2024年5月22日发(作者：吕念云)
pCDH-CMV-MCS-EF1-Neo
编号
北京华越洋VECT231266
载体名称
pCDH-CMV-MCS-EF1-Neo
pCDH-CMV-MCS-EF1-Neo载体基本信息：
pCDH-CMV-MCS-EF1-Neo
载体名称:
质粒类型: 慢病毒表达载体；cDNA表达载体；双启动子载体
克隆方法: 多克隆位点，限制性内切酶
CMV
启动子:
--
载体大小:
CMV-F :CGCAAATGGGCGGTAGGCGTG
5' 测序引物及序列:
--
3' 测序引物及序列:
载体标签: 无
载体抗性: 氨苄青霉素（Ampicillin）
Neomycin
筛选标记:
克隆菌株: cells(RecA-)推荐: Stbl2 ,OmniMAX 2 T1R
宿主细胞（系）: 常用细胞系，如HeLa, HEK293, HT1080, H1299
pCDH-CMV-MCS-EF1-Neo慢病毒表达载体是基于HIV-1的
慢病毒载体；
备注: 用于cDNA表达和克隆；高效转染细胞，建立稳定细胞系；
CMV启动子驱动目的基因的高水平表达，EF1a启动子驱
动报告基因的中等水平的表达。
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒（HIV)
pCDH-CMV-MCS-EF1-Neo载体质粒图谱和多克隆位点信息：
pCDH-CMV-MCS-EF1-Neo载体简介：
背景简介：
This manual provides details and information necessary to generate expression constructs of your
gene of interest in the pCDH cDNA Cloning and Expression Lentivectors. Specifically, it provides
critical instructions on amplification and cloning cDNA into the pCDH vectors, and verification of the
final expression constructs. This manual does not include information on packaging the pCDH
expression constructs into pseudotyped viral particles or transducing your target cells of choice with
these particles. This information is available in the user manual Lentivector Expression Systems: Guide
to Packaging and Transduction of Target Cells which is available on the SBI website. Before using the
reagents and material supplied with this system, please read the entire manual.
基于HIV-1的pCDH 慢病毒载体特征：
Multiple Cloning Site (MCS)—for cloning the gene of interest in the MCS located downstream of the
CMV promoter.
WPRE element—enhances stability and translation of the CMV-driven transcripts.
SV40 polyadenylation signal—enables efficient termination of transcription and processing of
recombinant transcripts.
Hybrid RSV/5LTR promoter—provides a high level of expression of the full-length viral transcript in
producer 293 cells.
Genetic elements (cPPT, gag, env, LTRs)—necessary for packaging, transducing, and stably
integrating the vira expression construct into genomic DNA.
SV40 origin—for stable propagation of the pCDH plasmid in mammalian cells.
2024年5月22日发(作者：吕念云)
pCDH-CMV-MCS-EF1-Neo
编号
北京华越洋VECT231266
载体名称
pCDH-CMV-MCS-EF1-Neo
pCDH-CMV-MCS-EF1-Neo载体基本信息：
pCDH-CMV-MCS-EF1-Neo
载体名称:
质粒类型: 慢病毒表达载体；cDNA表达载体；双启动子载体
克隆方法: 多克隆位点，限制性内切酶
CMV
启动子:
--
载体大小:
CMV-F :CGCAAATGGGCGGTAGGCGTG
5' 测序引物及序列:
--
3' 测序引物及序列:
载体标签: 无
载体抗性: 氨苄青霉素（Ampicillin）
Neomycin
筛选标记:
克隆菌株: cells(RecA-)推荐: Stbl2 ,OmniMAX 2 T1R
宿主细胞（系）: 常用细胞系，如HeLa, HEK293, HT1080, H1299
pCDH-CMV-MCS-EF1-Neo慢病毒表达载体是基于HIV-1的
慢病毒载体；
备注: 用于cDNA表达和克隆；高效转染细胞，建立稳定细胞系；
CMV启动子驱动目的基因的高水平表达，EF1a启动子驱
动报告基因的中等水平的表达。
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒（HIV)
pCDH-CMV-MCS-EF1-Neo载体质粒图谱和多克隆位点信息：
pCDH-CMV-MCS-EF1-Neo载体简介：
背景简介：
This manual provides details and information necessary to generate expression constructs of your
gene of interest in the pCDH cDNA Cloning and Expression Lentivectors. Specifically, it provides
critical instructions on amplification and cloning cDNA into the pCDH vectors, and verification of the
final expression constructs. This manual does not include information on packaging the pCDH
expression constructs into pseudotyped viral particles or transducing your target cells of choice with
these particles. This information is available in the user manual Lentivector Expression Systems: Guide
to Packaging and Transduction of Target Cells which is available on the SBI website. Before using the
reagents and material supplied with this system, please read the entire manual.
基于HIV-1的pCDH 慢病毒载体特征：
Multiple Cloning Site (MCS)—for cloning the gene of interest in the MCS located downstream of the
CMV promoter.
WPRE element—enhances stability and translation of the CMV-driven transcripts.
SV40 polyadenylation signal—enables efficient termination of transcription and processing of
recombinant transcripts.
Hybrid RSV/5LTR promoter—provides a high level of expression of the full-length viral transcript in
producer 293 cells.
Genetic elements (cPPT, gag, env, LTRs)—necessary for packaging, transducing, and stably
integrating the vira expression construct into genomic DNA.
SV40 origin—for stable propagation of the pCDH plasmid in mammalian cells.