2024年4月16日发(作者：卜和璧)
his-sumo 分子量
英文回答：
The molecular weight of his-sumo is approximately 27
kDa (kilodaltons). The his-sumo fusion protein consists of
a polyhistidine tag (His-tag) fused to a small ubiquitin-
like modifier (SUMO). The His-tag is typically 6-10 amino
acids long and allows for the purification of the fusion
protein using immobilized metal affinity chromatography
(IMAC). The SUMO tag is approximately 100 amino acids long
and plays a role in protein stability, localization, and
interactions. The molecular weight of his-sumo may vary
slightly depending on the specific fusion protein construct
and the presence of any additional tags or modifications.
The molecular weight of a protein can be estimated
using various methods, including gel electrophoresis, mass
spectrometry, and theoretical calculations. Gel
electrophoresis involves separating proteins based on their
size and charge, and the molecular weight can be estimated
by comparing the migration of the protein to protein
standards of known molecular weights. Mass spectrometry is
a technique that directly measures the mass-to-charge ratio
of a protein, providing an accurate determination of the
molecular weight. Theoretical calculations can be used to
predict the molecular weight of a protein based on its
amino acid sequence and known molecular weights of
individual amino acids.
中文回答：
his-sumo分子的分子量约为27 kDa（千道尔顿）。his-sumo
融合蛋白由聚组氨酸标签（His标签）融合到小的泛素样修饰剂
（SUMO）组成。His标签通常长度为6-10个氨基酸，允许使用固定
化金属亲和层析（IMAC）纯化融合蛋白。SUMO标签长度约为100个
氨基酸，在蛋白质稳定性、定位和相互作用中起到一定作用。his-
sumo的分子量可能会根据特定的融合蛋白构建以及任何其他标签或
修饰的存在而略有不同。
可以使用多种方法估算蛋白质的分子量，包括凝胶电泳、质谱
和理论计算。凝胶电泳涉及根据蛋白质的大小和电荷分离蛋白质，
并且可以通过将蛋白质的迁移率与已知分子量的蛋白质标准进行比
较来估计分子量。质谱技术直接测量蛋白质的质荷比，从而准确测
定分子量。理论计算可用于基于蛋白质的氨基酸序列和各个氨基酸
的已知分子量来预测蛋白质的分子量。
2024年4月16日发(作者：卜和璧)
his-sumo 分子量
英文回答：
The molecular weight of his-sumo is approximately 27
kDa (kilodaltons). The his-sumo fusion protein consists of
a polyhistidine tag (His-tag) fused to a small ubiquitin-
like modifier (SUMO). The His-tag is typically 6-10 amino
acids long and allows for the purification of the fusion
protein using immobilized metal affinity chromatography
(IMAC). The SUMO tag is approximately 100 amino acids long
and plays a role in protein stability, localization, and
interactions. The molecular weight of his-sumo may vary
slightly depending on the specific fusion protein construct
and the presence of any additional tags or modifications.
The molecular weight of a protein can be estimated
using various methods, including gel electrophoresis, mass
spectrometry, and theoretical calculations. Gel
electrophoresis involves separating proteins based on their
size and charge, and the molecular weight can be estimated
by comparing the migration of the protein to protein
standards of known molecular weights. Mass spectrometry is
a technique that directly measures the mass-to-charge ratio
of a protein, providing an accurate determination of the
molecular weight. Theoretical calculations can be used to
predict the molecular weight of a protein based on its
amino acid sequence and known molecular weights of
individual amino acids.
中文回答：
his-sumo分子的分子量约为27 kDa（千道尔顿）。his-sumo
融合蛋白由聚组氨酸标签（His标签）融合到小的泛素样修饰剂
（SUMO）组成。His标签通常长度为6-10个氨基酸，允许使用固定
化金属亲和层析（IMAC）纯化融合蛋白。SUMO标签长度约为100个
氨基酸，在蛋白质稳定性、定位和相互作用中起到一定作用。his-
sumo的分子量可能会根据特定的融合蛋白构建以及任何其他标签或
修饰的存在而略有不同。
可以使用多种方法估算蛋白质的分子量，包括凝胶电泳、质谱
和理论计算。凝胶电泳涉及根据蛋白质的大小和电荷分离蛋白质，
并且可以通过将蛋白质的迁移率与已知分子量的蛋白质标准进行比
较来估计分子量。质谱技术直接测量蛋白质的质荷比，从而准确测
定分子量。理论计算可用于基于蛋白质的氨基酸序列和各个氨基酸
的已知分子量来预测蛋白质的分子量。