2024年5月22日发(作者：中嘉悦)
Invitrogen中国使用的测序载体和引物
Primer of Vector
Vector
pACT
pACT2
pAS2-1
pB42AD
pBACPAK8
pBK-CMV
PBS(SK/KS)/M13-
pBV220
pCAMBIA
1301(1300)
pCAMBIA 2300
PCANTB5E
pCAT3-enhancer
pcDNA3.0
pcDNA3.1
pcDNA4
pcDNA6
pcDNAII
pCE2.1
pCEP4
pCF-T
pCI
pCI-neo
pCMS-EGFP
pCMV-3Tag-4A
pCMV5
pCMV5-Flag
pCMV-MYC/HA
pCMV-Sport
pCMV-Tag(KAN+)
pCR2.1-TOPO
pCR3.1
pCS2
pDonar
pDONR221
pDrive
pDRIveR(KAN+)
pDsRED1-C1
pDsRED2-C1(KAN+)
pDSRED-N1(KAN+)
pECFP-C
pECFP-N1
pEF/myc/ER
pEGFP-C(KAN+)
pEGFP-N(KAN+)
pENTR/D-
TOPO(KAN+)
pET-*(His)(KAN+)
pET22(a,b,c)(AMP+
)
Primer(F)
T7
GAL4 AD
GAL4 BD
pB42ADF
BAC1
T7
M13F/T7
PBV220F
P1
M13R(-48)
S1/M13R
RVP3
CMV-F/T7
T7
T7/CMV-F
T7/CMV-F(J21025在T7前
T7
M13F
pCEP-F
M13F
T7（17Base）
T7（17Base）
T7
T3 /PFLAG-CMV-F
pCMV5F
pCMV5F
pCMV-F
M13F/T7
T3
M13F/T7
T7
SP6
M13F
M13F
T7
T7
pDsRED-ex-C1-F
pDsRED-ex-C1-F
pEGFP-N-5’
pEGFP-C-5’
pEGFP-N-5’
pEF-F
pEGFP-C-5’
pEGFP-N-5’
M13F
T7
T7
Primer(R)
T3
pACT2-R
pAS2-1.R
pB42ADR
BAC2
T3
T3/M13R
PBV220R
P2
M13F(-47)
S6
此载体无反向引物
SP6/BGH
BGH
BGH
BGH
SP6
M13R
EBV-R
M13R
此载体无反向引物
T3
T3
T7
pCMV5R
pCMV5R
pCMV-R
SP6/M13R
T7
M13R
BGH
T7
M13R
M13R
SP6
SP6
pEGFP-N-3’(距离很近,一般不用)
pEGFP-N-3’
PDSRED-N-R
pECFP-C-3'
pEGFP-N-3’
pCDNA3.1R
pEGFP-C-3'
pEGFP-N-3’
M13R
T7 Ter
T7 Ter
pET28,30,24,49(KA
N+)
pET32(a,b,c)(AMP+
)
pET50(amp+)
pET44(AMP+)
pEYFP-N1
pEYFP-C1
pFLAG-CMV
pGAD424
pGADGH
pGADT7-Rec
pGAPZa-A
pGBKT7(Kana+)
pGEM-T(-Easy)
pGEX-(*)T
pGL2
pGL3
PinpointTM Vector
pIRES
pIRES2-EGFP
pLXSN
pMAL-c2E
pMAL-C2x
pMAL-p2X
PMD18-T
PMD19-T
pPIC9K(AMP+、
ZEO+）
pPICZa
pPROEXHTA
pQE30or40
pRSET
pSilencer3.1-H1
hygro
pSILENCEV2.0-U6
pSK01-T
pSK-CMV(KAN+)
pSP72
pSport1
pSUPER
pTA2
pT-Adv
pTARGET TM
pTO-T7
pTRC99a-c
pTriPLEx2
pTWIN-1
pUC18(19)/118(119
)
pUCm-T
pVAX1
pWSK29
pXT7
T7
T7/
T7/
T7/
pEGFP-N-5’
pEGFP-C-5’
pFLAG-CMV -F
GAL4 AD
GAL4 AD
GAL4 AD/T7
a-FACTOR
T7
M13F/T7
pGEX-4T-5’
GLP1
RVP3
Pinpoint Primer
PIRES-F/T7
pIRES2-EGFP.P5’
pLXSN-F
MalE primer
P5’
MalE primer
M13R(-48)
M13F(-47)
5’AOX/a-factor
5'AOX/PPICZa-F
M13R(-48)
pQE30-F
T7
M13F(-47)
T7
M13F
T7
T7
SP6/M13F(-47)
T7
M13F/T7(优先使用M13F）
M13F /T7
pTarget.F(在T7前面）/T7
T7
pTRC99C-F
5'pTriPLEx2
T7
M13F(-47)
M13F/T7
T7
T7/M13F/M13F(-47)
T7
T7 Ter
T7 Ter
T7TER
T7 Ter
pEGFP-N-3’
pEGFP-C-3'
pFLAG-CMV -R
3'AD
3'AD
3'AD
3'AOX
3'BD
SP6/M13R
pGEX-4T-3’
GLP2
GLP2(RVP4)(没有特别说明用GLP2)
SP6
T3/PIRES-R (J40720)
pIRES2-EGFP.P3’
pLXSN-R
M13F(-47)
P3’
M13F(-47)
M13F(-47)
M13R(-48)
3’AOX
3'AOX
无反向引物
pQE-R:
T7TER
3.0REV
2.0REV
M13R
T3
SP6
T7/M13R
T3
T3/M13R
M13R
pTarget.R
T7TER
pTRC99C-R
T7
T7TER(客户确认再用）
M13R(-48)
M13R
BGH
T3/M13R/M13R(-48)
SP6
pYES2
pLEXA
pLNCX
pRECEIVER
pSHUTTLE-CMV
pSP64/65
pSTBlue-1
pT7Blue(R)
T7
pLEXA-F
pLNCX-F
CMV-F
PSHUTTLE-CMV-F
SP6
T7
T7
pYes2.R
pLEXA-R
pLNCX-R
此载体无反向引物
PSHUTTLE-CMV-R
此载体无反向引物
SP6
M13F(-47)
Invitrogen中国测序通用引物序列
引物名称
M13R
M13F
M13F(-47)
M13R(-48)
M13(-96)
SP6
T7
T7 terminator
T3
pGEX-4T-5'
pGEX-4T-3'
GLp1
GLp2
RVp3
RVp4
pcDNA3.1R
PinPoint primer
pCMV-F
pCMV-R
pTRC99C-F:
pTRC99C-R:
pCEP-F:
EBV-R :
pIRES2-EGFP.P5’:
pIRES2-EGFP.P3’:
3'AD:
CMV -F
S1
S6
5`AOX1
3`AOX1
α-Factor
GAL4 AD
pACT2-R
pB42ADF:
pB42ADR:
pEGFP-N-5’:
pEGFP-N-3’:
pEGFP-C-5’:
pEGFP-C-3' :
PBV220F:
PBV220R:
U6
2.0rev primer:
3.0rev:
5'TriplEx2
序列(5'-3')
CAG GAA ACA GCT ATG ACC
TGT AAA ACG ACG GCC AGT
CGC CAG GGT TTT CCC AGT CAC GAC
AGC GGA TAA CAA TTT CAC ACA GGA
CCC TCA TAG TTA GCG TAA CG
ATT TAG GTG ACA CTA TAG
TAA TAC GAC TCA CTA TAG GG
TGC TAG TTA TTG CTC AGC GG
ATT AAC CCT CAC TAA AGG GA
GGG CTG GCA AGC CAC GTT TGG TG
CCG GGA GCT GCA TGT GTC AGA GG
TGT ATC TTA TGG TAC TGT AAC TG
CTT TAT GTT TTT GGC GTC TTC CA
CTA GCA AAA TAG GCT GTC CC
GAC GAT AGT CAT GCC CCG CG
TAG AAG GCA CAG TCG AGG
CGT GAC GCG GTG CAG GGC G
TCT AAA AGC TGC GGA ATT GT
TCC AAA CTC ATC AAT GTA TC
TTG CGC CGA CAT CAT AAC
CTG CGT TCT GAT TTA ATC TG
AGA GCT CGT TTA GTG AAC CG
GTG GTT TGT CCA AAC TCA TC
GTA GGC GTG TAC GGT GGG AG
AAC GCA CAC CGG CCT TAT TC
AGA TGG TGC ACG ATG CAC AG
CGC AAA TGG GCG GTA GGC GTG
CAA CGT GAA AAA ATT ATT ATT CGC
GTA AAT GAA TTT TCT GTA GTA GG
GAC TGG TTC CAA TTG ACA AGC
GCA AAT GGC ATT CTG ACA TCC
TAC TAT TGC CAG CAT TGC TGC
TAC CAC TAC AAT GGA TG
GTG CAC GAT GCA CAG TTG AA
CCA GCC TCT TGC TGA GTG GAG ATG
AAG CCG ACA ACC TTG ATT GGA G
TGG GAG GTC TAT ATA AGC AGA G
CGT CGC CGT CCA GCT CGA CCA G
CAT GGT CCT GCT GGA GTT CGT G
TAT GGC TGA TTA TGA TCA GT
AAG AAG GGC AGC ATT CAA AG
CTG CGT TCT GAT TTA ATC TG
ATG GAC TAT CAT ATG CTT ACC GTA
AGG CGA TTA AGT TGG GTA
GAG TTA GCT CAC TCA TTA GGC
GAA CGC CAG CAC ATG GAC
CTC CGA GAT CTG GAC GAG C
RVP4
RVP3
pQE30-R
pQE30-F
pEF-F
pDSRED-N1-R
pDsRED-express-C1-
pCMV5R
pCMV5F
P5'
P3'
BAC2
BAC1
3'BD:
pTarget.F
pTarget.R
T7(17base)
pYes2.R
GAL4 BD
pAS2-1.R
pFlag-CMV-F:
pFlag-CMV-R:
pIRES-F:
pIRES.R:
P1
P2
pLEXA-F
pLEXA-R
pLNCX-F
pLNCX-R
pLXSN-F
pLXSN-R
pSHUTTLE-CMV-F
pSHUTTLE-CMV-R
MalE Primer
GAC GAT AGT CAT GCC CCG CG
CTA GCA AAA TAG GCT GTC CC
GTT CTG AGG TCA TTA CTG G
TGA GCG GAT AAC AAT TTC AC
TCA AGC CTC AGA CAG TGG TTC
TGA AGC GCA TGA ACT CCT TG
TCC CAC AAC GAG GAC TAC AC
ATT ATA GAG GAC ACC TAG TC
TTC CAA AAT GTC GTA ATA AC
TGC GTA CTG CGG TGA TCA AC
CTG CAA GGC GAT TAA GTT GG
ACG CAC AGA ATC TAG CGC TT
AAC CAT CTC GCA AAT AAA TA
TAA GAG TCA CTT TAA AAT TTG TAT AC
CCA GGA TTT TCC CAG TCA C
GGC TTT ACA CTT TAT GCT TC
ACA TCC ACT TTG CCT TTC TC
TCG GTT AGA GCG GAT GTG
TCA TCG GAA GAG AGT AG
AAG AGT TAC TCA AGA ACA AGA A
GGT AGG CGT GTA CGG TGG
GCA CTG GAG TGG CAA CTT
CTT ACT GAC ATC CAC TTT GC
CAC TGC ATT CTA GTT GTG GT
CCA GGC TTT ACA CTT TAT GC
GCG ATT AAG TTG GGT AAC GC
CGT CAG CAG AGC TTC ACC AT
TAA AAC CTA AGA GTC ACT TT
AGC TCG TTT AGT GAA CCG TCA GAT CG
ACC TAC AGG TGG GGT CTT TCA TTC CC
CTT GAA CCT CCT CGT TCG AC
GTT GCT GAC TAA TTG AGA TG
GGT CTA TAT AAG CAG AGG TG
GTG GTA TGG CTG ATT ATG ATC AG
GGT CGT CAG ACT GTC GAT GAA GCC
2024年5月22日发(作者：中嘉悦)
Invitrogen中国使用的测序载体和引物
Primer of Vector
Vector
pACT
pACT2
pAS2-1
pB42AD
pBACPAK8
pBK-CMV
PBS(SK/KS)/M13-
pBV220
pCAMBIA
1301(1300)
pCAMBIA 2300
PCANTB5E
pCAT3-enhancer
pcDNA3.0
pcDNA3.1
pcDNA4
pcDNA6
pcDNAII
pCE2.1
pCEP4
pCF-T
pCI
pCI-neo
pCMS-EGFP
pCMV-3Tag-4A
pCMV5
pCMV5-Flag
pCMV-MYC/HA
pCMV-Sport
pCMV-Tag(KAN+)
pCR2.1-TOPO
pCR3.1
pCS2
pDonar
pDONR221
pDrive
pDRIveR(KAN+)
pDsRED1-C1
pDsRED2-C1(KAN+)
pDSRED-N1(KAN+)
pECFP-C
pECFP-N1
pEF/myc/ER
pEGFP-C(KAN+)
pEGFP-N(KAN+)
pENTR/D-
TOPO(KAN+)
pET-*(His)(KAN+)
pET22(a,b,c)(AMP+
)
Primer(F)
T7
GAL4 AD
GAL4 BD
pB42ADF
BAC1
T7
M13F/T7
PBV220F
P1
M13R(-48)
S1/M13R
RVP3
CMV-F/T7
T7
T7/CMV-F
T7/CMV-F(J21025在T7前
T7
M13F
pCEP-F
M13F
T7（17Base）
T7（17Base）
T7
T3 /PFLAG-CMV-F
pCMV5F
pCMV5F
pCMV-F
M13F/T7
T3
M13F/T7
T7
SP6
M13F
M13F
T7
T7
pDsRED-ex-C1-F
pDsRED-ex-C1-F
pEGFP-N-5’
pEGFP-C-5’
pEGFP-N-5’
pEF-F
pEGFP-C-5’
pEGFP-N-5’
M13F
T7
T7
Primer(R)
T3
pACT2-R
pAS2-1.R
pB42ADR
BAC2
T3
T3/M13R
PBV220R
P2
M13F(-47)
S6
此载体无反向引物
SP6/BGH
BGH
BGH
BGH
SP6
M13R
EBV-R
M13R
此载体无反向引物
T3
T3
T7
pCMV5R
pCMV5R
pCMV-R
SP6/M13R
T7
M13R
BGH
T7
M13R
M13R
SP6
SP6
pEGFP-N-3’(距离很近,一般不用)
pEGFP-N-3’
PDSRED-N-R
pECFP-C-3'
pEGFP-N-3’
pCDNA3.1R
pEGFP-C-3'
pEGFP-N-3’
M13R
T7 Ter
T7 Ter
pET28,30,24,49(KA
N+)
pET32(a,b,c)(AMP+
)
pET50(amp+)
pET44(AMP+)
pEYFP-N1
pEYFP-C1
pFLAG-CMV
pGAD424
pGADGH
pGADT7-Rec
pGAPZa-A
pGBKT7(Kana+)
pGEM-T(-Easy)
pGEX-(*)T
pGL2
pGL3
PinpointTM Vector
pIRES
pIRES2-EGFP
pLXSN
pMAL-c2E
pMAL-C2x
pMAL-p2X
PMD18-T
PMD19-T
pPIC9K(AMP+、
ZEO+）
pPICZa
pPROEXHTA
pQE30or40
pRSET
pSilencer3.1-H1
hygro
pSILENCEV2.0-U6
pSK01-T
pSK-CMV(KAN+)
pSP72
pSport1
pSUPER
pTA2
pT-Adv
pTARGET TM
pTO-T7
pTRC99a-c
pTriPLEx2
pTWIN-1
pUC18(19)/118(119
)
pUCm-T
pVAX1
pWSK29
pXT7
T7
T7/
T7/
T7/
pEGFP-N-5’
pEGFP-C-5’
pFLAG-CMV -F
GAL4 AD
GAL4 AD
GAL4 AD/T7
a-FACTOR
T7
M13F/T7
pGEX-4T-5’
GLP1
RVP3
Pinpoint Primer
PIRES-F/T7
pIRES2-EGFP.P5’
pLXSN-F
MalE primer
P5’
MalE primer
M13R(-48)
M13F(-47)
5’AOX/a-factor
5'AOX/PPICZa-F
M13R(-48)
pQE30-F
T7
M13F(-47)
T7
M13F
T7
T7
SP6/M13F(-47)
T7
M13F/T7(优先使用M13F）
M13F /T7
pTarget.F(在T7前面）/T7
T7
pTRC99C-F
5'pTriPLEx2
T7
M13F(-47)
M13F/T7
T7
T7/M13F/M13F(-47)
T7
T7 Ter
T7 Ter
T7TER
T7 Ter
pEGFP-N-3’
pEGFP-C-3'
pFLAG-CMV -R
3'AD
3'AD
3'AD
3'AOX
3'BD
SP6/M13R
pGEX-4T-3’
GLP2
GLP2(RVP4)(没有特别说明用GLP2)
SP6
T3/PIRES-R (J40720)
pIRES2-EGFP.P3’
pLXSN-R
M13F(-47)
P3’
M13F(-47)
M13F(-47)
M13R(-48)
3’AOX
3'AOX
无反向引物
pQE-R:
T7TER
3.0REV
2.0REV
M13R
T3
SP6
T7/M13R
T3
T3/M13R
M13R
pTarget.R
T7TER
pTRC99C-R
T7
T7TER(客户确认再用）
M13R(-48)
M13R
BGH
T3/M13R/M13R(-48)
SP6
pYES2
pLEXA
pLNCX
pRECEIVER
pSHUTTLE-CMV
pSP64/65
pSTBlue-1
pT7Blue(R)
T7
pLEXA-F
pLNCX-F
CMV-F
PSHUTTLE-CMV-F
SP6
T7
T7
pYes2.R
pLEXA-R
pLNCX-R
此载体无反向引物
PSHUTTLE-CMV-R
此载体无反向引物
SP6
M13F(-47)
Invitrogen中国测序通用引物序列
引物名称
M13R
M13F
M13F(-47)
M13R(-48)
M13(-96)
SP6
T7
T7 terminator
T3
pGEX-4T-5'
pGEX-4T-3'
GLp1
GLp2
RVp3
RVp4
pcDNA3.1R
PinPoint primer
pCMV-F
pCMV-R
pTRC99C-F:
pTRC99C-R:
pCEP-F:
EBV-R :
pIRES2-EGFP.P5’:
pIRES2-EGFP.P3’:
3'AD:
CMV -F
S1
S6
5`AOX1
3`AOX1
α-Factor
GAL4 AD
pACT2-R
pB42ADF:
pB42ADR:
pEGFP-N-5’:
pEGFP-N-3’:
pEGFP-C-5’:
pEGFP-C-3' :
PBV220F:
PBV220R:
U6
2.0rev primer:
3.0rev:
5'TriplEx2
序列(5'-3')
CAG GAA ACA GCT ATG ACC
TGT AAA ACG ACG GCC AGT
CGC CAG GGT TTT CCC AGT CAC GAC
AGC GGA TAA CAA TTT CAC ACA GGA
CCC TCA TAG TTA GCG TAA CG
ATT TAG GTG ACA CTA TAG
TAA TAC GAC TCA CTA TAG GG
TGC TAG TTA TTG CTC AGC GG
ATT AAC CCT CAC TAA AGG GA
GGG CTG GCA AGC CAC GTT TGG TG
CCG GGA GCT GCA TGT GTC AGA GG
TGT ATC TTA TGG TAC TGT AAC TG
CTT TAT GTT TTT GGC GTC TTC CA
CTA GCA AAA TAG GCT GTC CC
GAC GAT AGT CAT GCC CCG CG
TAG AAG GCA CAG TCG AGG
CGT GAC GCG GTG CAG GGC G
TCT AAA AGC TGC GGA ATT GT
TCC AAA CTC ATC AAT GTA TC
TTG CGC CGA CAT CAT AAC
CTG CGT TCT GAT TTA ATC TG
AGA GCT CGT TTA GTG AAC CG
GTG GTT TGT CCA AAC TCA TC
GTA GGC GTG TAC GGT GGG AG
AAC GCA CAC CGG CCT TAT TC
AGA TGG TGC ACG ATG CAC AG
CGC AAA TGG GCG GTA GGC GTG
CAA CGT GAA AAA ATT ATT ATT CGC
GTA AAT GAA TTT TCT GTA GTA GG
GAC TGG TTC CAA TTG ACA AGC
GCA AAT GGC ATT CTG ACA TCC
TAC TAT TGC CAG CAT TGC TGC
TAC CAC TAC AAT GGA TG
GTG CAC GAT GCA CAG TTG AA
CCA GCC TCT TGC TGA GTG GAG ATG
AAG CCG ACA ACC TTG ATT GGA G
TGG GAG GTC TAT ATA AGC AGA G
CGT CGC CGT CCA GCT CGA CCA G
CAT GGT CCT GCT GGA GTT CGT G
TAT GGC TGA TTA TGA TCA GT
AAG AAG GGC AGC ATT CAA AG
CTG CGT TCT GAT TTA ATC TG
ATG GAC TAT CAT ATG CTT ACC GTA
AGG CGA TTA AGT TGG GTA
GAG TTA GCT CAC TCA TTA GGC
GAA CGC CAG CAC ATG GAC
CTC CGA GAT CTG GAC GAG C
RVP4
RVP3
pQE30-R
pQE30-F
pEF-F
pDSRED-N1-R
pDsRED-express-C1-
pCMV5R
pCMV5F
P5'
P3'
BAC2
BAC1
3'BD:
pTarget.F
pTarget.R
T7(17base)
pYes2.R
GAL4 BD
pAS2-1.R
pFlag-CMV-F:
pFlag-CMV-R:
pIRES-F:
pIRES.R:
P1
P2
pLEXA-F
pLEXA-R
pLNCX-F
pLNCX-R
pLXSN-F
pLXSN-R
pSHUTTLE-CMV-F
pSHUTTLE-CMV-R
MalE Primer
GAC GAT AGT CAT GCC CCG CG
CTA GCA AAA TAG GCT GTC CC
GTT CTG AGG TCA TTA CTG G
TGA GCG GAT AAC AAT TTC AC
TCA AGC CTC AGA CAG TGG TTC
TGA AGC GCA TGA ACT CCT TG
TCC CAC AAC GAG GAC TAC AC
ATT ATA GAG GAC ACC TAG TC
TTC CAA AAT GTC GTA ATA AC
TGC GTA CTG CGG TGA TCA AC
CTG CAA GGC GAT TAA GTT GG
ACG CAC AGA ATC TAG CGC TT
AAC CAT CTC GCA AAT AAA TA
TAA GAG TCA CTT TAA AAT TTG TAT AC
CCA GGA TTT TCC CAG TCA C
GGC TTT ACA CTT TAT GCT TC
ACA TCC ACT TTG CCT TTC TC
TCG GTT AGA GCG GAT GTG
TCA TCG GAA GAG AGT AG
AAG AGT TAC TCA AGA ACA AGA A
GGT AGG CGT GTA CGG TGG
GCA CTG GAG TGG CAA CTT
CTT ACT GAC ATC CAC TTT GC
CAC TGC ATT CTA GTT GTG GT
CCA GGC TTT ACA CTT TAT GC
GCG ATT AAG TTG GGT AAC GC
CGT CAG CAG AGC TTC ACC AT
TAA AAC CTA AGA GTC ACT TT
AGC TCG TTT AGT GAA CCG TCA GAT CG
ACC TAC AGG TGG GGT CTT TCA TTC CC
CTT GAA CCT CCT CGT TCG AC
GTT GCT GAC TAA TTG AGA TG
GGT CTA TAT AAG CAG AGG TG
GTG GTA TGG CTG ATT ATG ATC AG
GGT CGT CAG ACT GTC GAT GAA GCC