffer 2
NEBuffer 2
NEBuffer 3 + BSA
NEBuffer 3
NEBuffer 4 + SAM
B1
100
50
75
100
50
100
100
50
100
100
100
NR
100
50
75
0
100
50
10
50
0
75
25
75
10
50
B2
75
100
B3
10
50
B4
50
100
Inactiva. Temp.
65
65
No
No
65
80
65
80
65
65
65
65
65
No
80
65
80
65
No
No
65
65
65
65
80
65
37
25
37
37
37
37
37
37
37
37
37
37
37
50
37
50
37
37
37
37
37
37
37
37
37
37
100 100
dd
100
50
100
10
100
100
100
50
100
NR
50
10
50
50
25
10
25
10
100
100
dd
0
100
100
100
75
100
75
50
100
NR
100
75
100
10
100
50
10
100
10
100
50
100
25
100
100 100
dd
50
75
50
75
75
100
25
100
100
100
25
100
10
100
25
100
100
10
100
75
25
100
100
10