2024年5月22日发(作者:巫马濡霈)
实用标准文案
酿酒酵母表达载体
pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,
酵母载体 pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF pY15TEF
pY16TEF,
酵母基因重组表达载体pUG6, pSH47,
酵母单杂载体pHISi,pLacZi,pHIS2, pGAD424, 酵母双杂交系统
:
酿酒酵母
Y187, 酿酒酵母 AH109 质粒 pGADT7,pGBKT7对照质粒 pGBKT7-53 pGBKT7-lam
pGADT7-T PCL1,
酿酒酵母菌株 INVSc1,YM4271, AH109,
丫
187,
丫
190,
毕赤酵母表达载体
pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZ
a
A,pAO815,pPIC9k-His,pHIL-S1,pPi nk hc , 配套毕赤酵母Pichiapink, 毕赤酵母宿主 X33,
KM71 KM71H GS115
原核表达载体pQE30,31,32,40,60,61,62,等原核表达载体
,
包括pET系列
,
pET-GST pGEX
系列(含 GST标签)
,
pMAL系列
pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis 系列,pQE系
列,pTrc99a,pTrcHis 系列
,
pBV220,221,222,pTXB 系列
,
pLLP-ompA,plN-lll-ompA (分泌型
表达系列),pQBI63 (原核表达带荧光)pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b,
pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b,
pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b,
pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET 44a, pET 49b
pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40
pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C
pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD
HisA,B,C pPi nPoi nt-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF +) ,
pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk 233-3,pACYC184,pBR322,pUC119
pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK
(+) ,pBlueScript SK (-) pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C, 大肠 杆菌冷激
质粒: pColdI pColdII pColdIII pColdTF 原核共表达质粒:
pACYCduet-1,pETduet-1,pCDFduet-1 , pRSFduet-1 Takara 公司大肠杆菌分子伴 侣:
pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大肠杆菌宿主细胞: DH5a JM101
精彩文档
实用标准文案
JM103JM105JM107JM109JM110Top10 Top10F BL21( DE3 HB101 ER2529E2566 C2566
MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21 ( AI)BL21
(DE3 plysS TG1TB1 DH5a( pir) Tuner ( DE3 Bl21 codonplusRIPL Novablue
(DE3 Rosetta Rosetta ( DE3 Rosetta ( DE3 plys Rosetta-gami ( DE3 Rosetta-gamiB
( DE3 , Rosetta-gamiB ( DE3 plysS Orgami ( DE3 OrgamiB
(DE3 HMS174(DE3
植物表达 /RNAi 载体农杆菌 pBI121,pBI121-GFP,pBI101,pBI221,pSN1301,
pUN1301,pRTL2 pRTL2-GFP pRTL2-CFP,pRTL2-RFP, pRTL2-YFP,pCAMBIA300, 1301,
1302,1303,1304,1305, 1381Z, 1391Z,2300, 2301,3300,3301,pCAMBIA
super1300,pCAMBIA super1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RN 载体
pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2, T-D插入 载体(随
机突变体库)pSKI015,pSKI074,真菌ATMT载体 pBIG2RHPH2-GUS-GFP,pBHt1
枯草芽抱杆菌表达载体 pWB980,pHT43,pHP13,pHP43,
pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK,
pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641,
pAL12,pUCX05-bgaB,pHT01 配套菌株 BS 168,WB600,WB800,WB700,
WB800N,1012,FZB42,1A747广宿主质粒 pVLT33
RNAi基因沉默干扰敲除载体 pSilencer1.0 , pSilencer 2.1-U6
hygro , pSilencer 3.1-H1 hygro , pSilencer 3.1-H1 neo, pSilencer 4.1-CMV neo,
ncer 4.1-CMV puro pMIR-REPORT Luciferase RNAi 载体
(oligoengine ) pSuper-puro RNAi 逆转录病毒载体(clontech ) : RNAi-Ready pSIREN-Retro
Q , RNAi-Ready pSIREN-RetroQ-ZsGreen
(
Luciferase shRNA
) RNAi 慢病毒载体(addgene) : pLKO.1
pSile
Annealed Oligonucleotide
哺乳动物表达载体 pcDNA3.1+/-,pcDNA4/HisMax B , pSecTag2 A, pVAX1
pBudCE4.1,pTracer CMV2, pcDNA3.1 (-) /myc-His A , pcDNA6-Myc/His B, pCEP4 pIRES,
pIRESneo pIRES hyg3, pCMV-myc pCMV-HA plRES-puro3 , pIRES-neo3,pCAGGS!乳动
物双杂交系统 pACT pBIND, pACT-MyoD pBIND-Id , pG5luc,pCMV-BD, pCMV-AD,
pBD-p53, pFR-luc,Cytotrap Two-Hybrid
精彩文档
2024年5月22日发(作者:巫马濡霈)
实用标准文案
酿酒酵母表达载体
pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,
酵母载体 pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF pY15TEF
pY16TEF,
酵母基因重组表达载体pUG6, pSH47,
酵母单杂载体pHISi,pLacZi,pHIS2, pGAD424, 酵母双杂交系统
:
酿酒酵母
Y187, 酿酒酵母 AH109 质粒 pGADT7,pGBKT7对照质粒 pGBKT7-53 pGBKT7-lam
pGADT7-T PCL1,
酿酒酵母菌株 INVSc1,YM4271, AH109,
丫
187,
丫
190,
毕赤酵母表达载体
pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZ
a
A,pAO815,pPIC9k-His,pHIL-S1,pPi nk hc , 配套毕赤酵母Pichiapink, 毕赤酵母宿主 X33,
KM71 KM71H GS115
原核表达载体pQE30,31,32,40,60,61,62,等原核表达载体
,
包括pET系列
,
pET-GST pGEX
系列(含 GST标签)
,
pMAL系列
pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis 系列,pQE系
列,pTrc99a,pTrcHis 系列
,
pBV220,221,222,pTXB 系列
,
pLLP-ompA,plN-lll-ompA (分泌型
表达系列),pQBI63 (原核表达带荧光)pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b,
pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b,
pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b,
pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET 44a, pET 49b
pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40
pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C
pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD
HisA,B,C pPi nPoi nt-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF +) ,
pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk 233-3,pACYC184,pBR322,pUC119
pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK
(+) ,pBlueScript SK (-) pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C, 大肠 杆菌冷激
质粒: pColdI pColdII pColdIII pColdTF 原核共表达质粒:
pACYCduet-1,pETduet-1,pCDFduet-1 , pRSFduet-1 Takara 公司大肠杆菌分子伴 侣:
pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大肠杆菌宿主细胞: DH5a JM101
精彩文档
实用标准文案
JM103JM105JM107JM109JM110Top10 Top10F BL21( DE3 HB101 ER2529E2566 C2566
MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21 ( AI)BL21
(DE3 plysS TG1TB1 DH5a( pir) Tuner ( DE3 Bl21 codonplusRIPL Novablue
(DE3 Rosetta Rosetta ( DE3 Rosetta ( DE3 plys Rosetta-gami ( DE3 Rosetta-gamiB
( DE3 , Rosetta-gamiB ( DE3 plysS Orgami ( DE3 OrgamiB
(DE3 HMS174(DE3
植物表达 /RNAi 载体农杆菌 pBI121,pBI121-GFP,pBI101,pBI221,pSN1301,
pUN1301,pRTL2 pRTL2-GFP pRTL2-CFP,pRTL2-RFP, pRTL2-YFP,pCAMBIA300, 1301,
1302,1303,1304,1305, 1381Z, 1391Z,2300, 2301,3300,3301,pCAMBIA
super1300,pCAMBIA super1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RN 载体
pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2, T-D插入 载体(随
机突变体库)pSKI015,pSKI074,真菌ATMT载体 pBIG2RHPH2-GUS-GFP,pBHt1
枯草芽抱杆菌表达载体 pWB980,pHT43,pHP13,pHP43,
pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK,
pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641,
pAL12,pUCX05-bgaB,pHT01 配套菌株 BS 168,WB600,WB800,WB700,
WB800N,1012,FZB42,1A747广宿主质粒 pVLT33
RNAi基因沉默干扰敲除载体 pSilencer1.0 , pSilencer 2.1-U6
hygro , pSilencer 3.1-H1 hygro , pSilencer 3.1-H1 neo, pSilencer 4.1-CMV neo,
ncer 4.1-CMV puro pMIR-REPORT Luciferase RNAi 载体
(oligoengine ) pSuper-puro RNAi 逆转录病毒载体(clontech ) : RNAi-Ready pSIREN-Retro
Q , RNAi-Ready pSIREN-RetroQ-ZsGreen
(
Luciferase shRNA
) RNAi 慢病毒载体(addgene) : pLKO.1
pSile
Annealed Oligonucleotide
哺乳动物表达载体 pcDNA3.1+/-,pcDNA4/HisMax B , pSecTag2 A, pVAX1
pBudCE4.1,pTracer CMV2, pcDNA3.1 (-) /myc-His A , pcDNA6-Myc/His B, pCEP4 pIRES,
pIRESneo pIRES hyg3, pCMV-myc pCMV-HA plRES-puro3 , pIRES-neo3,pCAGGS!乳动
物双杂交系统 pACT pBIND, pACT-MyoD pBIND-Id , pG5luc,pCMV-BD, pCMV-AD,
pBD-p53, pFR-luc,Cytotrap Two-Hybrid
精彩文档