2024年10月5日发(作者：骆寻绿)
Product datasheet
Rat IgG ELISA Kit, Fluorescent ab229388
3 Images
Overview
Product name
Detection method
Precision
Sample
Rat serum
Rat IgG ELISA Kit, Fluorescent
Fluorescent
Intra-assay
n
3
MeanSDCV%
2.66%
Inter-assay
Sample
Rat serum
n
3
MeanSDCV%
3.61%
Sample type
Assay type
Sensitivity
Range
Recovery
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Sandwich (quantitative)
35 pg/ml
0.04 ng/ml - 160 ng/ml
Sample specific recovery
Sample type
Antibody Diluent 4B
10F DMEM Medium
OF DMEM Medium
Average %
103.7
79.1
76.5
Range
99.4% - 110.97%
67.5% - 98%
62.8% - 94%
Assay time
Assay duration
Species reactivity
Product overview
1h 30m
One step assay
Reacts with:
Rat
Does not react with:
Goat, Cow, Pig
Immunoglobulin G (IgG)
in vitro
CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent
1
Assay) kit is designed for the quantitative measurement of Immunoglobulin G (IgG) protein in rat
serum, plasma, and cell culture supernatant.
This CatchPoint SimpleStep ELISA kit has been
optimized for Molecular Devices Microplate
Readers
. Click
here
for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured
protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and
analysis settings at
.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a
reporter conjugated detector antibody which immunocapture the sample analyte in solution. This
entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via
immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or
standards are added to the wells, followed by the antibody mix. After incubation, the wells are
washed to remove unbound material. CatchPoint HRP Development Solution containing the
Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP
generating a fluorescent product. Signal is generated proportionally to the amount of bound
analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm
Excitation/Cutoff/Emission.
Notes
There are four classes of immunoglobulins in rat: IgA, IgE, IgM, and IgG. IgG is the most abundant
immunoglobulin and is equally distributed in blood and tissue. In rat, the IgG class is further
divided into four subclasses: IgG1, IgG2a, IgG2b, and IgG2c. The general immunoglobulin
structure is composed of four polypeptide chains, two heavy and two light chains linked together
and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting
tetramer creates two identical halves which together form a Y like structure. While the amino-
terminal portions that exhibits highly variable amino-acid composition are involved in antigen
binding, the C terminal constant parts are involved in complement binding, placental passage and
binding to cell membrane.
IgG is involved in response to a foreign antigen and the presence of IgG usually signifies a mature
antibody response. IgG has a molecular weight of about 150 kDa, can bind to many pathogens
and also plays an important role in antibody dependent cell-mediated cytotoxicity. Typically rat
serum and plasma samples contain about 7 to 10 mg/ml of IgG.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Components
100X Stoplight Red Substrate
10X Rat IgG Capture Antibody
10X Rat IgG Detector Antibody
10X Wash Buffer PT (ab206977)
500X Hydrogen Peroxide (H2O2, 3%)
Store at +4°C. Please refer to protocols.
1 x 96 tests
1 x 120µl
1 x 600µl
1 x 600µl
1 x 20ml
1 x 50µl
2
Components
Antibody Diluent 4B
Plate Seals
Rat IgG Lyophilized Purified Protein
Sample Diluent NS (ab193972)
SimpleStep Pre-Coated Black 96-Well Microplate
Stoplight Red Substrate Buffer
1 x 96 tests
1 x 6ml
1 unit
2 vials
1 x 50ml
1 unit
1 x 12ml
Secreted
Cellular localization
Images
SimpleStep ELISA technology allows the formation of the antibody-
antigen complex in one single step, reducing assay time to 90
minutes. Add samples or standards and antibody mix to wells all at
once, incubate, wash, and add your final substrate. See protocol for
a detailed step-by-step guide.
Other - Rat IgG ELISA Kit, Fluorescent (ab229388)
The Immunoglobulin G (IgG) standard curve was prepared as
described in Section 10. Background-subtracted data values (mean
+/- SD) are graphed.
Example of rat Immunoglobulin G (IgG) standard
curve in Sample Diluent NS.
3
Interpolated rat IgG value are graphed.
Example of rat serum IgG level in Sample Diluent
NS.
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit
/abpromise
or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
4
2024年10月5日发(作者：骆寻绿)
Product datasheet
Rat IgG ELISA Kit, Fluorescent ab229388
3 Images
Overview
Product name
Detection method
Precision
Sample
Rat serum
Rat IgG ELISA Kit, Fluorescent
Fluorescent
Intra-assay
n
3
MeanSDCV%
2.66%
Inter-assay
Sample
Rat serum
n
3
MeanSDCV%
3.61%
Sample type
Assay type
Sensitivity
Range
Recovery
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Sandwich (quantitative)
35 pg/ml
0.04 ng/ml - 160 ng/ml
Sample specific recovery
Sample type
Antibody Diluent 4B
10F DMEM Medium
OF DMEM Medium
Average %
103.7
79.1
76.5
Range
99.4% - 110.97%
67.5% - 98%
62.8% - 94%
Assay time
Assay duration
Species reactivity
Product overview
1h 30m
One step assay
Reacts with:
Rat
Does not react with:
Goat, Cow, Pig
Immunoglobulin G (IgG)
in vitro
CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent
1
Assay) kit is designed for the quantitative measurement of Immunoglobulin G (IgG) protein in rat
serum, plasma, and cell culture supernatant.
This CatchPoint SimpleStep ELISA kit has been
optimized for Molecular Devices Microplate
Readers
. Click
here
for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured
protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and
analysis settings at
.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a
reporter conjugated detector antibody which immunocapture the sample analyte in solution. This
entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via
immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or
standards are added to the wells, followed by the antibody mix. After incubation, the wells are
washed to remove unbound material. CatchPoint HRP Development Solution containing the
Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP
generating a fluorescent product. Signal is generated proportionally to the amount of bound
analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm
Excitation/Cutoff/Emission.
Notes
There are four classes of immunoglobulins in rat: IgA, IgE, IgM, and IgG. IgG is the most abundant
immunoglobulin and is equally distributed in blood and tissue. In rat, the IgG class is further
divided into four subclasses: IgG1, IgG2a, IgG2b, and IgG2c. The general immunoglobulin
structure is composed of four polypeptide chains, two heavy and two light chains linked together
and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting
tetramer creates two identical halves which together form a Y like structure. While the amino-
terminal portions that exhibits highly variable amino-acid composition are involved in antigen
binding, the C terminal constant parts are involved in complement binding, placental passage and
binding to cell membrane.
IgG is involved in response to a foreign antigen and the presence of IgG usually signifies a mature
antibody response. IgG has a molecular weight of about 150 kDa, can bind to many pathogens
and also plays an important role in antibody dependent cell-mediated cytotoxicity. Typically rat
serum and plasma samples contain about 7 to 10 mg/ml of IgG.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Components
100X Stoplight Red Substrate
10X Rat IgG Capture Antibody
10X Rat IgG Detector Antibody
10X Wash Buffer PT (ab206977)
500X Hydrogen Peroxide (H2O2, 3%)
Store at +4°C. Please refer to protocols.
1 x 96 tests
1 x 120µl
1 x 600µl
1 x 600µl
1 x 20ml
1 x 50µl
2
Components
Antibody Diluent 4B
Plate Seals
Rat IgG Lyophilized Purified Protein
Sample Diluent NS (ab193972)
SimpleStep Pre-Coated Black 96-Well Microplate
Stoplight Red Substrate Buffer
1 x 96 tests
1 x 6ml
1 unit
2 vials
1 x 50ml
1 unit
1 x 12ml
Secreted
Cellular localization
Images
SimpleStep ELISA technology allows the formation of the antibody-
antigen complex in one single step, reducing assay time to 90
minutes. Add samples or standards and antibody mix to wells all at
once, incubate, wash, and add your final substrate. See protocol for
a detailed step-by-step guide.
Other - Rat IgG ELISA Kit, Fluorescent (ab229388)
The Immunoglobulin G (IgG) standard curve was prepared as
described in Section 10. Background-subtracted data values (mean
+/- SD) are graphed.
Example of rat Immunoglobulin G (IgG) standard
curve in Sample Diluent NS.
3
Interpolated rat IgG value are graphed.
Example of rat serum IgG level in Sample Diluent
NS.
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit
/abpromise
or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
4