2024年5月22日发(作者：贺坚诚)
常见载体引物表
Vector
PBAD
pACT
pACT2
pAS2-1
pB42AD
pBACPAK8
pBK-CMV
PBS(SK/KS)/M13-
pBV220
pCAMBIA 1301(1300)
pCAMBIA 2300
PCANTB5E
pCAT3-enhancer
pcDNA4
pcDNA6
pcDNAII
pCEP4
pCF-T
pCI
pCI-neo
pCMS-EGFP
pCMV-3Tag-4A
pCMV5
pCMV5-Flag
pCMV-MYC/NUC
PCMV-HA
pCMV-Sport
pCMV-Tag(KAN+)
pCS2
pDonar
pDONR221
pDrive
pDRIveR(KAN+)
pDsRED1-C1
pDsRed1-N1
pDsRed-Monomer-N1
pDsRED2-C1(KAN+)
pDSRED-N1(KAN+)
Primer(F)
PBAD-F/TRXF
T7
Ac5-Profor
GAL4-AD-Cfor
GAL4 BD
pB42ADF
BAC1
T7
M13F/T7
PBV220F
P1
M13R(-48)
S1/M13R
RVP3
CMV-F/T7
CMV-F/T7
T7/CMV-F
Primer(R)
PBAD-R
T3
BGH
pGAL4-ADrv
pB42ADR
BAC2
T3
T3/M13R
PBV220R
P2〔同2.0-rev〕
M13F(-47)
S6
此载体无反向引物
SP6/BGH
BGH
BGH
T7/CMV-F(J21025在T7前面〕
BGH
T7 SP6
M13F M13R
pCEP-F EBV-R
M13F M13R
T7〔17Base〕 此载体无反向引物
T7〔17Base〕 T3
T7 T3
T3 /PFLAG-CMV-F T7
pCMV5F pCMV5R
pCMV5F pCMV5R
CMV-F BGH
CMV-F EBV-R
M13F/T7 SP6/M13R
T3 T7
M13F/T7 M13R
T7 BGH
SP6 T7
M13F M13R
M13F M13R
T7 SP6
T7 SP6
pEGFP-N-3’(距离很近,一般不
pDsRED-ex-C1-F
CMV-Profor
CMV-Profor
用)
RFP-Nrev
DSRED1_n_primer
pDsRED-ex-C1-F
pEGFP-N-5’
pEGFP-N-3’
PDSRED-N-R
pECFP-C
pECFP-N1
pEF/myc/ER
pEGFP-C(KAN+)
pEGFP-N(KAN+)
pENTR/D-TOPO(KAN+)
pET-*(His)(KAN+)
pET22(a,b,c)(AMP+)
pET28,30,24,49(KAN+)
pET32(a,b,c)(AMP+)
pET-42a (-b, -c)(+)
pET50(amp+)
pET44(AMP+)
pEYFP-N1
PETBlue
pEYFP-C1
pFLAG-CMV
pGAD424
pGADGH
pGADT7-Rec
pGAPZa-A
pGBKT7(Kana+)
pGEM-T(-Easy)
pGEX-(*)T
pGL2
pGL3
PinpointTM Vector
pIRES
pIRES2-EGFP
pLXSN
pMAL-c2E
pMAL-C2x
pMAL-p2X
PMD18-T
PMD19-T
pPIC9K(AMP+、ZEO+〕
pPICZa
pPROEXHTA
pQE30or40
pRSET
pSilencer3.1-H1 hygro
pSK01-T
pSK-CMV(KAN+)
pSP72
pSport1
pSUPER
pTA2
pT-Adv
pEGFP-C-5’
pEGFP-N-5’
pEF-F
pEGFP-C-5’
pEGFP-N-5’
M13F
T7
T7
T7
Stag
pEGFP-N-5’
PETBlueT7UP(太靠前，在
T7前)/ PETBlueUP
pEGFP-C-5’
CMV30
GAL4 AD
GAL4 AD
GAL4 AD/T7
a-FACTOR
T7
M13F/T7
pGEX-4T-5’
GLP1
RVP3
Pinpoint Primer
PIRES-F/T7
5’
pLXSN-F
MalE primer
P5’
MalE primer
M13R(-48)
M13F(-47)
5’AOX/a-factor
5'AOX/PPICZa-F
M13R(-48)
pQE30-F
T7
M13F(-47)
T7
M13F
T7
T7
SP6/M13F(-47)
T7
M13F/T7(优先使用M13F〕
M13F /T7
pECFP-C-3'
pEGFP-N-3’
pEGFP-C-3'
pEGFP-N-3’
M13R
T7 Ter
T7 Ter
T7 Ter
T7 Ter
T7 Ter
T7TER
T7 Ter
pEGFP-N-3’
PETBlueDOWN
pEGFP-C-3'
CMV-24
3'AD
3'AD
3'AD
3'AOX
3'BD
SP6/M13R
pGEX-4T-3’
GLP2
GLP2(RVP4)
SP6
T3/PIRES-R (J40720)
3’
pLXSN-R
M13F(-47)
P3’
M13F(-47)
M13F(-47)
M13R(-48)
3’AOX
3'AOX
无反向引物
pQE-R:
T7TER
M13R
T3
SP6
T7/M13R
T3
T3/M13R
M13R
pTARGET TM
pTO-T7
pTRC99a-c
pTriPLEx2
pTWIN-1
pUC18(19)/118(119)
pUC57
pUCm-T
pVAX1
pWSK29
pXT7
pYES2
pLEXA
pLNCX
pRECEIVER
pSHUTTLE-CMV
pSP64/65
pSTBlue-1
pT7Blue(R)
pRK5
pMSCV
pBABE
pFastBac HT_A (_B, _C)
pFastBac1
pACYCDuet-1 (MCS I)
pDSRED2-N1(KAN+)
pFastBac Dual (MCSI)
pBSN-T
pHISi－1
pRSV-Rev(根据酶切位点选
择不同引物)
EZ-T
pMSCV-hyg
PTHIOHIS (A )
PDEST22
pLXSN
pLEXA
pCDH
PVX
pAcGP67 (_A, _B, _C)
pAD-Track
PLPC
pHAGE-CMV-MCS-IZsGreen
PLENTI-HIS
pDSRED-PRESS-C1
pCR-Blunt
pMT/V5-His_A (_B,_C)
pEASY-T3
pTarget.F(在T7前面〕/T7 M13R
T7
pTRC99C-F
5'pTriPLEx2
T7
M13F(-47)
M13R(-48)/M13R
M13F/T7
T7
T7/M13F/M13F(-47)
T7
T7
pLEXA-F
pLNCX-F
CMV-F
PSHUTTLE-CMV-F
SP6
T7
T7
SP6
MSCV5'
pBABE5’
pFastBac-F
PH-Profor/BaculoVfor
T7TER
pTRC99C-R
T7
T7TER(客户确认再用〕
M13R(-48)
M13F(-47)/M13F
M13R
BGH
T3/M13R/M13R(-48)
SP6
pLEXA-R
pLNCX-R
此载体无反向引物
PSHUTTLE-CMV-R
此载体无反向引物
SP6
M13F(-47)
pSG5-3' or SV40pA-R
MSCV-rev
pBABE3’
pFastBac-R
pFastBac-R
DuetDOWN1
DsRed1-N
pFastBac-R/SV40-pArev
T3
无
ACYCDuetUP 1
pEGFP-N-5
BaculoVfor/PH
T7
PHISi-1
pREPF/M13F
HIV-Rev-NES/pGEX-3'
M13F(-47)
MSCV5'
TRXF(new)
PDEST22-F
pLXSNfw
pLEXA-F
pCDH-EF1
LBA
PH
pAD-Track-F
PLPC-F/CMV-F
pHAGE-CMV-F
CMV-F
pDSRED -C1-F
M13R
MT-Profor
T7/M13F
T7
M13R(-48)
PMSCV-R/
MSCV-rev
M13F
PDEST22-R
pLXSNrv
pLEXA-R
无
LBB
pAcGP67rv
无
PLPC-R
暂没设计
无
pDSRED-C1-R
M13F/T7
BGHrev
SP6/M13R
2.0REV(同
P2
(pCAMBIA 1301))
pLVX-IRES-ZsGreen1 PIRES2-EGFP-F PIRES2-EGFP-R
pDC316
PVAX1
PSHUTTLE-CMV
pSilencer™ 4.1-CMV neo
pDC316F
PLXSN-5
T7/CMV-F
pShuttle-CMV-F
pDC316R
PLXSN-3
BGH
pShuttle-CMV-R
P2
(pCAMBIA 1301)
M13F
PSI-CHECK2-F
CMV-F
PSICHECF-2-R1
pMIR-report
PSI-CHECK2
2024年5月22日发(作者：贺坚诚)
常见载体引物表
Vector
PBAD
pACT
pACT2
pAS2-1
pB42AD
pBACPAK8
pBK-CMV
PBS(SK/KS)/M13-
pBV220
pCAMBIA 1301(1300)
pCAMBIA 2300
PCANTB5E
pCAT3-enhancer
pcDNA4
pcDNA6
pcDNAII
pCEP4
pCF-T
pCI
pCI-neo
pCMS-EGFP
pCMV-3Tag-4A
pCMV5
pCMV5-Flag
pCMV-MYC/NUC
PCMV-HA
pCMV-Sport
pCMV-Tag(KAN+)
pCS2
pDonar
pDONR221
pDrive
pDRIveR(KAN+)
pDsRED1-C1
pDsRed1-N1
pDsRed-Monomer-N1
pDsRED2-C1(KAN+)
pDSRED-N1(KAN+)
Primer(F)
PBAD-F/TRXF
T7
Ac5-Profor
GAL4-AD-Cfor
GAL4 BD
pB42ADF
BAC1
T7
M13F/T7
PBV220F
P1
M13R(-48)
S1/M13R
RVP3
CMV-F/T7
CMV-F/T7
T7/CMV-F
Primer(R)
PBAD-R
T3
BGH
pGAL4-ADrv
pB42ADR
BAC2
T3
T3/M13R
PBV220R
P2〔同2.0-rev〕
M13F(-47)
S6
此载体无反向引物
SP6/BGH
BGH
BGH
T7/CMV-F(J21025在T7前面〕
BGH
T7 SP6
M13F M13R
pCEP-F EBV-R
M13F M13R
T7〔17Base〕 此载体无反向引物
T7〔17Base〕 T3
T7 T3
T3 /PFLAG-CMV-F T7
pCMV5F pCMV5R
pCMV5F pCMV5R
CMV-F BGH
CMV-F EBV-R
M13F/T7 SP6/M13R
T3 T7
M13F/T7 M13R
T7 BGH
SP6 T7
M13F M13R
M13F M13R
T7 SP6
T7 SP6
pEGFP-N-3’(距离很近,一般不
pDsRED-ex-C1-F
CMV-Profor
CMV-Profor
用)
RFP-Nrev
DSRED1_n_primer
pDsRED-ex-C1-F
pEGFP-N-5’
pEGFP-N-3’
PDSRED-N-R
pECFP-C
pECFP-N1
pEF/myc/ER
pEGFP-C(KAN+)
pEGFP-N(KAN+)
pENTR/D-TOPO(KAN+)
pET-*(His)(KAN+)
pET22(a,b,c)(AMP+)
pET28,30,24,49(KAN+)
pET32(a,b,c)(AMP+)
pET-42a (-b, -c)(+)
pET50(amp+)
pET44(AMP+)
pEYFP-N1
PETBlue
pEYFP-C1
pFLAG-CMV
pGAD424
pGADGH
pGADT7-Rec
pGAPZa-A
pGBKT7(Kana+)
pGEM-T(-Easy)
pGEX-(*)T
pGL2
pGL3
PinpointTM Vector
pIRES
pIRES2-EGFP
pLXSN
pMAL-c2E
pMAL-C2x
pMAL-p2X
PMD18-T
PMD19-T
pPIC9K(AMP+、ZEO+〕
pPICZa
pPROEXHTA
pQE30or40
pRSET
pSilencer3.1-H1 hygro
pSK01-T
pSK-CMV(KAN+)
pSP72
pSport1
pSUPER
pTA2
pT-Adv
pEGFP-C-5’
pEGFP-N-5’
pEF-F
pEGFP-C-5’
pEGFP-N-5’
M13F
T7
T7
T7
Stag
pEGFP-N-5’
PETBlueT7UP(太靠前，在
T7前)/ PETBlueUP
pEGFP-C-5’
CMV30
GAL4 AD
GAL4 AD
GAL4 AD/T7
a-FACTOR
T7
M13F/T7
pGEX-4T-5’
GLP1
RVP3
Pinpoint Primer
PIRES-F/T7
5’
pLXSN-F
MalE primer
P5’
MalE primer
M13R(-48)
M13F(-47)
5’AOX/a-factor
5'AOX/PPICZa-F
M13R(-48)
pQE30-F
T7
M13F(-47)
T7
M13F
T7
T7
SP6/M13F(-47)
T7
M13F/T7(优先使用M13F〕
M13F /T7
pECFP-C-3'
pEGFP-N-3’
pEGFP-C-3'
pEGFP-N-3’
M13R
T7 Ter
T7 Ter
T7 Ter
T7 Ter
T7 Ter
T7TER
T7 Ter
pEGFP-N-3’
PETBlueDOWN
pEGFP-C-3'
CMV-24
3'AD
3'AD
3'AD
3'AOX
3'BD
SP6/M13R
pGEX-4T-3’
GLP2
GLP2(RVP4)
SP6
T3/PIRES-R (J40720)
3’
pLXSN-R
M13F(-47)
P3’
M13F(-47)
M13F(-47)
M13R(-48)
3’AOX
3'AOX
无反向引物
pQE-R:
T7TER
M13R
T3
SP6
T7/M13R
T3
T3/M13R
M13R
pTARGET TM
pTO-T7
pTRC99a-c
pTriPLEx2
pTWIN-1
pUC18(19)/118(119)
pUC57
pUCm-T
pVAX1
pWSK29
pXT7
pYES2
pLEXA
pLNCX
pRECEIVER
pSHUTTLE-CMV
pSP64/65
pSTBlue-1
pT7Blue(R)
pRK5
pMSCV
pBABE
pFastBac HT_A (_B, _C)
pFastBac1
pACYCDuet-1 (MCS I)
pDSRED2-N1(KAN+)
pFastBac Dual (MCSI)
pBSN-T
pHISi－1
pRSV-Rev(根据酶切位点选
择不同引物)
EZ-T
pMSCV-hyg
PTHIOHIS (A )
PDEST22
pLXSN
pLEXA
pCDH
PVX
pAcGP67 (_A, _B, _C)
pAD-Track
PLPC
pHAGE-CMV-MCS-IZsGreen
PLENTI-HIS
pDSRED-PRESS-C1
pCR-Blunt
pMT/V5-His_A (_B,_C)
pEASY-T3
pTarget.F(在T7前面〕/T7 M13R
T7
pTRC99C-F
5'pTriPLEx2
T7
M13F(-47)
M13R(-48)/M13R
M13F/T7
T7
T7/M13F/M13F(-47)
T7
T7
pLEXA-F
pLNCX-F
CMV-F
PSHUTTLE-CMV-F
SP6
T7
T7
SP6
MSCV5'
pBABE5’
pFastBac-F
PH-Profor/BaculoVfor
T7TER
pTRC99C-R
T7
T7TER(客户确认再用〕
M13R(-48)
M13F(-47)/M13F
M13R
BGH
T3/M13R/M13R(-48)
SP6
pLEXA-R
pLNCX-R
此载体无反向引物
PSHUTTLE-CMV-R
此载体无反向引物
SP6
M13F(-47)
pSG5-3' or SV40pA-R
MSCV-rev
pBABE3’
pFastBac-R
pFastBac-R
DuetDOWN1
DsRed1-N
pFastBac-R/SV40-pArev
T3
无
ACYCDuetUP 1
pEGFP-N-5
BaculoVfor/PH
T7
PHISi-1
pREPF/M13F
HIV-Rev-NES/pGEX-3'
M13F(-47)
MSCV5'
TRXF(new)
PDEST22-F
pLXSNfw
pLEXA-F
pCDH-EF1
LBA
PH
pAD-Track-F
PLPC-F/CMV-F
pHAGE-CMV-F
CMV-F
pDSRED -C1-F
M13R
MT-Profor
T7/M13F
T7
M13R(-48)
PMSCV-R/
MSCV-rev
M13F
PDEST22-R
pLXSNrv
pLEXA-R
无
LBB
pAcGP67rv
无
PLPC-R
暂没设计
无
pDSRED-C1-R
M13F/T7
BGHrev
SP6/M13R
2.0REV(同
P2
(pCAMBIA 1301))
pLVX-IRES-ZsGreen1 PIRES2-EGFP-F PIRES2-EGFP-R
pDC316
PVAX1
PSHUTTLE-CMV
pSilencer™ 4.1-CMV neo
pDC316F
PLXSN-5
T7/CMV-F
pShuttle-CMV-F
pDC316R
PLXSN-3
BGH
pShuttle-CMV-R
P2
(pCAMBIA 1301)
M13F
PSI-CHECK2-F
CMV-F
PSICHECF-2-R1
pMIR-report
PSI-CHECK2