2024年3月10日发(作者：谏水风)

芋螺毒素(Conotoxin-MVIIA )，由海洋腹足纲软体动物芋螺(Conus)的毒液管和毒

囊内壁的毒腺所分泌，由许多单一毒肽组成的鸡尾酒样的混合毒素，主要成分是

一些对不同离子通道及神经受体高专一性的活性多肽化合物。该类毒素对疾病不

同发展阶段和不同部位可能存在不同作用，是临床药物的潜在开发点。

一．

1 mkltcvviva vllltacqli taddsrgtqk hralrsttkl smstrckgkg akcsrlmydc

61 ctgscrsgkc g

1 atgaaactga cgtgtgtggt gatcgtcgcc gtgctgctcc tgacggcctg tcaactcatc

61 acagctgatg actccagagg tacgcagaag catcgtgcct tgaggtcgac caccaaactc

121 tccatgtcga ctcgctgcaa gggtaaagga gcaaaatgtt caaggcttat gtatgactgc

181 tgcaccggtt cttgcagatc aggtaaatgt ggc

二．

Name: Untitled

Conformation: linear

Overhang: five_prime, three_prime, blunt

Minimum Site Length: 5 bases

Maximum Number of Cuts: all

Included: all commercial, prototypes only

Noncutters: AarI, AatII, AbsI, AclI, AcyI, AflII, AjuI, AlfI, AloI, AlwNI, ApaI,

ApaLI, ApoI, ArsI, AscI, AsuII, AvaI, AvaII, AvrII, BaeI, BalI, BamHI, BarI, BbvCI,

BccI, BciVI, BclI, BdaI, BfiI, BglI, BglII, BplI, Bpu10I, BsaAI, BsaBI, BsaXI,

BseMII, BsePI, BseRI, BseSI, BseYI, BsmI, BsmAI, Bsp1407I, BspHI, BspMI, BsrI,

BsrBI, BsrDI, BstEII, BstXI, BtgZI, BtsI, CfrI, ClaI, CspCI, DraII, DraIII, DrdI, EciI,

Eco31I, Eco47III, Eco57I, EcoNI, EcoRI, EcoRII, EcoRV, Esp3I, FalI, FauI, FokI,

FseI, FspAI, HaeII, HgaI, HindIII, HpaI, KpnI, MauBI, MboII, MfeI, MluI, MmeI,

MslI, NaeI, NarI, NcoI, NdeI, NheI, NmeAIII, NotI, NruI, NspI, OliI, PacI, PasI,

PflMI, PfoI, PmaCI, PmeI, PpiI, PpuMI, PshAI, PsiI, PI-PspI, PspXI, PsrI, PstI, PvuI,

RsrII, SacI, SacII, SanDI, SapI, ScaI, PI-SceI, SduI, SexAI, SfiI, SgfI, SgrAI, SgrDI,

SmaI, SnaBI, SpeI, SphI, SrfI, Sse8387I, SspI, StuI, StyI, SwaI, TaqII, TatI, TauI, TfiI,

TsoI, Tsp45I, TspGWI, TspRI, TstI, Tth111I, VspI, XbaI, XcmI, XhoI, XhoII, XmnI

Site

Length

6

Cut

Positions

11

Name

BtrI

Sequence

CACGTC

Overhang Frequency

blunt 1

Name

PvuII

AflIII

AgeI

Cfr10I

EcoP15I

SfaNI

SmlI

AgsI

BsgI

Sequence

CAGCTG

ACRYGT

ACCGGT

RCCGGY

CAGCAG

GCATC

CTYRAG

TTSAA

GTGCAG

Site

Length

6

6

6

6

6

5

6

5

6

6

6

6

6

5

5

5

6

5

6

6

6

6

5

5

Overhang Frequency

blunt

five_prime

five_prime

five_prime

five_prime

five_prime

five_prime

three_prime

three_prime

three_prime

three_prime

three_prime

three_prime

three_prime

three_prime

three_prime

five_prime

five_prime

five_prime

three_prime

three_prime

blunt

five_prime

five_prime

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

2

2

2

2

2

3

3

3

Cut

Positions

64

10

184

184

149

99

99

161

165

47

57

57

66

30

28

16

106, 127

64, 123

105, 126

73, 107

33, 65

52, 107, 128

20, 121, 166

33, 134, 179

Eam1105I GACNNNNNGTC

Eco57MI

GsuI

HaeIV

HphI

Hpy99I

TspDTI

AccI

PleI

SalI

BcgI

Hin4I

HindII

BbvI

TseI

三．分子量： 7617.11

等电点：9.65

CTGRAG

CTGGAG

GAYNNNNNRTC

GGTGA

CGWCG

ATGAA

GTMKAC

GAGTC

GTCGAC

CGANNNNNNTGC

GAYNNNNNVTC

GTYRAC

GCAGC

GCWGC

2024年3月10日发(作者：谏水风)

芋螺毒素(Conotoxin-MVIIA )，由海洋腹足纲软体动物芋螺(Conus)的毒液管和毒

囊内壁的毒腺所分泌，由许多单一毒肽组成的鸡尾酒样的混合毒素，主要成分是

一些对不同离子通道及神经受体高专一性的活性多肽化合物。该类毒素对疾病不

同发展阶段和不同部位可能存在不同作用，是临床药物的潜在开发点。

一．

1 mkltcvviva vllltacqli taddsrgtqk hralrsttkl smstrckgkg akcsrlmydc

61 ctgscrsgkc g

1 atgaaactga cgtgtgtggt gatcgtcgcc gtgctgctcc tgacggcctg tcaactcatc

61 acagctgatg actccagagg tacgcagaag catcgtgcct tgaggtcgac caccaaactc

121 tccatgtcga ctcgctgcaa gggtaaagga gcaaaatgtt caaggcttat gtatgactgc

181 tgcaccggtt cttgcagatc aggtaaatgt ggc

二．

Name: Untitled

Conformation: linear

Overhang: five_prime, three_prime, blunt

Minimum Site Length: 5 bases

Maximum Number of Cuts: all

Included: all commercial, prototypes only

Noncutters: AarI, AatII, AbsI, AclI, AcyI, AflII, AjuI, AlfI, AloI, AlwNI, ApaI,

ApaLI, ApoI, ArsI, AscI, AsuII, AvaI, AvaII, AvrII, BaeI, BalI, BamHI, BarI, BbvCI,

BccI, BciVI, BclI, BdaI, BfiI, BglI, BglII, BplI, Bpu10I, BsaAI, BsaBI, BsaXI,

BseMII, BsePI, BseRI, BseSI, BseYI, BsmI, BsmAI, Bsp1407I, BspHI, BspMI, BsrI,

BsrBI, BsrDI, BstEII, BstXI, BtgZI, BtsI, CfrI, ClaI, CspCI, DraII, DraIII, DrdI, EciI,

Eco31I, Eco47III, Eco57I, EcoNI, EcoRI, EcoRII, EcoRV, Esp3I, FalI, FauI, FokI,

FseI, FspAI, HaeII, HgaI, HindIII, HpaI, KpnI, MauBI, MboII, MfeI, MluI, MmeI,

MslI, NaeI, NarI, NcoI, NdeI, NheI, NmeAIII, NotI, NruI, NspI, OliI, PacI, PasI,

PflMI, PfoI, PmaCI, PmeI, PpiI, PpuMI, PshAI, PsiI, PI-PspI, PspXI, PsrI, PstI, PvuI,

RsrII, SacI, SacII, SanDI, SapI, ScaI, PI-SceI, SduI, SexAI, SfiI, SgfI, SgrAI, SgrDI,

SmaI, SnaBI, SpeI, SphI, SrfI, Sse8387I, SspI, StuI, StyI, SwaI, TaqII, TatI, TauI, TfiI,

TsoI, Tsp45I, TspGWI, TspRI, TstI, Tth111I, VspI, XbaI, XcmI, XhoI, XhoII, XmnI

Site

Length

6

Cut

Positions

11

Name

BtrI

Sequence

CACGTC

Overhang Frequency

blunt 1

Name

PvuII

AflIII

AgeI

Cfr10I

EcoP15I

SfaNI

SmlI

AgsI

BsgI

Sequence

CAGCTG

ACRYGT

ACCGGT

RCCGGY

CAGCAG

GCATC

CTYRAG

TTSAA

GTGCAG

Site

Length

6

6

6

6

6

5

6

5

6

6

6

6

6

5

5

5

6

5

6

6

6

6

5

5

Overhang Frequency

blunt

five_prime

five_prime

five_prime

five_prime

five_prime

five_prime

three_prime

three_prime

three_prime

three_prime

three_prime

three_prime

three_prime

three_prime

three_prime

five_prime

five_prime

five_prime

three_prime

three_prime

blunt

five_prime

five_prime

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

2

2

2

2

2

3

3

3

Cut

Positions

64

10

184

184

149

99

99

161

165

47

57

57

66

30

28

16

106, 127

64, 123

105, 126

73, 107

33, 65

52, 107, 128

20, 121, 166

33, 134, 179

Eam1105I GACNNNNNGTC

Eco57MI

GsuI

HaeIV

HphI

Hpy99I

TspDTI

AccI

PleI

SalI

BcgI

Hin4I

HindII

BbvI

TseI

三．分子量： 7617.11

等电点：9.65

CTGRAG

CTGGAG

GAYNNNNNRTC

GGTGA

CGWCG

ATGAA

GTMKAC

GAGTC

GTCGAC

CGANNNNNNTGC

GAYNNNNNVTC

GTYRAC

GCAGC

GCWGC