2024年3月10日发(作者：过星腾)

一、写出你拟研发的基因工程药物的cDNA序列和蛋白质序列（可自由选择有应

用价值的靶标基因）。

研发的基因工程药物名称：重组人GCSF（

CSF3

）

cDNA序列：

1 atggctggac ctgccaccca gagccccatg aagctgatgg ccctgcagct gctgctgtgg

61 cacagtgcac tctggacagt gcaggaagcc acccccctgg gccctgccag ctccctgccc

121 cagagcttcc tgctcaagtg cttagagcaa gtgaggaaga tccagggcga tggcgcagcg

181 ctccaggaga agctgtgtgc cacctacaag ctgtgccacc ccgaggagct ggtgctgctc

241 ggacactctc tgggcatccc ctgggctccc ctgagcagct gccccagcca ggccctgcag

301 ctggcaggct gcttgagcca actccatagc ggccttttcc tctaccaggg gctcctgcag

361 gccctggaag ggatctcccc cgagttgggt cccaccttgg acacactgca gctggacgtc

421 gccgactttg ccaccaccat ctggcagcag atggaagaac tgggaatggc ccctgccctg

481 cagcccaccc agggtgccat gccggccttc gcctctgctt tccagcgccg ggcaggaggg

541 gtcctagttg cctcccatct gcagagcttc ctggaggtgt cgtaccgcgt tctacgccac

601 cttgcccagc cc

蛋白质序列：

1 magpatqspm klmalqlllw hsalwtvqea tplgpasslp qsfllkcleq vrkiqgdgaa

61 lqeklcatyk lchpeelvll ghslgipwap lsscpsqalq lagclsqlhs glflyqgllq

121 alegispelg ptldtlqldv adfattiwqq meelgmapal qptqgampaf asafqrragg

181 vlvashlqsf levsyrvlrh laqp

二、分析该cDNA序列的限制性内切酶位点。

Name:GCSF

Conformation: linear

Overhang: five_prime, three_prime, blunt

Minimum Site Length: 5 bases

Maximum Number of Cuts: 1

Included: all commercial, prototypes only

Noncutters: AarI, AbsI, AccI, AclI, AflII, AflIII, AgeI, AgsI, AjuI, AlfI, AloI, ApoI, ArsI,

AscI, AsuII, AvrII, BaeI, BalI, BamHI, BarI, BbvCI, BcgI, BciVI, BclI, BdaI, BglII, BplI,

BsaAI, BsaBI, BsePI, BsmI, BsmAI, Bsp1407I, BspHI, BsrBI, BsrDI, BstEII, BtrI, CfrI,

ClaI, CspCI, DraIII, DrdI, Eam1105I, EciI, Eco31I, Eco57I, EcoRI, EcoRV, Esp3I,

FalI, FauI, FseI, FspAI, HaeIV, HgaI, Hin4I, HindII, HindIII, HpaI, HphI, KpnI,

MauBI, MfeI, MluI, MmeI, MslI, NarI, NcoI, NdeI, NheI, NmeAIII, NotI, NruI, NspI,

OliI, PacI, PleI, PmaCI, PmeI, PpiI, PshAI, PsiI, PI-PspI, PspXI, PsrI, PvuI, RsrII, SacI,

SacII, SalI, SapI, ScaI, PI-SceI, SexAI, SfiI, SgfI, SgrAI, SgrDI, SmaI, SnaBI, SpeI, SphI,

SrfI, SspI, StuI, SwaI, TaqII, TatI, TfiI, TsoI, Tsp45I, TspGWI, Tth111I, VspI, XbaI,

XhoI, XmnI

Name

Eco47III

NaeI

AcyI

ApaLI

Bpu10I

Sequence

AGCGCT

GCCGGC

GRCGYC

GTGCAC

CCTNAGC

Site

Length

6

6

6

6

6

Overhang

blunt

blunt

five_prime

five_prime

five_prime

Frequency

1

1

1

1

1

Cut Positions

179

503

416

65

271

2024年3月10日发(作者：过星腾)

一、写出你拟研发的基因工程药物的cDNA序列和蛋白质序列（可自由选择有应

用价值的靶标基因）。

研发的基因工程药物名称：重组人GCSF（

CSF3

）

cDNA序列：

1 atggctggac ctgccaccca gagccccatg aagctgatgg ccctgcagct gctgctgtgg

61 cacagtgcac tctggacagt gcaggaagcc acccccctgg gccctgccag ctccctgccc

121 cagagcttcc tgctcaagtg cttagagcaa gtgaggaaga tccagggcga tggcgcagcg

181 ctccaggaga agctgtgtgc cacctacaag ctgtgccacc ccgaggagct ggtgctgctc

241 ggacactctc tgggcatccc ctgggctccc ctgagcagct gccccagcca ggccctgcag

301 ctggcaggct gcttgagcca actccatagc ggccttttcc tctaccaggg gctcctgcag

361 gccctggaag ggatctcccc cgagttgggt cccaccttgg acacactgca gctggacgtc

421 gccgactttg ccaccaccat ctggcagcag atggaagaac tgggaatggc ccctgccctg

481 cagcccaccc agggtgccat gccggccttc gcctctgctt tccagcgccg ggcaggaggg

541 gtcctagttg cctcccatct gcagagcttc ctggaggtgt cgtaccgcgt tctacgccac

601 cttgcccagc cc

蛋白质序列：

1 magpatqspm klmalqlllw hsalwtvqea tplgpasslp qsfllkcleq vrkiqgdgaa

61 lqeklcatyk lchpeelvll ghslgipwap lsscpsqalq lagclsqlhs glflyqgllq

121 alegispelg ptldtlqldv adfattiwqq meelgmapal qptqgampaf asafqrragg

181 vlvashlqsf levsyrvlrh laqp

二、分析该cDNA序列的限制性内切酶位点。

Name:GCSF

Conformation: linear

Overhang: five_prime, three_prime, blunt

Minimum Site Length: 5 bases

Maximum Number of Cuts: 1

Included: all commercial, prototypes only

Noncutters: AarI, AbsI, AccI, AclI, AflII, AflIII, AgeI, AgsI, AjuI, AlfI, AloI, ApoI, ArsI,

AscI, AsuII, AvrII, BaeI, BalI, BamHI, BarI, BbvCI, BcgI, BciVI, BclI, BdaI, BglII, BplI,

BsaAI, BsaBI, BsePI, BsmI, BsmAI, Bsp1407I, BspHI, BsrBI, BsrDI, BstEII, BtrI, CfrI,

ClaI, CspCI, DraIII, DrdI, Eam1105I, EciI, Eco31I, Eco57I, EcoRI, EcoRV, Esp3I,

FalI, FauI, FseI, FspAI, HaeIV, HgaI, Hin4I, HindII, HindIII, HpaI, HphI, KpnI,

MauBI, MfeI, MluI, MmeI, MslI, NarI, NcoI, NdeI, NheI, NmeAIII, NotI, NruI, NspI,

OliI, PacI, PleI, PmaCI, PmeI, PpiI, PshAI, PsiI, PI-PspI, PspXI, PsrI, PvuI, RsrII, SacI,

SacII, SalI, SapI, ScaI, PI-SceI, SexAI, SfiI, SgfI, SgrAI, SgrDI, SmaI, SnaBI, SpeI, SphI,

SrfI, SspI, StuI, SwaI, TaqII, TatI, TfiI, TsoI, Tsp45I, TspGWI, Tth111I, VspI, XbaI,

XhoI, XmnI

Name

Eco47III

NaeI

AcyI

ApaLI

Bpu10I

Sequence

AGCGCT

GCCGGC

GRCGYC

GTGCAC

CCTNAGC

Site

Length

6

6

6

6

6

Overhang

blunt

blunt

five_prime

five_prime

five_prime

Frequency

1

1

1

1

1

Cut Positions

179

503

416

65

271