2024年5月22日发(作者：帛珺俐)

AB972

Genotype:MATα X2180-1B trp10 [rho 0]

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium

bromide-induced rho- derivative of the strain X2180-1B-trp1.

References:Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.

A364A

Genotype:MAT a ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIO

Notes: Used in the systematic sequencing project, the sequence stored in SGD.

References:Hartwell (1967) J. Bacteriol. 93:1662-1670.

XJ24-24a

Genotype:MAT a ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2

Notes: Derived from, but not isogenic to, S288C

References:Strathern et al. (1979) Cell 18:309-319

DC5

Genotype:MAT a leu2-3,112 his3-11,15 can1-11

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

References:Broach et al. (1979) Gene 8:121-133

X2180-1A

Genotype:MAT a SUC2 mal mel gal2 CUP1

Notes:S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were

obtained from sporulated X2180

YNN216

Genotype:MAT a/αura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochre

Notes: Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).

References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.

YPH499

Genotype:MAT a ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1

Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note

that trp1-Δ63, unlike trp1-Δ1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable

2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984;

original source: M. Carlson, Columbia University), which is congenic with S288C.

References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.

2024年5月22日发(作者：帛珺俐)

AB972

Genotype:MATα X2180-1B trp10 [rho 0]

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium

bromide-induced rho- derivative of the strain X2180-1B-trp1.

References:Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.

A364A

Genotype:MAT a ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIO

Notes: Used in the systematic sequencing project, the sequence stored in SGD.

References:Hartwell (1967) J. Bacteriol. 93:1662-1670.

XJ24-24a

Genotype:MAT a ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2

Notes: Derived from, but not isogenic to, S288C

References:Strathern et al. (1979) Cell 18:309-319

DC5

Genotype:MAT a leu2-3,112 his3-11,15 can1-11

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

References:Broach et al. (1979) Gene 8:121-133

X2180-1A

Genotype:MAT a SUC2 mal mel gal2 CUP1

Notes:S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were

obtained from sporulated X2180

YNN216

Genotype:MAT a/αura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochre

Notes: Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).

References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.

YPH499

Genotype:MAT a ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1

Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note

that trp1-Δ63, unlike trp1-Δ1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable

2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984;

original source: M. Carlson, Columbia University), which is congenic with S288C.

References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.