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对GBT4789.3—2008标准的理解与应用

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2024年3月16日发(作者:忻宛畅)

研究与探讨 中国标准导报 

CHINA STANDARDS REVlEW 

对GB/T 4789.3—2008标准的理解与应用 

裴鸣宇 陈晶 

国家新颁布的GB/T 4789.3—2OO8《食品卫生 

发酵管中利用胆盐作为抑菌剂,新标准中则以月桂 

微生物检验大肠菌群计数》标准,在2009年3月 

基硫酸盐胰蛋白胨肉汤中利用十二烷基硫酸钠(月 

1日已正式实施了。 

桂基)作为抑菌剂。两者比较,月桂基比胆盐更能 

本人在学习与应用中理解并体会到,新标准与 

有效地抑制革兰氏阳性菌的生长,同时比胆盐的选 

GB/T 4789.3—2OO3相比主要不同点和在实施过 

择性和稳定性好,抑制剂可抑制样品中目标菌株外 

程中应注意的几点问题: 

的一些杂菌,而有利于大肠菌群细菌的生长和挑 

1 新标准将原标准拆分并改名称 选,但对大肠菌群中的某些菌株有时也会产生一些 

新标准将原标准拆分为大肠菌群计数、粪大肠 抑制作用。有时抑菌剂在培养基中用量甚微,称量 

菌群计数和大肠杆菌计数三个独立的标准方法。 

时略有误差,即可对目标菌产生抑制作用,从而影 

标准名称有所变化,将大肠菌群测定改为大肠菌群 

响检测结果,因此抑菌剂的添加应严格按照标准方 

计数。 

法进行。 

在新标准中除了MPN法还增加了大肠菌群平 4新标准将原标准中“报告每100 mL(g)大肠菌 

板计数和纸片法,这些方法常常一步就能检测出大 群的MPN值”修改为“报告每1 mL(g)大肠菌群的 

肠菌群的存在,而且使用的时问较短,但对于大肠 

MPN值” 

菌群含量低的样品这个方法不适用。杂菌污染高, 目前我国许多产品的卫生标准中大肠菌群还 

大肠菌群含量低的样品应使用MPN法。 

是以MPN/100 mL(g)表示,所以在执行新标准以 

2新标准对样液的pH值有了范围规定 

后得出的检验结果MPN/mI (g)必须换算成 

各种食品都有一定酸碱范围,只有接近中性的 MPN/100 mI (g)才能与产品卫生标准相一致。 

食品大多数微生物生长良好,过高或过低的pH值 

例如:我们依据GB/T 4789.3~2008标准对面 

对某些微生物生长繁殖不利,受到一定程度的限 

包进行大肠菌群计数检验。首先,用无菌镊子夹 

制。另一方面,微生物在食品内生长繁殖过程中也 去外包装,取外部及中心部分共25 g面包,放入 

可使其pH值发生改变,从而导致微生物群类(菌 

盛有225 mI 灭菌生理盐水的无菌均质器内, 

相)的相应变化,在新标准中规定样液的pH值范 

8 000 r/min ̄10 000 r/min均质1 rain~2 min之 

围在6.5~7.5之间,必要时分别用1 mol/I NaOH 

问,必要时分别用1 mol/I NaOH或1 mol/I HC1 

或1 mol/I HC1以调节,在调节过程中不能使用浓 

进行调节,制成1:10样品匀液,用1 mI 无菌吸管 

度过高的酸或碱,严格遵守标准中要求的浓度,否 吸取1:10样液1 mI 沿吸管壁缓慢放入盛有 

则浓度过高产生热量,细菌易损伤,造成检验结果 

9 mI 灭菌生理盐水中,振摇试管制成1:100的样 

的误差加大。 

品匀液。 

3新标准中MPN法培养基的改变 

按以上操作程序制备1:1 000样品匀液。我 

新标准中的MPN法是以旧标准中乳糖胆盐为 们按标准中要求选择三个稀释度的样液1:1O、 

主要培养基改为以月桂基硫酸盐胰蛋白胨肉汤为 

1:100、1:1 000,各接种3管月桂基硫酸盐胰蛋白 

主要培养基。GB/T 4789.3 2003中以乳糖胆盐 胨肉汤(LST),每个稀释度 (下转第39页) 

24 

标准新干线 中国标准导报 

CHINA STANDARDS REVIEW 

171 

1 72 

GI3/T 24800.8 2OO9 

GB/T 24800.9 2OO9 

化妆品中甲氨嘌呤的测定 高效液相色谱法 

化妆品中柠檬醛、肉桂醇、茴香醇、肉桂醛和香 

素的测定气相色谱法 

化妆品中十九种香料的测定 气相色谱质潜法 

2O】O O5 O1 

2O10 05 0】 

173 GB/T 24800 

1O 2OO9 

2O1O O5 O1 

174 

1 75 

l76 

Gt3/T 24800 

11—20O9 

2一一2OO9 

G]3/T 24800 

l 

化妆品中防腐剂苯甲醇的测定气相色谱法 

2O10—05—01 

2O10 05—01 

2O10—05—01 

2010 05—01 

2O10 05 01 

化妆品中对苯二胺、邻苯二胺和间苯二胺的测定 

化妆品中、 硝酸盐的测定 离子色谱法 

1000kV变电站监控系统技术规范 

1000kV交流架空输电线路金具技术规范 

GB/ T 24800 

】3—20O9 

177 

l78 

G]3/T 24833 

2OO9 

 

GB/T 24834 

2OO9

179 G13/Z 24835 

2OO9 

1000kV气体绝缘金属封闭开关设备运行及维护 

规程 

2OlO—O5 Ol 

1 8O GB/Z 24836—2OO9 

l100kV气体绝缘金属封闭开关设备技术规范 

2()1O O5 0l 

2O1O O5一O1 

181 GB/Z 24837 2OO9 

1 100kV高压交流隔离开关和接地开关技术规范 

1100kV高压交流断路器技术规范 

1000kV交流系统用支柱绝缘子技术规范 

1000kV交流系统用套管技术规范 

1000kV交流系统用电容式电压互感器技术规范 

182 

183 

G13/Z 24838 2OO9 

GB/Z 24839—2OO9 

2O1O—O5—01 

2O1 O~O5一O1 

2Ol0 O5—01 

201O—O5一O1 

2O1O O5 01 

l84 

185 

186 

G]3/z 24840 2009 

GI3/Z 2484l一2OO9 

GB/Z 24842 2OO9 

1000kV特高压交流输变电工程过电压和绝缘 

配合 

1000kV单相油浸式自耦电力变压器技术规范 

1000kV交流系统用油浸式并联电抗器技术规范 

1000kV交流系统用无问隙金属氧化物避雷器技 

术规范 

l87 G13/Z 24843--2009 

201O O5 0l 

2O1O一05一O1 

2O10 05 01 

188 

189 

GB/Z 24844—2009 

GB/Z 24845 2009 

l90 

】91 

G13/Z 24846—2009 

GB/Z 24847 2009 

l000kV交流电气设备预防性试验规程 

1000kV交流系统电压和无功电力技术导则 

2OlO 05 Ol 

2O1O一05 O1 

,,,m,,,,,.1ll,Ip-,,,’,’''''lib'’',,',,,',','l ’,,'',,l’’l,,',',,'’’m,m,,,,',,’,,''''l',●,’,,’',' 

(上接第24页) 

换用一次1 mI,无菌吸管,每管接种1 mI ,36℃± 

1 oC培养48 h±2 h,观察管内是否有气泡产生,未 

产气者为大肠菌群阴性,产气者则进行复发酵试 

验,用接种环从所有发酵产气的LST肉汤之中分别 

取培养物2环,接种于煌绿乳糖胆盐肉汤管中,36℃ 

量,并非稀释后的量,对固体样品更应注意,如固体 

样品经1O倍稀释后,虽加入1 mI 的量,但实际其中 

只有0.1 g样品,所以按以上操作进行检验时,采用3 

个稀释度1:1O、1:100、1:1 000,在LST肉汤管中, 

必须分别加入3管10 mI 样液(1:10),1 mI 样液 

(1:100),1 mL样液(1:1 000),加入实际样品量 

分别是1 g,0.1 g,0.01 g,查MPN检索表, 

<O.3 MPN/g(标准中规定增大几倍检样量,结果 

降低几倍),换算<O.3 MPN/g ̄H当于<30 MPN/ 

100 g,这样就与面包糕点的卫生标准中规定的大肠 

±1℃培养48 h±2 h,观察产气情况,产气者即为大 

肠菌群阳性,根据大肠菌群阳性管数,查MPN检索 

表,报告每克样品中大肠菌群的MPN值;如果以上试 

验LST肉汤管都没有产气,查MPN表结果是大肠菌 

群计数<3.0 MPN/g,换算<3.0 MPN/g相当于 

<300 MPN/100 g,但GB 7099--2003《糕点、面包 

菌群计数相一致了。 

但值得注意的是在加大检验量的同时,接种量 

卫生标准》中大肠菌群要求是<30 MPN/i00 g,所 

以<3 O(j MPN/100 g与<3O MPN/100 g两个数据 

无法进行比较,不能判定是否合格。为了使检验 

也相对增大了,例如:1:10的稀释度样液,如果检 

样量增大到1 g(mL)接种量是l0 mL,新标准中规 

结果与产品卫生标准换算后的MPN值相符,我 

们在检验时不能采用MPN检查表给出的3个稀 

释度即0.1 mL(g)、0.01 mI (g)、0.001 mL(g)应 

定接种量如果超过1 mL,I ST肉汤用双料管,否则 

的话,增大了I ST肉汤培养基稀释度,造成营养成 

分不足,导致结果有误差。 

该增大检验量,在MPN检索表第一栏阳性管数下 

(作者单位:吉林省长春市产品质量监督检验院) 

面列出的mL(g)是指原样品(包括液体和固体)的 

39 

2024年3月16日发(作者:忻宛畅)

研究与探讨 中国标准导报 

CHINA STANDARDS REVlEW 

对GB/T 4789.3—2008标准的理解与应用 

裴鸣宇 陈晶 

国家新颁布的GB/T 4789.3—2OO8《食品卫生 

发酵管中利用胆盐作为抑菌剂,新标准中则以月桂 

微生物检验大肠菌群计数》标准,在2009年3月 

基硫酸盐胰蛋白胨肉汤中利用十二烷基硫酸钠(月 

1日已正式实施了。 

桂基)作为抑菌剂。两者比较,月桂基比胆盐更能 

本人在学习与应用中理解并体会到,新标准与 

有效地抑制革兰氏阳性菌的生长,同时比胆盐的选 

GB/T 4789.3—2OO3相比主要不同点和在实施过 

择性和稳定性好,抑制剂可抑制样品中目标菌株外 

程中应注意的几点问题: 

的一些杂菌,而有利于大肠菌群细菌的生长和挑 

1 新标准将原标准拆分并改名称 选,但对大肠菌群中的某些菌株有时也会产生一些 

新标准将原标准拆分为大肠菌群计数、粪大肠 抑制作用。有时抑菌剂在培养基中用量甚微,称量 

菌群计数和大肠杆菌计数三个独立的标准方法。 

时略有误差,即可对目标菌产生抑制作用,从而影 

标准名称有所变化,将大肠菌群测定改为大肠菌群 

响检测结果,因此抑菌剂的添加应严格按照标准方 

计数。 

法进行。 

在新标准中除了MPN法还增加了大肠菌群平 4新标准将原标准中“报告每100 mL(g)大肠菌 

板计数和纸片法,这些方法常常一步就能检测出大 群的MPN值”修改为“报告每1 mL(g)大肠菌群的 

肠菌群的存在,而且使用的时问较短,但对于大肠 

MPN值” 

菌群含量低的样品这个方法不适用。杂菌污染高, 目前我国许多产品的卫生标准中大肠菌群还 

大肠菌群含量低的样品应使用MPN法。 

是以MPN/100 mL(g)表示,所以在执行新标准以 

2新标准对样液的pH值有了范围规定 

后得出的检验结果MPN/mI (g)必须换算成 

各种食品都有一定酸碱范围,只有接近中性的 MPN/100 mI (g)才能与产品卫生标准相一致。 

食品大多数微生物生长良好,过高或过低的pH值 

例如:我们依据GB/T 4789.3~2008标准对面 

对某些微生物生长繁殖不利,受到一定程度的限 

包进行大肠菌群计数检验。首先,用无菌镊子夹 

制。另一方面,微生物在食品内生长繁殖过程中也 去外包装,取外部及中心部分共25 g面包,放入 

可使其pH值发生改变,从而导致微生物群类(菌 

盛有225 mI 灭菌生理盐水的无菌均质器内, 

相)的相应变化,在新标准中规定样液的pH值范 

8 000 r/min ̄10 000 r/min均质1 rain~2 min之 

围在6.5~7.5之间,必要时分别用1 mol/I NaOH 

问,必要时分别用1 mol/I NaOH或1 mol/I HC1 

或1 mol/I HC1以调节,在调节过程中不能使用浓 

进行调节,制成1:10样品匀液,用1 mI 无菌吸管 

度过高的酸或碱,严格遵守标准中要求的浓度,否 吸取1:10样液1 mI 沿吸管壁缓慢放入盛有 

则浓度过高产生热量,细菌易损伤,造成检验结果 

9 mI 灭菌生理盐水中,振摇试管制成1:100的样 

的误差加大。 

品匀液。 

3新标准中MPN法培养基的改变 

按以上操作程序制备1:1 000样品匀液。我 

新标准中的MPN法是以旧标准中乳糖胆盐为 们按标准中要求选择三个稀释度的样液1:1O、 

主要培养基改为以月桂基硫酸盐胰蛋白胨肉汤为 

1:100、1:1 000,各接种3管月桂基硫酸盐胰蛋白 

主要培养基。GB/T 4789.3 2003中以乳糖胆盐 胨肉汤(LST),每个稀释度 (下转第39页) 

24 

标准新干线 中国标准导报 

CHINA STANDARDS REVIEW 

171 

1 72 

GI3/T 24800.8 2OO9 

GB/T 24800.9 2OO9 

化妆品中甲氨嘌呤的测定 高效液相色谱法 

化妆品中柠檬醛、肉桂醇、茴香醇、肉桂醛和香 

素的测定气相色谱法 

化妆品中十九种香料的测定 气相色谱质潜法 

2O】O O5 O1 

2O10 05 0】 

173 GB/T 24800 

1O 2OO9 

2O1O O5 O1 

174 

1 75 

l76 

Gt3/T 24800 

11—20O9 

2一一2OO9 

G]3/T 24800 

l 

化妆品中防腐剂苯甲醇的测定气相色谱法 

2O10—05—01 

2O10 05—01 

2O10—05—01 

2010 05—01 

2O10 05 01 

化妆品中对苯二胺、邻苯二胺和间苯二胺的测定 

化妆品中、 硝酸盐的测定 离子色谱法 

1000kV变电站监控系统技术规范 

1000kV交流架空输电线路金具技术规范 

GB/ T 24800 

】3—20O9 

177 

l78 

G]3/T 24833 

2OO9 

 

GB/T 24834 

2OO9

179 G13/Z 24835 

2OO9 

1000kV气体绝缘金属封闭开关设备运行及维护 

规程 

2OlO—O5 Ol 

1 8O GB/Z 24836—2OO9 

l100kV气体绝缘金属封闭开关设备技术规范 

2()1O O5 0l 

2O1O O5一O1 

181 GB/Z 24837 2OO9 

1 100kV高压交流隔离开关和接地开关技术规范 

1100kV高压交流断路器技术规范 

1000kV交流系统用支柱绝缘子技术规范 

1000kV交流系统用套管技术规范 

1000kV交流系统用电容式电压互感器技术规范 

182 

183 

G13/Z 24838 2OO9 

GB/Z 24839—2OO9 

2O1O—O5—01 

2O1 O~O5一O1 

2Ol0 O5—01 

201O—O5一O1 

2O1O O5 01 

l84 

185 

186 

G]3/z 24840 2009 

GI3/Z 2484l一2OO9 

GB/Z 24842 2OO9 

1000kV特高压交流输变电工程过电压和绝缘 

配合 

1000kV单相油浸式自耦电力变压器技术规范 

1000kV交流系统用油浸式并联电抗器技术规范 

1000kV交流系统用无问隙金属氧化物避雷器技 

术规范 

l87 G13/Z 24843--2009 

201O O5 0l 

2O1O一05一O1 

2O10 05 01 

188 

189 

GB/Z 24844—2009 

GB/Z 24845 2009 

l90 

】91 

G13/Z 24846—2009 

GB/Z 24847 2009 

l000kV交流电气设备预防性试验规程 

1000kV交流系统电压和无功电力技术导则 

2OlO 05 Ol 

2O1O一05 O1 

,,,m,,,,,.1ll,Ip-,,,’,’''''lib'’',,',,,',','l ’,,'',,l’’l,,',',,'’’m,m,,,,',,’,,''''l',●,’,,’',' 

(上接第24页) 

换用一次1 mI,无菌吸管,每管接种1 mI ,36℃± 

1 oC培养48 h±2 h,观察管内是否有气泡产生,未 

产气者为大肠菌群阴性,产气者则进行复发酵试 

验,用接种环从所有发酵产气的LST肉汤之中分别 

取培养物2环,接种于煌绿乳糖胆盐肉汤管中,36℃ 

量,并非稀释后的量,对固体样品更应注意,如固体 

样品经1O倍稀释后,虽加入1 mI 的量,但实际其中 

只有0.1 g样品,所以按以上操作进行检验时,采用3 

个稀释度1:1O、1:100、1:1 000,在LST肉汤管中, 

必须分别加入3管10 mI 样液(1:10),1 mI 样液 

(1:100),1 mL样液(1:1 000),加入实际样品量 

分别是1 g,0.1 g,0.01 g,查MPN检索表, 

<O.3 MPN/g(标准中规定增大几倍检样量,结果 

降低几倍),换算<O.3 MPN/g ̄H当于<30 MPN/ 

100 g,这样就与面包糕点的卫生标准中规定的大肠 

±1℃培养48 h±2 h,观察产气情况,产气者即为大 

肠菌群阳性,根据大肠菌群阳性管数,查MPN检索 

表,报告每克样品中大肠菌群的MPN值;如果以上试 

验LST肉汤管都没有产气,查MPN表结果是大肠菌 

群计数<3.0 MPN/g,换算<3.0 MPN/g相当于 

<300 MPN/100 g,但GB 7099--2003《糕点、面包 

菌群计数相一致了。 

但值得注意的是在加大检验量的同时,接种量 

卫生标准》中大肠菌群要求是<30 MPN/i00 g,所 

以<3 O(j MPN/100 g与<3O MPN/100 g两个数据 

无法进行比较,不能判定是否合格。为了使检验 

也相对增大了,例如:1:10的稀释度样液,如果检 

样量增大到1 g(mL)接种量是l0 mL,新标准中规 

结果与产品卫生标准换算后的MPN值相符,我 

们在检验时不能采用MPN检查表给出的3个稀 

释度即0.1 mL(g)、0.01 mI (g)、0.001 mL(g)应 

定接种量如果超过1 mL,I ST肉汤用双料管,否则 

的话,增大了I ST肉汤培养基稀释度,造成营养成 

分不足,导致结果有误差。 

该增大检验量,在MPN检索表第一栏阳性管数下 

(作者单位:吉林省长春市产品质量监督检验院) 

面列出的mL(g)是指原样品(包括液体和固体)的 

39 

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File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1729732965)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1729732965)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1729780022)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1729780022)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1729835953)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1729835953)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1729924188)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1729924188)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1729945958)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1729945958)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1730221908)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1730221908)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1730236764)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1730236764)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1730374699)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1730374699)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1734832652)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1734832652)
File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
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