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细菌DNA的提取方法-USB迷|专注于互联网分享

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    细菌DNA的提取方法

    IT圈 admin 60浏览 0评论

    2024年6月10日发(作者：杜小溪)

    细菌DNA的提取方法

    -CAL-FENGHAI.-(YICAI)-Company One1

    细菌DNA的提取方法

    针对一些不易于提取的细菌的方法:

    试验试剂：

    抽提缓冲液：2%CTAB(W/V)2%PVPK25(w/v)（去色素）100mMTris-HCl

    25mMEDTA

    10MLiCl

    2MLiCl

    DEPC-water（抑制RNA酶活性）

    3MNaAc

    96%乙醇

    70%乙醇

    试验步骤：

    1、抽提缓冲液65℃预热。

    2、加菌体到已经预热的缓冲液（700ul）中混匀，65℃，10min。

    3、加酚/氯仿/异戊醇（25：24：1），振荡，以12,000rpm，离心10min。

    4、取上清，加氯仿/异戊醇（24：1）振荡，以12,000rpm，离心10min。

    5、重复再作一次步骤4。

    2

    2024年6月10日发(作者：杜小溪)

    细菌DNA的提取方法

    -CAL-FENGHAI.-(YICAI)-Company One1

    细菌DNA的提取方法

    针对一些不易于提取的细菌的方法:

    试验试剂：

    抽提缓冲液：2%CTAB(W/V)2%PVPK25(w/v)（去色素）100mMTris-HCl

    25mMEDTA

    10MLiCl

    2MLiCl

    DEPC-water（抑制RNA酶活性）

    3MNaAc

    96%乙醇

    70%乙醇

    试验步骤：

    1、抽提缓冲液65℃预热。

    2、加菌体到已经预热的缓冲液（700ul）中混匀，65℃，10min。

    3、加酚/氯仿/异戊醇（25：24：1），振荡，以12,000rpm，离心10min。

    4、取上清，加氯仿/异戊醇（24：1）振荡，以12,000rpm，离心10min。

    5、重复再作一次步骤4。

    2

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    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
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    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1726749913)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
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    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1726751127)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1726751127)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1727133654)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1727133654)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1727518388)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1727518388)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1728336202)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1728336202)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1729053684)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1729053684)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1729550013)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1729550013)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1729595873)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1729595873)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1729596632)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1729596632)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1729597854)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1729597854)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1729598778)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1729598778)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1095, humandate(1730459211)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(26))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)
    Error[2]: Trying to access array offset on value of type int, File: /www/wwwroot/www.usbmi.com/xiunophp/xiunophp.min.php, Line: 54
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 1096, humandate(1730459211)
    File: /www/wwwroot/www.usbmi.com/tmp/model_thread.func.php, Line: 662, well_thread_format(array(27))
    File: /www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm, Line: 249, well_thread_find_asc(array(20) , 20)
    File: /www/wwwroot/www.usbmi.com/tmp/route_read.php, Line: 204, include(/www/wwwroot/www.usbmi.com/tmp/view_template_d8_htm_read.htm)
    File: /www/wwwroot/www.usbmi.com/tmp/index.inc.php, Line: 129, include(/www/wwwroot/www.usbmi.com/tmp/route_read.php)
    File: /www/wwwroot/www.usbmi.com/index.php, Line: 29, include(/www/wwwroot/www.usbmi.com/tmp/index.inc.php)

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