2024年5月30日发(作者：雪玉轩)
Enzymes
Serineproteases（丝氨酸蛋白酶）
α-chymotrypsin(胰凝乳蛋白酶，糜
蛋白酶)
Trypsin (胰朊酶，胰蛋白酶)
Pancreatic elastase (胰弹性蛋白酶)
Thrombin(凝血酶)
Plasmin(胞质素，胞浆素)
Prolyl oligopeptidase,or prolylendopeptidase
Plasma kallikrein (血浆激肽释放酶)
E.C. Number
E.C.3.4.21.
E.C.3.4.21.1
E.C.3.4.21.4
E.C.3.4.21.36
E.C.3.4.21.5
E.C.3.4.21.7
E.C.3.4.21.26
E.C.3.4.21.34
Cleavage sites
Tyr-|-Xaa,Trp-|-Xaa, Phe-|-Xaa, and
alsoLeu-|-Xaa,Met-|-Xaa,
Arg-|-Xaa, Lys-|-Xaa,
Ala-|-Xaa,andalsoGly-|-Xaa,
Val-|-Xaa, Ser-|-Xaa,
Arg-|-Gly,
Lys-|-Xaa,>Arg-|-Xaa
Arg-|-Xaa, Lys-|-Xaa,including
Arg-|-Ser,Lys-|-Arg,
Val-|-Xaa, Ala-|-Xaa,
Leukocyteelastase(白细胞弹性蛋白
酶),orneutrophilelastase(中性白细胞弹性蛋白
酶),orlysosomalelastase(溶酶体弹性蛋白酶)
Cysteine proteases（半胱氨酸蛋白酶）
Cathepsin B (组织蛋白酶)
E.C.3.4.21.37
E.C.3.4.22.
E.C.3.4.22.1
Arg-Arg-|-Xaa,and also Leu-|-Xaa,
Ala-|-Xaa, Phe-|-Xaa, Trp-|-Xaa,
Arg-|-Xaa including Arg-|-Pro,but not
Lys-|-Xaa
Met-|-Xaa,Tyr-|-Xaa and
Arg-|-Xaa(with LeuorVal as the P2
residue)
Preferentially Phe-|-Xaa, Tyr-|-Xaa,
And also Leu-|-Xaa,and Trp-|-Xaa,
ideally with Xaa= Phe,Trp,orTyr
Preferentially Phe-|-Xaa, Tyr-|-Xaa,
And also Leu-|-Xaa,ideally with Xaa
≠AlaorVal
Xaa-|-Tyr,Xaa-|-Phe, Xaa-|-Trp,and
Xaa-|-Leu,
Clostripain(梭菌蛋白酶),or endoproteinase
E.C.3.4.22.8
Arg-C
Calpain(钙激活中性蛋白酶)-1,or μ-calpain
E.C.3.4.22.52
Aspartic acid proteases (天冬氨酸蛋白酶)
Pepsin(胃朊酶，胃蛋白酶)
E.C.3.4.23.
E.C.3.4.23.1
Cathepsin(组织蛋白酶)D
E.C.3.4.23.5
Metalloproteases （金属蛋白酶）
E.C.3.4.24.
Neprilysin(脑啡肽酶),or enkephalinase(脑啡肽
E.C.3.4.24.11
酶),or neutralendopeptidase
Thimet oligopeptidase(甲拌磷寡肽 酶),or
E.C.3.4.24.15
endo-oligopeptidase A,or endopeptidase(肽链
内切 酶)24.15,or pz-peptidease(肠促胰 酶素)
Aminopeptidases （氨肽酶）
Leucyl-aminopeptidase
E.C.3.4.11.
E.C.3.4.11.1
Xaa-|-Arg, Xaa-|-Ser,Xaa-|-Ile,
Xaa-|-Ala, Xaa-|-Gly,
N-term
Preferentially Leu-|-Xaa,but not
Arg-|-Xaa,andLys-|-Xaa,
Preferentially Ala-|-Xaa,and
Tyr-|-Xaa,if Yaa-Pro-|-Xaa in term with
Yaa=Ala,Val,Leu,Ile,Phe,Tyr or Trp,then
the dipeptide Yaa-Pro could be
released
Arg-|-Xaa, Lys-|-Xaa,
Glu-|-Xaa>>Asp-|-Xaa,
N-term(di-and tripeptides)
Aminopeptidase M or N,or alanyl E.C.3.4.11.2
aminopeptidase,or membrane alanine
aminopeptidase
Aminopeptidase B
AminopeptidaseA,or
glutamyl-aminopeptidase
E.C.3.4.11.6
angiotensinase,or E.C.3.4.11.7
Dipeptidyl-peptidases and E.C.3.4.14.
tripeptidyl-peptidases （二肽基肽酶和三肽基
肽酶）
Dipeptidyl-peptidaseI,or cathepsin(组织蛋白
酶)C or J
Dipeptidyl-peptidase IV
Prolyl tripeptyl-peptidase
Peptidyl-Dipeptidases（肽基 - 二肽酶）
Peptidyl-depeptidaseA,
angiotensin-converting enzyme
Metallo-carboxypeptidases（金属羧肽酶）
Carboxypeptidase A
Carboxypeptidase B,or protaminase
Carboxypeptidase N,or
E.C.3.4.14.1
E.C.3.4.14.5
E.C.3.4.14.12
E.C.3.4.15.
or E.C.3.4.15.1
E.C.3.4.17.
E.C.3.4.17.1
E.C.3.4.17.2
Xaa-Yaa-|-Zaa,if Xaa≠ArgorLys,or
Yaa ≠Pro,orZaa≠Pro
Preferentially Xaa-Pro-|-Zaa,(but also
Xaa-Ala-|-Yaa)withYaa ≠Pro or Hyp
Xaa-Yaa-Pro-|-Zaa,if Zaa≠Pro
C-term
Xaa-|-Yaa-Zaa,if Yaa≠Pro or Zaa≠
Asp or Glu
C-term
Xaa-|-Yaaif Yaa ≠Asp,Glu,Arg,Lys or
Pro
Xaa-|-ArgandXaa-|-Lys
Xaa-|-Lys>> Xaa-|-Arg Lysine(arginine), E.C.3.4.17.3
Carboxypeptidase(羧基肽酶),or kininase I(激肽
酶)
Carboxypeptidase U or R
Glutamate
hydrolase
CarboxypeptidaseII,or
E.C.3.4.17.20 Xaa-|-Arg and Xaa-|-Lys
Xaa-|-Glu,preferentially with Xaa=
Asp or Glu
folate E.C.3.4.17.21
2024年5月30日发(作者：雪玉轩)
Enzymes
Serineproteases（丝氨酸蛋白酶）
α-chymotrypsin(胰凝乳蛋白酶，糜
蛋白酶)
Trypsin (胰朊酶，胰蛋白酶)
Pancreatic elastase (胰弹性蛋白酶)
Thrombin(凝血酶)
Plasmin(胞质素，胞浆素)
Prolyl oligopeptidase,or prolylendopeptidase
Plasma kallikrein (血浆激肽释放酶)
E.C. Number
E.C.3.4.21.
E.C.3.4.21.1
E.C.3.4.21.4
E.C.3.4.21.36
E.C.3.4.21.5
E.C.3.4.21.7
E.C.3.4.21.26
E.C.3.4.21.34
Cleavage sites
Tyr-|-Xaa,Trp-|-Xaa, Phe-|-Xaa, and
alsoLeu-|-Xaa,Met-|-Xaa,
Arg-|-Xaa, Lys-|-Xaa,
Ala-|-Xaa,andalsoGly-|-Xaa,
Val-|-Xaa, Ser-|-Xaa,
Arg-|-Gly,
Lys-|-Xaa,>Arg-|-Xaa
Arg-|-Xaa, Lys-|-Xaa,including
Arg-|-Ser,Lys-|-Arg,
Val-|-Xaa, Ala-|-Xaa,
Leukocyteelastase(白细胞弹性蛋白
酶),orneutrophilelastase(中性白细胞弹性蛋白
酶),orlysosomalelastase(溶酶体弹性蛋白酶)
Cysteine proteases（半胱氨酸蛋白酶）
Cathepsin B (组织蛋白酶)
E.C.3.4.21.37
E.C.3.4.22.
E.C.3.4.22.1
Arg-Arg-|-Xaa,and also Leu-|-Xaa,
Ala-|-Xaa, Phe-|-Xaa, Trp-|-Xaa,
Arg-|-Xaa including Arg-|-Pro,but not
Lys-|-Xaa
Met-|-Xaa,Tyr-|-Xaa and
Arg-|-Xaa(with LeuorVal as the P2
residue)
Preferentially Phe-|-Xaa, Tyr-|-Xaa,
And also Leu-|-Xaa,and Trp-|-Xaa,
ideally with Xaa= Phe,Trp,orTyr
Preferentially Phe-|-Xaa, Tyr-|-Xaa,
And also Leu-|-Xaa,ideally with Xaa
≠AlaorVal
Xaa-|-Tyr,Xaa-|-Phe, Xaa-|-Trp,and
Xaa-|-Leu,
Clostripain(梭菌蛋白酶),or endoproteinase
E.C.3.4.22.8
Arg-C
Calpain(钙激活中性蛋白酶)-1,or μ-calpain
E.C.3.4.22.52
Aspartic acid proteases (天冬氨酸蛋白酶)
Pepsin(胃朊酶，胃蛋白酶)
E.C.3.4.23.
E.C.3.4.23.1
Cathepsin(组织蛋白酶)D
E.C.3.4.23.5
Metalloproteases （金属蛋白酶）
E.C.3.4.24.
Neprilysin(脑啡肽酶),or enkephalinase(脑啡肽
E.C.3.4.24.11
酶),or neutralendopeptidase
Thimet oligopeptidase(甲拌磷寡肽 酶),or
E.C.3.4.24.15
endo-oligopeptidase A,or endopeptidase(肽链
内切 酶)24.15,or pz-peptidease(肠促胰 酶素)
Aminopeptidases （氨肽酶）
Leucyl-aminopeptidase
E.C.3.4.11.
E.C.3.4.11.1
Xaa-|-Arg, Xaa-|-Ser,Xaa-|-Ile,
Xaa-|-Ala, Xaa-|-Gly,
N-term
Preferentially Leu-|-Xaa,but not
Arg-|-Xaa,andLys-|-Xaa,
Preferentially Ala-|-Xaa,and
Tyr-|-Xaa,if Yaa-Pro-|-Xaa in term with
Yaa=Ala,Val,Leu,Ile,Phe,Tyr or Trp,then
the dipeptide Yaa-Pro could be
released
Arg-|-Xaa, Lys-|-Xaa,
Glu-|-Xaa>>Asp-|-Xaa,
N-term(di-and tripeptides)
Aminopeptidase M or N,or alanyl E.C.3.4.11.2
aminopeptidase,or membrane alanine
aminopeptidase
Aminopeptidase B
AminopeptidaseA,or
glutamyl-aminopeptidase
E.C.3.4.11.6
angiotensinase,or E.C.3.4.11.7
Dipeptidyl-peptidases and E.C.3.4.14.
tripeptidyl-peptidases （二肽基肽酶和三肽基
肽酶）
Dipeptidyl-peptidaseI,or cathepsin(组织蛋白
酶)C or J
Dipeptidyl-peptidase IV
Prolyl tripeptyl-peptidase
Peptidyl-Dipeptidases（肽基 - 二肽酶）
Peptidyl-depeptidaseA,
angiotensin-converting enzyme
Metallo-carboxypeptidases（金属羧肽酶）
Carboxypeptidase A
Carboxypeptidase B,or protaminase
Carboxypeptidase N,or
E.C.3.4.14.1
E.C.3.4.14.5
E.C.3.4.14.12
E.C.3.4.15.
or E.C.3.4.15.1
E.C.3.4.17.
E.C.3.4.17.1
E.C.3.4.17.2
Xaa-Yaa-|-Zaa,if Xaa≠ArgorLys,or
Yaa ≠Pro,orZaa≠Pro
Preferentially Xaa-Pro-|-Zaa,(but also
Xaa-Ala-|-Yaa)withYaa ≠Pro or Hyp
Xaa-Yaa-Pro-|-Zaa,if Zaa≠Pro
C-term
Xaa-|-Yaa-Zaa,if Yaa≠Pro or Zaa≠
Asp or Glu
C-term
Xaa-|-Yaaif Yaa ≠Asp,Glu,Arg,Lys or
Pro
Xaa-|-ArgandXaa-|-Lys
Xaa-|-Lys>> Xaa-|-Arg Lysine(arginine), E.C.3.4.17.3
Carboxypeptidase(羧基肽酶),or kininase I(激肽
酶)
Carboxypeptidase U or R
Glutamate
hydrolase
CarboxypeptidaseII,or
E.C.3.4.17.20 Xaa-|-Arg and Xaa-|-Lys
Xaa-|-Glu,preferentially with Xaa=
Asp or Glu
folate E.C.3.4.17.21