2024年3月17日发(作者：老文静)
Mitochondrial DNA
The Journal of DNA Mapping, Sequencing, and Analysis
ISSN: 1940-1736 (Print) 1940-1744 (Online) Journal homepage: /loi/imdn20
The complete mitochondrial genome sequence
of Sinogastromyzon sichangensis (Cypriniformes,
Balitoridae)
Dan Yu, Rong He & Qiongying Tang
To cite this article: Dan Yu, Rong He & Qiongying Tang (2015) The complete mitochondrial
genome sequence of Sinogastromyzon sichangensis (Cypriniformes, Balitoridae),
Mitochondrial DNA, 26:6, 859-860, DOI: 10.3109/19401736.2013.861430
To link to this article: /10.3109/19401736.2013.861430
Published online: 10 Jan 2014.
Submit your article to this journal
Article views: 18
View related articles
View Crossmark data
Citing articles: 3 View citing articles
Full Terms & Conditions of access and use can be found at
/action/journalInformation?journalCode=imdn20
Download by: [University North Carolina - Chapel Hill]Date: 27 October 2015, At: 05:39
/mdn
ISSN:1940-1736(print),1940-1744(electronic)
MitochondrialDNA,2015;26(6):859–860
!
:10.3109/19401736.2013.861430
MITOGENOMEANNOUNCEMENT
ThecompletemitochondrialgenomesequenceofSinogastromyzon
sichangensis(Cypriniformes,Balitoridae)
DanYu
1
,RongHe
2
,andQiongyingTang
1
1
TheKeyLaboratoryofAquaticBiodiversityandConservationofChineseAcademyofSciences,InstituteofHydrobiology,ChineseAcademyof
Sciences,Wuhan,nd
2
DepartmentofFisheries,SouthwestUniversity,Rongchang,Chongqing,
Abstract
D
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w
n
l
o
a
d
e
d
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y
[
U
n
i
v
e
r
s
i
t
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h
C
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Keywords
Balitoridae,mitogenome,phylogenetic
position,Sinogastromyzonsichangensis
History
Received2October2013
Revised8October2013
Accepted24October2013
Publishedonline10January2014
Sinogastromyzonsichangensis,belongingtothefamilyBalitoridaeinCypriniformes,isendemic
study,thecompletemitochondrial
ochondrial
gensisissimilartothoseofthetypicalvertebrates,16,567bpinlength,
including13protein-codinggenes,tworibosomalRNAgenes,22transferRNAgenesandone
non-codingcontrolregion(D-loop).gensiswascharacterizedbyone
termination-associatedsequenceandsevenconservedsequenceblocks(CSB-F,CSB-E,CSB-D,
CSB-BandCSBI-III).gensiscouldcontributetoestimate
rinvestigationswithmore
Sinogastromyzonspeciesandotherbalitoridfishesincludedshouldbeperformedtobetter
understandtheevolutionaryhistoryofthisuniquegroupoffisheswhichcansuccessfullyadapt
tomountaintorrents.
TheBalitoridae(referringtohillstreamloaches)isauniquegroup
ofCypriniformes,theirpairedfinsenlargedwithadhesivepadson
ventralsurface,whichmakethembesuccessfullyadaptiveto
tlyonlyfivebalitoridspeciesmitogen-
omesaresequenced,withonespeciesSinogastromyzonpuliensis
fromSinogastromyzon,thegenuswhichhas21validspecies
describedsofar(Froese&Pauly,2013).Thespecies
Sinogastromyzonsichangensis(Cypriniformes,Balitoridae)is
endemictotheUpperYangtzeRiverandtheQingRiver(Yue,
2000).Inthisstudy,wesequencedthecompletemitochondrial
gensiswiththeGenBankaccessionnumber
gensisindividualswerecollected
fromGuizhouprovinceofChina.
gensismitogenomesequencewas
16,567bpinlength,andwascomposedof13protein-coding
genes(cytb,ATP6,ATP8,COI-III,ND1-6andND4L),tworRNA
genes(12Sand16SrRNA),22tRNAgenesandonenon-coding
controlregion(CR),mostofwhichwereencodedontheH-strand,
exceptforND6andeighttRNAgenes(tRNA
Gln
,tRNA
Ala
,
tRNA
Asn
,tRNA
Cys
,tRNA
Tyr
,tRNA
Ser
,tRNA
Glu
andtRNA
Pro
;
Table1).Thesefeaturesweresimilartomitogenomeof
thetypicalvertebrates(Heetal.,2011;Pengetal.,2006;
Tangetal.,2013;Wangetal.,2012).Thenucleotidecomposition
gensismitogenomewasA–30.3%,T–25.2%,
C–27.9%andG–16.6%,withaslightAþTrichfeature
(55.5%).Almostallofthe13protein-codinggenesshowed
Correspondence:,TheKeyLaboratoryofAquaticBiodiversity
andConservationofChineseAcademyofSciences,Instituteof
Hydrobiology,ChineseAcademyofSciences,Wuhan430072,P.R.
:+:+86-27-68780065.E-mail:
tangqy@
gensis.
Positionnumber
FeatureStartStop
Codon
SizeIntergenic
(bp)StartStopnucleotide*Strandy
H
H
H
H
H
H
H
L
H
H
H
L
L
L
L
H
L
H
H
H
H
H
H
H
H
H
H
(continued)
tRNA
Phe
1
12SrRNA70
Val
tRNA1023
16SrRNA1095
Leu(UUR)
tRNA
2776
ND12852
Ile
tRNA
3830
tRNA
Gln
3900
Met
tRNA
3972
ND24041
Trp
tRNA
5086
tRNA
Ala
5158
Asn
tRNA5228
rep_origin5301
tRNA
Cys
5332
tRNA
Tyr
5399
COI5469
Ser(UCN)
tRNA
7021
tRNA
Asp
7094
COII7181
tRNA
Lys
7872
ATPase87949
ATPase68107
COIII8790
Gly
tRNA
9575
ND39648
Arg
tRNA
9997
ND4L10,067
6969
1022953
109472
27751681
285075
3826975ATGTAA
390172
397071
404069
50871047ATGTAG
515570
522669
530073
533131
539766
546769
70191551GTGTAA
709171
716673
7871691ATGT
794776
8116168ATGTAA
8790684ATGTAA
9575786ATGTAA
964773
9998351ATGTAG
10,06670
10,363297ATGTAA
–1
–3
þ2
–1
þ2
–2
–1
–1
–1
–1
–2
–14
–1
þ10
þ1
þ1
þ2
.
ued
PositionnumberCodon
SizeIntergenic
FeatureStartStop(bp)StartStopnucleotide*Strandy
ND410,35711,7391383ATGTAGþ7H
tRNA
His
0þ1H
tRNA
Ser(AGN)
11,80811,87770þ1H
tRNA
Leu(CUN)
11,87811,95073H
ND511,95113,7891839ATGTAAH
ND613,78614,307522ATGTAAþ4L
tRNA
Glu
14,30814,37669L
Cytb14,38315,5231141ATGT–6H
tRNA
Thr
15,52415,59572H
tRNA
Pro
15,59715,66670–1L
D-Loop15,66716,567901H
*Àandþrepresenttheintervalandoverlapnucleotidebetweentwo
adjacentgenes,respectively.
yLandHrefertolightandheavystrands,respectively.
theregularinitiationcodonATGwiththeonlyexceptionof
protein-codinggenes
sharedthetypicalstopcodons(TAAforeightgenesandTAG
forthreegenes),andtheremainingtwo(COIIandcytb)
-loop
was901bplong,anditsbasecompositionwasA–34.2%,
T–32.5%,C–18.9%andG–14.4%,reflectingastrongrich
featureinAandT(AþT¼66.7%).Thetermination-associated
sequencewasidentified,andtwomotifs,‘‘TACAT’’andits
revertedandcomplementedsequence‘‘ATGTA’’couldbe
found;fourcentralconservedsequences(CSB-F,CSB-E,CSB-
BandCSB-D)werealsoidentifiedbyfollowingLiu(2002);three
conservedsequenceblocks(CSBI–III)couldbeeasilyidentified,
whichwerethoughttobeinvolvedinpositioningRNA
polymerasebothfortranscriptionandforprimingreplication
(Clayton,1991;Shadel&Clayton,1997).
Currentlysomedetailedstudiesonthegenus
Sinogastromyzon’staxonomyandbiologyhavebeencarriedout,
MitochondrialDNA,2015;26(6):859–860
butmanyotheraspectssuchasgeneticsandmolecular
rkprovides
fundamentaldataforfurtherphylogeography,populationgenetics,
andconversationgeneticsstudiesonthisbalitoridspecies.
Acknowledgements
TheauthorsthankDeQingTanandYuyuXiongfortheirhelpin
appreciateSenHeforhisassistancewiththe
laboratorywork.
Declarationofinterest
Theauthorsaloneareresponsibleforthecontentandwritingofthepaper.
ThisworkwassupportedbythegrantsfromtheNationalNaturalScience
FoundationofChina(GrantNo.31272306and31061160185).
References
ClaytonDA.(1991).NucleargadgetsinmitochondrialDNAreplication
BiochemSci16:107–11.
FroeseR,PaulyD.(2013).bleat:,
version(AccessedAugust2013).
HeAY,LuoYJ,YangH,LiuLP,LiSF,WangCH.(2011).Complete
mitochondrialDNAsequencesoftheNiletilapia(Oreochromis
niloticus)andBluetilapia(Oreochromisaureus):Genomecharacter-
lRep38:2015–21.
LiuHZ.(2002).Structureandevolutionoffishmitochondrialcontrol
region,tSci12:266–70.
PengZG,WangJ,HeSP.(2006).Thecompletemitochondrialgenomeof
thehelmetcatfishCranoglanisbouderius(Siluriformes:
Cranoglanididae)376:
290–7.
ShadelGS,ClaytonDA.(1997).MitochondrialDNAmaintenancein
vBiochem66:409–35.
TangQY,HuangY,WangJJ,HuangJ,WangZJ,PengZG.(2013).The
completemitochondrialgenomesequenceofTriplophysableekeri
(Teleostei,Balitoridae,Nemacheilinae).MitochondrialDNA24:25–7.
WangJJ,TangQY,WangZJ,ZhangYG,WuQ,PengZG.(2012).The
completemitogenomesequenceofacaveloachTriplophysarosa
(Teleostei,Balitoridae,Nemacheilinae).MitochondrialDNA23:
366–8.
YuePQ.(2000).g:
SciencesPress.
D
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n
l
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[
U
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v
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s
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N
o
r
t
h
C
a
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a
p
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H
i
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2024年3月17日发(作者：老文静)
Mitochondrial DNA
The Journal of DNA Mapping, Sequencing, and Analysis
ISSN: 1940-1736 (Print) 1940-1744 (Online) Journal homepage: /loi/imdn20
The complete mitochondrial genome sequence
of Sinogastromyzon sichangensis (Cypriniformes,
Balitoridae)
Dan Yu, Rong He & Qiongying Tang
To cite this article: Dan Yu, Rong He & Qiongying Tang (2015) The complete mitochondrial
genome sequence of Sinogastromyzon sichangensis (Cypriniformes, Balitoridae),
Mitochondrial DNA, 26:6, 859-860, DOI: 10.3109/19401736.2013.861430
To link to this article: /10.3109/19401736.2013.861430
Published online: 10 Jan 2014.
Submit your article to this journal
Article views: 18
View related articles
View Crossmark data
Citing articles: 3 View citing articles
Full Terms & Conditions of access and use can be found at
/action/journalInformation?journalCode=imdn20
Download by: [University North Carolina - Chapel Hill]Date: 27 October 2015, At: 05:39
/mdn
ISSN:1940-1736(print),1940-1744(electronic)
MitochondrialDNA,2015;26(6):859–860
!
:10.3109/19401736.2013.861430
MITOGENOMEANNOUNCEMENT
ThecompletemitochondrialgenomesequenceofSinogastromyzon
sichangensis(Cypriniformes,Balitoridae)
DanYu
1
,RongHe
2
,andQiongyingTang
1
1
TheKeyLaboratoryofAquaticBiodiversityandConservationofChineseAcademyofSciences,InstituteofHydrobiology,ChineseAcademyof
Sciences,Wuhan,nd
2
DepartmentofFisheries,SouthwestUniversity,Rongchang,Chongqing,
Abstract
D
o
w
n
l
o
a
d
e
d
b
y
[
U
n
i
v
e
r
s
i
t
y
N
o
r
t
h
C
a
r
o
l
i
n
a
-
C
h
a
p
e
l
H
i
l
l
]
a
t
0
5
:
3
9
2
7
O
c
t
o
b
e
r
2
0
1
5
Keywords
Balitoridae,mitogenome,phylogenetic
position,Sinogastromyzonsichangensis
History
Received2October2013
Revised8October2013
Accepted24October2013
Publishedonline10January2014
Sinogastromyzonsichangensis,belongingtothefamilyBalitoridaeinCypriniformes,isendemic
study,thecompletemitochondrial
ochondrial
gensisissimilartothoseofthetypicalvertebrates,16,567bpinlength,
including13protein-codinggenes,tworibosomalRNAgenes,22transferRNAgenesandone
non-codingcontrolregion(D-loop).gensiswascharacterizedbyone
termination-associatedsequenceandsevenconservedsequenceblocks(CSB-F,CSB-E,CSB-D,
CSB-BandCSBI-III).gensiscouldcontributetoestimate
rinvestigationswithmore
Sinogastromyzonspeciesandotherbalitoridfishesincludedshouldbeperformedtobetter
understandtheevolutionaryhistoryofthisuniquegroupoffisheswhichcansuccessfullyadapt
tomountaintorrents.
TheBalitoridae(referringtohillstreamloaches)isauniquegroup
ofCypriniformes,theirpairedfinsenlargedwithadhesivepadson
ventralsurface,whichmakethembesuccessfullyadaptiveto
tlyonlyfivebalitoridspeciesmitogen-
omesaresequenced,withonespeciesSinogastromyzonpuliensis
fromSinogastromyzon,thegenuswhichhas21validspecies
describedsofar(Froese&Pauly,2013).Thespecies
Sinogastromyzonsichangensis(Cypriniformes,Balitoridae)is
endemictotheUpperYangtzeRiverandtheQingRiver(Yue,
2000).Inthisstudy,wesequencedthecompletemitochondrial
gensiswiththeGenBankaccessionnumber
gensisindividualswerecollected
fromGuizhouprovinceofChina.
gensismitogenomesequencewas
16,567bpinlength,andwascomposedof13protein-coding
genes(cytb,ATP6,ATP8,COI-III,ND1-6andND4L),tworRNA
genes(12Sand16SrRNA),22tRNAgenesandonenon-coding
controlregion(CR),mostofwhichwereencodedontheH-strand,
exceptforND6andeighttRNAgenes(tRNA
Gln
,tRNA
Ala
,
tRNA
Asn
,tRNA
Cys
,tRNA
Tyr
,tRNA
Ser
,tRNA
Glu
andtRNA
Pro
;
Table1).Thesefeaturesweresimilartomitogenomeof
thetypicalvertebrates(Heetal.,2011;Pengetal.,2006;
Tangetal.,2013;Wangetal.,2012).Thenucleotidecomposition
gensismitogenomewasA–30.3%,T–25.2%,
C–27.9%andG–16.6%,withaslightAþTrichfeature
(55.5%).Almostallofthe13protein-codinggenesshowed
Correspondence:,TheKeyLaboratoryofAquaticBiodiversity
andConservationofChineseAcademyofSciences,Instituteof
Hydrobiology,ChineseAcademyofSciences,Wuhan430072,P.R.
:+:+86-27-68780065.E-mail:
tangqy@
gensis.
Positionnumber
FeatureStartStop
Codon
SizeIntergenic
(bp)StartStopnucleotide*Strandy
H
H
H
H
H
H
H
L
H
H
H
L
L
L
L
H
L
H
H
H
H
H
H
H
H
H
H
(continued)
tRNA
Phe
1
12SrRNA70
Val
tRNA1023
16SrRNA1095
Leu(UUR)
tRNA
2776
ND12852
Ile
tRNA
3830
tRNA
Gln
3900
Met
tRNA
3972
ND24041
Trp
tRNA
5086
tRNA
Ala
5158
Asn
tRNA5228
rep_origin5301
tRNA
Cys
5332
tRNA
Tyr
5399
COI5469
Ser(UCN)
tRNA
7021
tRNA
Asp
7094
COII7181
tRNA
Lys
7872
ATPase87949
ATPase68107
COIII8790
Gly
tRNA
9575
ND39648
Arg
tRNA
9997
ND4L10,067
6969
1022953
109472
27751681
285075
3826975ATGTAA
390172
397071
404069
50871047ATGTAG
515570
522669
530073
533131
539766
546769
70191551GTGTAA
709171
716673
7871691ATGT
794776
8116168ATGTAA
8790684ATGTAA
9575786ATGTAA
964773
9998351ATGTAG
10,06670
10,363297ATGTAA
–1
–3
þ2
–1
þ2
–2
–1
–1
–1
–1
–2
–14
–1
þ10
þ1
þ1
þ2
.
ued
PositionnumberCodon
SizeIntergenic
FeatureStartStop(bp)StartStopnucleotide*Strandy
ND410,35711,7391383ATGTAGþ7H
tRNA
His
0þ1H
tRNA
Ser(AGN)
11,80811,87770þ1H
tRNA
Leu(CUN)
11,87811,95073H
ND511,95113,7891839ATGTAAH
ND613,78614,307522ATGTAAþ4L
tRNA
Glu
14,30814,37669L
Cytb14,38315,5231141ATGT–6H
tRNA
Thr
15,52415,59572H
tRNA
Pro
15,59715,66670–1L
D-Loop15,66716,567901H
*Àandþrepresenttheintervalandoverlapnucleotidebetweentwo
adjacentgenes,respectively.
yLandHrefertolightandheavystrands,respectively.
theregularinitiationcodonATGwiththeonlyexceptionof
protein-codinggenes
sharedthetypicalstopcodons(TAAforeightgenesandTAG
forthreegenes),andtheremainingtwo(COIIandcytb)
-loop
was901bplong,anditsbasecompositionwasA–34.2%,
T–32.5%,C–18.9%andG–14.4%,reflectingastrongrich
featureinAandT(AþT¼66.7%).Thetermination-associated
sequencewasidentified,andtwomotifs,‘‘TACAT’’andits
revertedandcomplementedsequence‘‘ATGTA’’couldbe
found;fourcentralconservedsequences(CSB-F,CSB-E,CSB-
BandCSB-D)werealsoidentifiedbyfollowingLiu(2002);three
conservedsequenceblocks(CSBI–III)couldbeeasilyidentified,
whichwerethoughttobeinvolvedinpositioningRNA
polymerasebothfortranscriptionandforprimingreplication
(Clayton,1991;Shadel&Clayton,1997).
Currentlysomedetailedstudiesonthegenus
Sinogastromyzon’staxonomyandbiologyhavebeencarriedout,
MitochondrialDNA,2015;26(6):859–860
butmanyotheraspectssuchasgeneticsandmolecular
rkprovides
fundamentaldataforfurtherphylogeography,populationgenetics,
andconversationgeneticsstudiesonthisbalitoridspecies.
Acknowledgements
TheauthorsthankDeQingTanandYuyuXiongfortheirhelpin
appreciateSenHeforhisassistancewiththe
laboratorywork.
Declarationofinterest
Theauthorsaloneareresponsibleforthecontentandwritingofthepaper.
ThisworkwassupportedbythegrantsfromtheNationalNaturalScience
FoundationofChina(GrantNo.31272306and31061160185).
References
ClaytonDA.(1991).NucleargadgetsinmitochondrialDNAreplication
BiochemSci16:107–11.
FroeseR,PaulyD.(2013).bleat:,
version(AccessedAugust2013).
HeAY,LuoYJ,YangH,LiuLP,LiSF,WangCH.(2011).Complete
mitochondrialDNAsequencesoftheNiletilapia(Oreochromis
niloticus)andBluetilapia(Oreochromisaureus):Genomecharacter-
lRep38:2015–21.
LiuHZ.(2002).Structureandevolutionoffishmitochondrialcontrol
region,tSci12:266–70.
PengZG,WangJ,HeSP.(2006).Thecompletemitochondrialgenomeof
thehelmetcatfishCranoglanisbouderius(Siluriformes:
Cranoglanididae)376:
290–7.
ShadelGS,ClaytonDA.(1997).MitochondrialDNAmaintenancein
vBiochem66:409–35.
TangQY,HuangY,WangJJ,HuangJ,WangZJ,PengZG.(2013).The
completemitochondrialgenomesequenceofTriplophysableekeri
(Teleostei,Balitoridae,Nemacheilinae).MitochondrialDNA24:25–7.
WangJJ,TangQY,WangZJ,ZhangYG,WuQ,PengZG.(2012).The
completemitogenomesequenceofacaveloachTriplophysarosa
(Teleostei,Balitoridae,Nemacheilinae).MitochondrialDNA23:
366–8.
YuePQ.(2000).g:
SciencesPress.
D
o
w
n
l
o
a
d
e
d
b
y
[
U
n
i
v
e
r
s
i
t
y
N
o
r
t
h
C
a
r
o
l
i
n
a
-
C
h
a
p
e
l
H
i
l
l
]
a
t
0
5
:
3
9
2
7
O
c
t
o
b
e
r
2
0
1
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