2024年5月22日发(作者：元蕙兰)

常见载体引物表

序号

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

37

38

39

40

41

42

43

载体名称

pZeroBack/blunt

P3xFlag-CMV-14

P3xFlag-CMV-7.1

P3xFlag-MYC-CMV-24

pACT

pACT2

PACYCDUET-1(MCSI)

PACYCDUET-1(MCSII)

pAS2-1

PB42AD

pBabe-puro

PBACPAK8

pBAD

P

BIND

pBK-CMV

pBlueScript II SK(+)

pBluescript SK (+)

pBluescript SK (-)

pBluescript KS (+)

pBluescript KS (-)

pBV220

PCANTB5E

pCAT3-enhancer

pcDNA3.0

pcDNA3.1

pcDNA4.0

pcDNA6/V5-His

pcDNA6/myc-His

pcDNAII

pCEP4

pCI-neo

pCMS-EGFP

pCMV-3Tag-4A

pCMV5-Flag

pCMV-MYC/NUC

pCMV-Sport

pCMV-Tag

pCMV-TNT

PCOLDI

pCR2.1-TOPO

pCR3.1

pCS2

PDNR-LIB

正向引物(多个引物可根

据酶切位点选用)

PJET1.2-F

CMV-30

CMV-F

CMV-F

T7

5’AD

DuetUP1

DuetUP2

5’BD

PB42ADF

pBABE5’

BAC1

PBAD-F

5’BD

T7/M13-47

M

13-47

M

13-47

M

13-47

M

13-47

M

13-47

PBV220+

S1

pGL3+

pEGFP-N5/T7

pEGFP-N5/T7

pEGFP-N5/T7

T7

T7

T7

pCMV5R/PCEP-F

T7

T7

T3

pCMV5F

pCMV5F

M13-20/T7

T3

T7/SP6

pCold-F

M13-47

T7

SP6

M13-20

反向引物(多个引物可根

据酶切位点选用)

PJET1.2-R

CMV-24

CMV-24

CMV-24

T3

3'AD

DuetDOWN1

T7 Ter

3'BD

PB42ADR

pBABE3’

BAC2

PBAD-R

T

3

T3/M13-48

M

13-48

M

13-48

M

13-48

M

13-48

M

13-48

PBV220-

S6

SP6/BGH

BGH

BGH

BGH

BGH

SP6

pEGFP-N5/EBV-R

T3

T3

T7

pCMV5R

pCMV5R

SP6/M13-26

T7

pCMV5R

pCold-R

M13-48

BGH

T7

M13-26

44

45

46

47

48

49

50

51

52

53

54

55

56

57

58

59

60

61

62

63

64

65

66

67

68

69

70

71

72

73

74

75

76

77

78

79

80

81

82

83

84

85

86

87

88

89

pDONR/Zeo

pDONR221

pDrive

pDsRED1-C1

pDSRED2-N1

pDsRed-Monomer-N1

pEASY-BLUNT

pEASY-E1

pEASY-T1

pEASY-T3

pECFP-C1

pECFP-N1

pENTR/D-TOPO

pET-*(His)

M13-20

M13-20

T7

pDsRED-C1-F

pEGFP-N-5

pEGFP-N-5

M13-20

T7

M13-47/T7

M13-47/T7

pEGFP-C-5’

pEGFP-N-5’

M13-20

T7

M13-26

M13-26

SP6

pECFP-C-3

pDsRed1-N

pDsRed1-N

M13-26

T7 Ter

M13-48

M13-48/SP6

pECFP-C-3'

pEGFP-N-3’

M13-26

T7 Ter

PET-15B

pET22(a,b,c)

pET28,30,24,49

pET32(a,b,c)

pET-42a (-b, -c)(+)

pET44

pET50

T7

T7

T7

T7/

Stag

T7/

T7/

T7 Ter

T7 Ter

T7 Ter

T7 Ter

T7 Ter

T7 Ter

T7 Ter

DuetDOMN1

T7 Ter

pEGFP-C3'

pEGFP-N-3’

pECFP-C3

pECFP-C3

pECFP-C3

CMV-24/pFLAG-CMV-R

3'AD

3'AD

3'AD

3'AOX

3'BD

M13-48

pGEX-3’

pGEX-3’

G

LP2

G

LP2

PGL3-

PGL3-

GLP2

pIRES2-EGFP.P3’

pJET1.2R

PLEXA-R

PETA-IRES

PETDUET-1(MCSI)

PETDUET-1(MCSII)

pEYFP-C1

pEYFP-N1

pFastBac Dual (MCSI)

pFastBac HT_A,B,C

pFastBac1

pFLAG-CMV

pGAD424

pGADGH

pGADT7-Rec

pGAPZa-A/B/C

pGBKT7

pGEM-T(-Easy)

pGEX-(*)T

PGEX-6P-

1

pGL2-Basic

pGL2-Control

Pgl3-BASIC

pGL3-Enhancer

PGL4.10

pIRES2-EGFP

pJET1.2/blunt

PLEXA

EF-1aF

DuetUP1

DuetUP2

pEGFP-C5’

pEGFP-N5’

pFastBac-PH

pFastBac-PH

pFastBac-PH

CMV30/pFLAG-CMV-F

5’AD

5’AD

5’AD/T7

a-FACTOR

T7

M13-47

pGEX-5’

pGEX-5’

G

LP1

G

LP1

pGL3+

pGL3+

R

VP3

pIRES2-EGFP.P5’

T7

/pJET1.2F

PLEXA-F

90

91

92

93

94

95

96

97

98

99

100

101

102

103

104

105

106

107

108

109

110

111

112

113

114

115

116

117

118

119

120

121

122

123

124

125

126

127

128

129

130

131

132

133

134

135

PLK0.1

PLL3.7

PLNCX

PLVX-SHRNA1

PLVX-IRES-NEO

pLXSN

pMAL-c2E

pMAL-C2x

pMAL-P2x

PMD18-T

PMD19-T

pmirGLO

pMIR-report

pmscv

pPIC9K

pPICZa

pPROEXHTa/b/c

pQE2

pQE30

pQE40

pRSET

PRSFDUET-1(MCSI)

PRSFDUET-1(MCSII)

pSectag(v5/His/a,b,c)

psi-check2

pSilencer3.1-H1 hygro

pSILENCEV2.0-U6

PsiREN-retroq

pSK01-T

pSK-CMV

PSP64/65

pSP72

pSport1

pSTBlue-1

pSUPER

pT7Blue(R)

pTA2

pT-Adv

PTHIOHISA,B,C

pTO-T7

pTRC99a-c

pUC18(19)/118(119)

pUC57

pUCm-T

pVAX1

pXT7

人源U6

鼠源U6

PLNCX-F

人源U6

PEGFP-N5

pLXSN-F

MalE primer

PIRES2-EGFP.P5/PMAL-

C2X-F

MalE primer

M13-48

M13-47

M13-47

MSCV-F

5’AOX/a-factor

5'AOX

M13-48

pQE30+

pQE30+

pQE30+

T7

DuetUP1

DuetUP2

T

7

psi-check2-F

M13-47

T7

人源U

6

M13-20

T7

SP6

T7

SP6

T7

T7

T7

M13-47

M13-47 /T7

TRX-FORWARD

T7

PQE30+/PTRC99C-F

M13-47

M13-26/M13-48

M13-47/T7

T7

T7

PLNCX-R

pLXSN-R

M13-47

PIRES2-EGFP.P5/PMAL-

C2X-R

M13-47

M13-47

M13-48

T7 Ter

MSCV-R

3’AOX

3'AOX

PBV220-

pQE30-

pQE30-

pQE30-

T7 Ter

DuetDOWN1

T7 Ter

BGH

psi-check2-R

3.0rev

2.0rev

M13-26

T3

SP6

T7

SP6

T3

M13-47

T3/M13-48

M13-48

TRX-REVERSE

T7 Ter

PBV220-/PTRC99C-R

M13-48

M13-20/M13-47

M13-48

BGH

SP6

136

pYES2T7pYes2.R

2024年5月22日发(作者：元蕙兰)

常见载体引物表

序号

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

37

38

39

40

41

42

43

载体名称

pZeroBack/blunt

P3xFlag-CMV-14

P3xFlag-CMV-7.1

P3xFlag-MYC-CMV-24

pACT

pACT2

PACYCDUET-1(MCSI)

PACYCDUET-1(MCSII)

pAS2-1

PB42AD

pBabe-puro

PBACPAK8

pBAD

P

BIND

pBK-CMV

pBlueScript II SK(+)

pBluescript SK (+)

pBluescript SK (-)

pBluescript KS (+)

pBluescript KS (-)

pBV220

PCANTB5E

pCAT3-enhancer

pcDNA3.0

pcDNA3.1

pcDNA4.0

pcDNA6/V5-His

pcDNA6/myc-His

pcDNAII

pCEP4

pCI-neo

pCMS-EGFP

pCMV-3Tag-4A

pCMV5-Flag

pCMV-MYC/NUC

pCMV-Sport

pCMV-Tag

pCMV-TNT

PCOLDI

pCR2.1-TOPO

pCR3.1

pCS2

PDNR-LIB

正向引物(多个引物可根

据酶切位点选用)

PJET1.2-F

CMV-30

CMV-F

CMV-F

T7

5’AD

DuetUP1

DuetUP2

5’BD

PB42ADF

pBABE5’

BAC1

PBAD-F

5’BD

T7/M13-47

M

13-47

M

13-47

M

13-47

M

13-47

M

13-47

PBV220+

S1

pGL3+

pEGFP-N5/T7

pEGFP-N5/T7

pEGFP-N5/T7

T7

T7

T7

pCMV5R/PCEP-F

T7

T7

T3

pCMV5F

pCMV5F

M13-20/T7

T3

T7/SP6

pCold-F

M13-47

T7

SP6

M13-20

反向引物(多个引物可根

据酶切位点选用)

PJET1.2-R

CMV-24

CMV-24

CMV-24

T3

3'AD

DuetDOWN1

T7 Ter

3'BD

PB42ADR

pBABE3’

BAC2

PBAD-R

T

3

T3/M13-48

M

13-48

M

13-48

M

13-48

M

13-48

M

13-48

PBV220-

S6

SP6/BGH

BGH

BGH

BGH

BGH

SP6

pEGFP-N5/EBV-R

T3

T3

T7

pCMV5R

pCMV5R

SP6/M13-26

T7

pCMV5R

pCold-R

M13-48

BGH

T7

M13-26

44

45

46

47

48

49

50

51

52

53

54

55

56

57

58

59

60

61

62

63

64

65

66

67

68

69

70

71

72

73

74

75

76

77

78

79

80

81

82

83

84

85

86

87

88

89

pDONR/Zeo

pDONR221

pDrive

pDsRED1-C1

pDSRED2-N1

pDsRed-Monomer-N1

pEASY-BLUNT

pEASY-E1

pEASY-T1

pEASY-T3

pECFP-C1

pECFP-N1

pENTR/D-TOPO

pET-*(His)

M13-20

M13-20

T7

pDsRED-C1-F

pEGFP-N-5

pEGFP-N-5

M13-20

T7

M13-47/T7

M13-47/T7

pEGFP-C-5’

pEGFP-N-5’

M13-20

T7

M13-26

M13-26

SP6

pECFP-C-3

pDsRed1-N

pDsRed1-N

M13-26

T7 Ter

M13-48

M13-48/SP6

pECFP-C-3'

pEGFP-N-3’

M13-26

T7 Ter

PET-15B

pET22(a,b,c)

pET28,30,24,49

pET32(a,b,c)

pET-42a (-b, -c)(+)

pET44

pET50

T7

T7

T7

T7/

Stag

T7/

T7/

T7 Ter

T7 Ter

T7 Ter

T7 Ter

T7 Ter

T7 Ter

T7 Ter

DuetDOMN1

T7 Ter

pEGFP-C3'

pEGFP-N-3’

pECFP-C3

pECFP-C3

pECFP-C3

CMV-24/pFLAG-CMV-R

3'AD

3'AD

3'AD

3'AOX

3'BD

M13-48

pGEX-3’

pGEX-3’

G

LP2

G

LP2

PGL3-

PGL3-

GLP2

pIRES2-EGFP.P3’

pJET1.2R

PLEXA-R

PETA-IRES

PETDUET-1(MCSI)

PETDUET-1(MCSII)

pEYFP-C1

pEYFP-N1

pFastBac Dual (MCSI)

pFastBac HT_A,B,C

pFastBac1

pFLAG-CMV

pGAD424

pGADGH

pGADT7-Rec

pGAPZa-A/B/C

pGBKT7

pGEM-T(-Easy)

pGEX-(*)T

PGEX-6P-

1

pGL2-Basic

pGL2-Control

Pgl3-BASIC

pGL3-Enhancer

PGL4.10

pIRES2-EGFP

pJET1.2/blunt

PLEXA

EF-1aF

DuetUP1

DuetUP2

pEGFP-C5’

pEGFP-N5’

pFastBac-PH

pFastBac-PH

pFastBac-PH

CMV30/pFLAG-CMV-F

5’AD

5’AD

5’AD/T7

a-FACTOR

T7

M13-47

pGEX-5’

pGEX-5’

G

LP1

G

LP1

pGL3+

pGL3+

R

VP3

pIRES2-EGFP.P5’

T7

/pJET1.2F

PLEXA-F

90

91

92

93

94

95

96

97

98

99

100

101

102

103

104

105

106

107

108

109

110

111

112

113

114

115

116

117

118

119

120

121

122

123

124

125

126

127

128

129

130

131

132

133

134

135

PLK0.1

PLL3.7

PLNCX

PLVX-SHRNA1

PLVX-IRES-NEO

pLXSN

pMAL-c2E

pMAL-C2x

pMAL-P2x

PMD18-T

PMD19-T

pmirGLO

pMIR-report

pmscv

pPIC9K

pPICZa

pPROEXHTa/b/c

pQE2

pQE30

pQE40

pRSET

PRSFDUET-1(MCSI)

PRSFDUET-1(MCSII)

pSectag(v5/His/a,b,c)

psi-check2

pSilencer3.1-H1 hygro

pSILENCEV2.0-U6

PsiREN-retroq

pSK01-T

pSK-CMV

PSP64/65

pSP72

pSport1

pSTBlue-1

pSUPER

pT7Blue(R)

pTA2

pT-Adv

PTHIOHISA,B,C

pTO-T7

pTRC99a-c

pUC18(19)/118(119)

pUC57

pUCm-T

pVAX1

pXT7

人源U6

鼠源U6

PLNCX-F

人源U6

PEGFP-N5

pLXSN-F

MalE primer

PIRES2-EGFP.P5/PMAL-

C2X-F

MalE primer

M13-48

M13-47

M13-47

MSCV-F

5’AOX/a-factor

5'AOX

M13-48

pQE30+

pQE30+

pQE30+

T7

DuetUP1

DuetUP2

T

7

psi-check2-F

M13-47

T7

人源U

6

M13-20

T7

SP6

T7

SP6

T7

T7

T7

M13-47

M13-47 /T7

TRX-FORWARD

T7

PQE30+/PTRC99C-F

M13-47

M13-26/M13-48

M13-47/T7

T7

T7

PLNCX-R

pLXSN-R

M13-47

PIRES2-EGFP.P5/PMAL-

C2X-R

M13-47

M13-47

M13-48

T7 Ter

MSCV-R

3’AOX

3'AOX

PBV220-

pQE30-

pQE30-

pQE30-

T7 Ter

DuetDOWN1

T7 Ter

BGH

psi-check2-R

3.0rev

2.0rev

M13-26

T3

SP6

T7

SP6

T3

M13-47

T3/M13-48

M13-48

TRX-REVERSE

T7 Ter

PBV220-/PTRC99C-R

M13-48

M13-20/M13-47

M13-48

BGH

SP6

136

pYES2T7pYes2.R